FastQCFastQC Report
Thu 26 May 2016
SRR1511795_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511795_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2167955
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65840.3036963405605744No Hit
GTCCTACAGTGGACATTTCTAAATT44280.20424778189584192No Hit
GGTATCAACGCAGAGTACTTTTTTT43540.20083442691384276No Hit
CTGTAGGACGTGGAATATGGCAAGA40100.1849669388894142No Hit
TATCAACGCAGAGTACTTTTTTTTT39090.1803081706031721No Hit
GTCCTAAAGTGTGTATTTCTCATTT33420.1541544912140704No Hit
CTTTAGGACGTGAAATATGGCGAGG31130.14359154133734325No Hit
GTCCTACAGTGTGCATTTCTCATTT25180.11614632222532295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13200.013.5828811
TAGGACC7300.012.493094
AAGATCG658.0259884E-411.6922515
GGCGAGG9600.011.27859419
TGTAGGA23400.011.1238782
TAGGACA7750.010.9096254
TATTCCG803.772137E-410.68744855
ATTCCGT909.54535E-510.5525836
GTAGGAC23450.010.5329973
AGGACCT14600.010.4759765
CTGTAGG23350.010.4558921
AGGACGT23700.010.34172255
GTCCTAC22350.010.2409831
GGACGTG22850.010.1831276
TTAGGAC15150.010.095663
TGCACCG856.595477E-410.0587745
GACGTGG11750.010.0227097
TGGCGAG20850.010.02162118
TGGACCG951.6445827E-49.9999515
GTCCTAA11050.09.838991
Produced by FastQC (version 0.11.5)