##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511795_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2167955 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.237777998159554 31.0 30.0 34.0 23.0 34.0 2 26.52148130380935 31.0 26.0 34.0 7.0 34.0 3 28.246912874114084 31.0 28.0 34.0 16.0 34.0 4 32.579980673030576 35.0 32.0 37.0 25.0 37.0 5 32.93763754321469 35.0 35.0 37.0 26.0 37.0 6 33.1333879162621 37.0 35.0 37.0 27.0 37.0 7 33.10320555546586 37.0 35.0 37.0 26.0 37.0 8 33.11840005904182 37.0 35.0 37.0 26.0 37.0 9 34.64192614699106 39.0 35.0 39.0 27.0 39.0 10 34.50977534127784 39.0 35.0 39.0 27.0 39.0 11 34.51481557504653 39.0 35.0 39.0 27.0 39.0 12 34.38760675383022 39.0 35.0 39.0 26.0 39.0 13 34.37663051124216 39.0 35.0 39.0 26.0 39.0 14 35.45595549723126 40.0 36.0 41.0 26.0 41.0 15 35.39337947512748 40.0 36.0 41.0 25.0 41.0 16 35.34252325348081 40.0 36.0 41.0 25.0 41.0 17 35.29715745944911 40.0 36.0 41.0 25.0 41.0 18 35.2588033423203 40.0 36.0 41.0 25.0 41.0 19 35.273119137620476 40.0 36.0 41.0 25.0 41.0 20 35.20535158709475 40.0 36.0 41.0 25.0 41.0 21 35.09838073207239 40.0 36.0 41.0 24.0 41.0 22 34.99079547315327 40.0 35.0 41.0 23.0 41.0 23 34.870141216030774 40.0 35.0 41.0 22.0 41.0 24 34.75386573983316 40.0 35.0 41.0 19.0 41.0 25 34.64810893215034 39.0 35.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0824251159894125 1101 2 3.6512883277805415 1101 3 2.2954640100207833 1101 4 1.3724524872153694 1101 5 0.6985659223905714 1101 6 0.8535349971650277 1101 7 0.6253617124394566 1101 8 0.5685059612165233 1101 9 0.784313001400136 1101 10 0.6743117290094105 1101 11 0.8081130302271831 1101 12 0.6248592593867031 1101 13 0.5511836837206587 1101 14 0.596344740187206 1101 15 0.7648652793423878 1101 16 0.5978522290908757 1101 17 0.7463853798270321 1101 18 0.8760459494313935 1101 19 0.9064859581136062 1101 20 0.9449274243389283 1101 21 0.8967161615640293 1101 22 0.8170350589927438 1101 23 0.7490453487217508 1101 24 0.8174585831335577 1101 25 0.8778888225534729 1102 1 1.7861343483294085 1102 2 4.432413710104068 1102 3 2.8471573396813916 1102 4 2.0128459746162193 1102 5 1.584067345571512 1102 6 1.515361694027213 1102 7 1.4233219755341224 1102 8 1.3324220427488171 1102 9 1.568045578635946 1102 10 1.5642294940572725 1102 11 1.5096241256504044 1102 12 1.5893292186403443 1102 13 1.5394460834286008 1102 14 1.7785198730898202 1102 15 1.6046437502462112 1102 16 1.8565223067734138 1102 17 1.6664216892222257 1102 18 1.6980702586924323 1102 19 1.698772190947878 1102 20 1.7412151680486971 1102 21 1.747318724884174 1102 22 1.9740976597108997 1102 23 1.9930388213877137 1102 24 1.5076026496208783 1102 25 1.5505005753834737 1103 1 1.312195608298481 1103 2 3.725955597549735 1103 3 2.2772935263064795 1103 4 1.5111331031071913 1103 5 1.0247886512385023 1103 6 0.8198440813325405 1103 7 1.0672597444042609 1103 8 0.9281000570558291 1103 9 0.7009878863487771 1103 10 0.9698993655545536 1103 11 1.0336214021313452 1103 12 0.936437560698117 1103 13 1.1935319489836616 1103 14 1.3495544154649792 1103 15 1.1782950907149115 1103 16 1.2495996378527963 1103 17 1.2296421224453127 1103 18 1.2747332648299547 1103 19 1.209278870079551 1103 20 1.2966551092240977 1103 21 1.259640727652517 1103 22 1.34759329428978 1103 23 1.256707909553107 1103 24 1.350147603159904 1103 25 1.2367537409941392 1104 1 1.2208587024673463 1104 2 3.7951978330411684 1104 3 2.2011998854922865 1104 4 1.4982116379093995 1104 5 1.1974910432239483 1104 6 1.08927918672088 1104 7 0.874887741159263 1104 8 0.9610047451796362 1104 9 1.152525810357119 1104 10 1.1564106389610558 1104 11 1.0861302699037765 1104 12 1.2094305099308542 1104 13 1.246789701990572 1104 14 1.4026710815608183 1104 15 1.380528097793281 1104 16 1.2896683205968955 1104 17 1.2093273963967377 1104 18 1.1401573102313165 1104 19 1.1645170028045086 1104 20 1.222335789671959 1104 21 1.1989235154071949 1104 22 1.286882216257979 1104 23 1.2665730028621596 1104 24 1.2359806881427886 1104 25 1.2559926793458018 1105 1 1.2668877023025757 1105 2 3.8289307379678448 1105 3 2.26944836135322 1105 4 1.332686015895888 1105 5 1.0610023300916325 1105 6 0.8785195887640498 1105 7 0.6176460209417627 1105 8 0.6632785958963936 1105 9 0.8252437210508035 1105 10 0.8963760368849307 1105 11 0.9726272216256362 1105 12 0.6065634807662477 1105 13 0.8354987150075459 1105 14 0.8509656935232499 1105 15 0.8456912218664385 1105 16 0.943828808321399 1105 17 0.9302245787991055 1105 18 0.9555263990433076 1105 19 1.0124202817631485 1105 20 0.9899251783686154 1105 21 1.1719833275673537 1105 22 1.1168995172960408 1105 23 1.093348418910935 1105 24 1.1263332997756805 1105 25 1.1470950026280562 1106 1 1.0478882158163714 1106 2 3.6979896977356113 1106 3 2.18964622136307 1106 4 1.064696527208909 1106 5 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0.8429873097907858 1107 18 0.5667585897613279 1107 19 0.6408551809026619 1107 20 0.6131019310840884 1107 21 0.6112131202038356 1107 22 0.7656969499381603 1107 23 0.737092247377575 1107 24 0.8766859588249076 1107 25 0.8317472808055157 1108 1 1.042363527080493 1108 2 3.5677833243528347 1108 3 1.9827013950449377 1108 4 1.2613125237596847 1108 5 0.9224805081700111 1108 6 0.6497078258545841 1108 7 0.5760896090962149 1108 8 0.6237512320420109 1108 9 0.5345992131331201 1108 10 0.5501943117899444 1108 11 0.5746001317241749 1108 12 0.5495507352191069 1108 13 0.6319213839376943 1108 14 0.7072535866007783 1108 15 0.6870745044511182 1108 16 0.5811178421209036 1108 17 0.5567403019000778 1108 18 0.5824916743884501 1108 19 0.6169604899673686 1108 20 0.6566861566948958 1108 21 0.686103130321257 1108 22 0.6530444608437804 1108 23 0.6934139879695209 1108 24 0.8008830738767969 1108 25 0.832527893145695 1109 1 0.7552514071944572 1109 2 2.9317104148810778 1109 3 1.6334635427498725 1109 4 1.0464283791800497 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-0.3545615489185536 2314 7 -0.4368547865267658 2314 8 -0.3809817408069236 2314 9 -0.49481781616280074 2314 10 -0.5733925625443135 2314 11 -0.5900504030157947 2314 12 -0.6921423573418437 2314 13 -0.7326005630639685 2314 14 -1.4459592893318671 2314 15 -1.155647737937116 2314 16 -1.1101506473194505 2314 17 -1.1501876726001328 2314 18 -0.8197906890555586 2314 19 -0.808059369292458 2314 20 -0.7513788648733524 2314 21 -0.7244726598671107 2314 22 -0.7270627197236266 2314 23 -0.704391406616871 2314 24 -0.7970681706282008 2314 25 -0.7506485099706666 2315 1 -4.845451614088635 2315 2 -13.697128144799764 2315 3 -7.490003092944512 2315 4 -3.84818004654117 2315 5 -2.5050615879227003 2315 6 -1.8049692448358527 2315 7 -1.6366013577680292 2315 8 -1.5781999894954701 2315 9 -1.6177928289166132 2315 10 -1.6371986921003199 2315 11 -1.7422282371741957 2315 12 -1.6778864925765617 2315 13 -1.714846347488752 2315 14 -1.7630559876567702 2315 15 -1.9725467379700632 2315 16 -1.975823463463449 2315 17 -1.8644039977070221 2315 18 -1.916831577379888 2315 19 -2.045104450184006 2315 20 -1.966186722585121 2315 21 -2.105762362853831 2315 22 -1.9784887890839116 2315 23 -1.9297870588451218 2315 24 -2.072096888009405 2315 25 -2.092784151450495 2316 1 -1.4484705448728477 2316 2 -1.4187810038609925 2316 3 -1.0334555476699308 2316 4 -1.2423453477974995 2316 5 -1.3904573730971315 2316 6 -1.4657039568830363 2316 7 -1.3945260439700142 2316 8 -1.561413117446829 2316 9 -1.571434494834719 2316 10 -1.760442799042366 2316 11 -1.8294158462601544 2316 12 -1.8356911629052988 2316 13 -1.8084541804148344 2316 14 -2.0480513314274873 2316 15 -2.287864424705326 2316 16 -2.1467812708627036 2316 17 -1.9606878832877186 2316 18 -2.0817035964550783 2316 19 -1.9653245948852884 2316 20 -2.245121719577618 2316 21 -2.1979495910272604 2316 22 -2.0865779783314906 2316 23 -1.8697759953259663 2316 24 -1.8768078663633432 2316 25 -1.9775944968073986 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 95078.0 3 2.0 4 893.0 5 4211.0 6 5068.0 7 4457.0 8 3987.0 9 3986.0 10 3851.0 11 3694.0 12 3495.0 13 3553.0 14 3609.0 15 3672.0 16 3787.0 17 4226.0 18 4805.0 19 5838.0 20 7258.0 21 9407.0 22 11729.0 23 13731.0 24 16044.0 25 18289.0 26 20292.0 27 22723.0 28 26077.0 29 31195.0 30 38627.0 31 48729.0 32 61665.0 33 80814.0 34 108910.0 35 155094.0 36 239359.0 37 322558.0 38 777242.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.819498098438395 18.534517552255465 12.416032620603287 25.229951728702854 2 16.36645341375457 20.825879241268584 39.89239755808336 22.915269786893486 3 19.020228740910213 24.440129061719453 29.46301929698725 27.07662290038308 4 12.293705358275425 16.386779245879183 38.35674633467946 32.962769061165936 5 13.095328701288459 37.88880785962348 35.76273524048856 13.253128198599502 6 31.962103994911285 37.08620146471316 17.920480014834407 13.031214525541149 7 28.171110562719242 31.445117633899226 22.17536803116301 18.20840377221852 8 24.95923577749538 37.41433747471696 20.345302370206024 17.281124377581637 9 26.53740506606456 15.132417416413164 20.088147586089196 38.24202993143308 10 14.929092162890836 28.928137346024247 34.67041520695771 21.472355284127207 11 34.608651932350995 22.22919756175751 24.64087123579595 18.52127927009555 12 23.511558127359656 24.334084425184102 31.862284964401937 20.29207248305431 13 29.16317835303625 21.866156692511325 25.741932597247263 23.22873235720516 14 20.88558111215408 22.109960769480917 27.585489551213012 29.418968567151992 15 22.909931248572963 31.108856041753636 23.293241787767734 22.687970921905666 16 22.118817041866645 28.074614094849753 27.801222811359093 22.00534605192451 17 21.689879157283727 28.646661211416173 27.802542687950137 21.860916943349963 18 21.536747764598434 27.368464751344007 29.901450906499445 21.193336577558114 19 23.437565586243576 26.80171609361299 27.638452416041538 22.122265904101898 20 23.53268676007015 27.08817855828246 28.340564461595374 21.038570220052012 21 22.95425422682752 26.669351245284687 27.327488147723784 23.04890638016401 22 23.19621155299204 27.880014059987435 27.48252419212733 21.4412501948932 23 22.29917664152771 27.613598099587165 28.000691897875875 22.08653336100925 24 23.134014759885474 26.627436744171675 28.600726139499784 21.63782235644307 25 22.666177722118054 27.271620228466258 28.202598325134286 21.859603724281403 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 225.0 1 225.0 2 364.0 3 503.0 4 503.0 5 503.0 6 1413.0 7 2323.0 8 2323.0 9 2323.0 10 3424.5 11 4526.0 12 4526.0 13 4526.0 14 7055.0 15 9584.0 16 9584.0 17 9584.0 18 17438.5 19 25293.0 20 25293.0 21 25293.0 22 39929.5 23 54566.0 24 54566.0 25 54566.0 26 77698.0 27 100830.0 28 100830.0 29 100830.0 30 130847.0 31 160864.0 32 160864.0 33 160864.0 34 183359.5 35 205855.0 36 205855.0 37 205855.0 38 222927.0 39 239999.0 40 239999.0 41 239999.0 42 254079.0 43 268159.0 44 268159.0 45 268159.0 46 274208.0 47 280257.0 48 280257.0 49 280257.0 50 272516.0 51 264775.0 52 264775.0 53 264775.0 54 236467.0 55 208159.0 56 208159.0 57 208159.0 58 179934.5 59 151710.0 60 151710.0 61 151710.0 62 124165.5 63 96621.0 64 96621.0 65 96621.0 66 74835.0 67 53049.0 68 53049.0 69 53049.0 70 38964.5 71 24880.0 72 24880.0 73 24880.0 74 17506.5 75 10133.0 76 10133.0 77 10133.0 78 7016.5 79 3900.0 80 3900.0 81 3900.0 82 2568.5 83 1237.0 84 1237.0 85 1237.0 86 808.5 87 380.0 88 380.0 89 380.0 90 235.0 91 90.0 92 90.0 93 90.0 94 57.5 95 25.0 96 25.0 97 25.0 98 18.5 99 12.0 100 12.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.4772815856417685 3 0.0 4 0.0 5 6.457698614593015E-4 6 0.0010147812108646165 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 9.225283735132879E-5 14 0.0 15 0.0 16 0.0 17 5.996434427836371E-4 18 0.0 19 3.6901134940531515E-4 20 5.996434427836371E-4 21 7.841491174862947E-4 22 0.004658768286242104 23 2.3063209337832196E-4 24 0.0023985737711345483 25 9.225283735132878E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2167955.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.82233392907557 #Duplication Level Percentage of deduplicated Percentage of total 1 85.86210561344863 64.24403138063009 2 9.738658619950876 14.573383345664691 3 2.2500949908126837 5.050720763241806 4 0.8523924391698268 2.5511196688873605 5 0.39381442339774403 1.4733057146776176 6 0.23389243588803266 1.0500226764899567 7 0.15192370460255009 0.7957100310259967 8 0.10145276876711638 0.6072746354177977 9 0.07663923903772193 0.5160894061815607 >10 0.30834532430465383 3.8335861558323923 >50 0.016341630431961997 0.8383622141146568 >100 0.011473428406447864 1.6912429555911614 >500 0.001744145432413677 0.8932329562884314 >1k 0.0010589454411078724 1.6324280873463368 >5k 6.22909083004631E-5 0.24949000860999118 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5353 0.24691471917083152 No Hit GTCCTACAGTGGACATTTCTAAATT 3976 0.18339864065444164 No Hit GGTATCAACGCAGAGTACTTTTTTT 3565 0.16444068257874356 No Hit CTGTAGGACGTGGAATATGGCAAGA 3359 0.1549386403315567 No Hit TATCAACGCAGAGTACTTTTTTTTT 3140 0.1448369546415862 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3115 0.1436837941746946 No Hit CTTTAGGACGTGAAATATGGCGAGG 2761 0.12735504196350939 No Hit GTCCTACAGTGTGCATTTCTCATTT 2222 0.10249290229732627 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 4.6126418675664394E-5 0.0 0.0 0.0 0.0 11 4.6126418675664394E-5 0.0 0.0 0.0 0.0 12 4.6126418675664394E-5 4.6126418675664394E-5 0.0 0.0 9.225283735132879E-5 13 4.6126418675664394E-5 4.6126418675664394E-5 0.0 0.0 9.225283735132879E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1130 0.0 13.529779 1 TAGGACC 890 0.0 13.265763 4 TCCAACG 130 2.1827873E-10 12.35173 18 AGGACCT 1555 0.0 11.480047 5 CCAACGA 110 5.355141E-7 11.162794 19 GGCGAGG 1090 0.0 11.091895 19 GTATCAA 2415 0.0 11.089077 1 TGTAGGA 2395 0.0 10.973066 2 GGACCTG 1525 0.0 10.900717 6 GTCCTAA 985 0.0 10.753438 1 GTAGGAC 2495 0.0 10.751277 3 CGGTAGG 85 4.1395368E-4 10.580381 1 GGACGTG 2180 0.0 10.571717 6 AGGACGT 2275 0.0 10.545436 5 GTCCTAC 1880 0.0 10.524103 1 TTAGGAC 1430 0.0 10.435981 3 GACGTGA 1195 0.0 10.432977 7 GTCCTAT 260 0.0 10.376912 1 CTGTAGG 2360 0.0 10.373655 1 ATTTAGA 580 0.0 10.337154 1 >>END_MODULE