FastQCFastQC Report
Thu 26 May 2016
SRR1511794_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511794_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58148
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15392.6466946412602326No Hit
GGTATCAACGCAGAGTACTTTTTTT12382.1290500103184975No Hit
TATCAACGCAGAGTACTTTTTTTTT10221.757584095755658No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA7231.2433789640228383No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5360.9217857879892688No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4820.828919309348559No Hit
GTACTGGTTCACTATCGGTCAGTCA4030.6930590905964091No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3600.6191098576047328No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT3150.5417211254041412No Hit
GGATACCACGTGTCCCGCCCTACTC2720.46777189241246475No Hit
ATACAGGGTGACAGCCCCGTACACA2670.45917314439017676No Hit
GTGGTATCAACGCAGAGTACATGGG2340.40242140744307625No Hit
ATCATTAACTGAATCCATAGGTTAA2300.3955424090252459No Hit
GTGGTATCAACGCAGAGTACTTTTT2240.3852239113985004No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2170.37318566416729726No Hit
GGTATCAACGCAGAGTACATGGGGG2110.36286716654055173No Hit
GGTTAATGAGGCGAACCGGGGGAAC2040.35082891930934856No Hit
GTATTTAGCCTTGGAGGATGGTCCC2010.34566967049597574No Hit
GTACATGGGGGTTAAGCGACTAAGC1780.3061154295934512No Hit
GTTAATGATAGTGTGTCGAAACACA1730.29751668157116323No Hit
CCCATATTCAGACAGGATACCACGT1690.2906376831533329No Hit
ATCAACGCAGAGTACTTTTTTTTTT1620.2785994359221297No Hit
GTGGATGCCCTGGCAGTCAGAGGCG1600.27515993671321454No Hit
TATTCAGACAGGATACCACGTGTCC1570.2700006878998418No Hit
AGCGTACACGGTGGATGCCCTGGCA1520.26140193987755383No Hit
GCCTTGGAGGATGGTCCCCCCATAT1500.25796244066863866No Hit
GTTCACTATCGGTCAGTCAGGAGTA1450.24936369264635072No Hit
GTATCAACGCAGAGTACATGGGGGT1450.24936369264635072No Hit
ACTTAGATGTTTCAGTTCCCCCGGT1430.24592419343743552No Hit
ACCCTGTATCGCGCGCCTTTCCAGA1340.2304464469973172No Hit
GCCCAGAGCCTGAATCAGTGTGTGT1330.22872669739285958No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG1250.21496870055719886No Hit
CTATCGGTCAGTCAGGAGTATTTAG1170.20121070372153813No Hit
GGTTCACTATCGGTCAGTCAGGAGT1160.19949095411708057No Hit
GTCAGGAGTATTTAGCCTTGGAGGA1150.19777120451262295No Hit
GNATCAACGCAGAGTACTTTTTTTT1130.19433170530370777No Hit
GTACTTAGATGTTTCAGTTCCCCCG1120.1926119556992502No Hit
TCTAAGTACCCCGAGGAAAAGAAAT1120.1926119556992502No Hit
GTTATAACCGGCGATTTCCGAATGG1020.17541445965467428No Hit
CATCTAAGTACCCCGAGGAAAAGAA1010.1736947100502167No Hit
CACTATCATTAACTGAATCCATAGG1010.1736947100502167No Hit
GAGTACATGGGGGTTAAGCGACTAA1000.1719749604457591No Hit
GCCTGAATCAGTGTGTGTGTTAGTG1000.1719749604457591No Hit
GTGCTAATCTGCGATAAGCGTCGGT990.17025521084130152No Hit
GGAGTATTTAGCCTTGGAGGATGGT970.16681571163238632No Hit
CGGTACTGGTTCACTATCGGTCAGT910.1564972140056408No Hit
TATCAACGCAGAGTACATGGGGGTT900.1547774644011832No Hit
TCATTAACCTATGGATTCAGTTAAT890.1530577147967256No Hit
GGTATCAACGCAGAGTACATGGGGA890.1530577147967256No Hit
GAATCAGTGTGTGTGTTAGTGGAAG890.1530577147967256No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG880.15133796519226803No Hit
TCACGGTACTGGTTCACTATCGGTC860.14789846598335282No Hit
ACGCAGAGTACATGGGGGTTAAGCG860.14789846598335282No Hit
CACACACACTGATTCAGGCTCTGGG830.14273921716998003No Hit
GATATGAACCGTTATAACCGGCGAT820.14101946756552244No Hit
GCCCTACTCATCGAGCTCACAGCAT810.13929971796106486No Hit
GTCCCGCCCTACTCATCGAGCTCAC810.13929971796106486No Hit
GTGGTATCCTGTCTGAATATGGGGG780.1341404691476921No Hit
GGGTGACAGCCCCGTACACAAAAAT770.1324207195432345No Hit
CCTATGGATTCAGTTAATGATAGTG770.1324207195432345No Hit
GAATATGGGGGGACCATCCTCCAAG760.13070096993877692No Hit
GNTATCAACGCAGAGTACTTTTTTT760.13070096993877692No Hit
TTCCAGACGCTTCCACTAACACACA750.12898122033431933No Hit
CCCCAGTAGCGGCGAGCGAACGGGG750.12898122033431933No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT740.12726147072986171No Hit
AGCTCACAGCATGTGCATTTTTGTG730.12554172112540413No Hit
GGGTACTTAGATGTTTCAGTTCCCC700.12038247231203136No Hit
AACGCAGAGTACTTTTTTTTTTTTT690.11866272270757378No Hit
TAAGTACCCCGAGGAAAAGAAATCA690.11866272270757378No Hit
CATTAACCTATGGATTCAGTTAATG680.11694297310311619No Hit
GCGTACACGGTGGATGCCCTGGCAG680.11694297310311619No Hit
ATCCATAGGTTAATGAGGCGAACCG670.1152232234986586No Hit
GTCCCCCCATATTCAGACAGGATAC670.1152232234986586No Hit
GGTGATATGAACCGTTATAACCGGC650.11178372428974341No Hit
CCGTTATAACCGGCGATTTCCGAAT650.11178372428974341No Hit
TCGGTAAGGTGATATGAACCGTTAT650.11178372428974341No Hit
GGGTTTCCCCATTCGGAAATCGCCG640.11006397468528582No Hit
ATTTAGCCTTGGAGGATGGTCCCCC640.11006397468528582No Hit
CTATCATTAACTGAATCCATAGGTT630.10834422508082824No Hit
TCCCCCAGTAGCGGCGAGCGAACGG630.10834422508082824No Hit
GAGTAGGGCGGGACACGTGGTATCC610.10490472587191306No Hit
TTTGTGTACGGGGCTGTCACCCTGT610.10490472587191306No Hit
GTATCAACGCAGAGTACATGGGGAA610.10490472587191306No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA590.10146522666299787No Hit
CAACGCAGAGTACTTTTTTTTTTTT590.10146522666299787No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAAT500.00100610313.9821651
ACTAAGC500.001534251613.22409619
AGCGACT500.001534251613.22409615
GACTAAG500.001534251613.22409618
GGTATCA3550.012.6599081
GCAGTCA604.185025E-412.594377513
TGAGGCG604.185025E-412.59437757
AGTCAGA604.185025E-412.594377515
TAATGAG701.1144869E-412.1445794
TGGCAGT550.003139533312.02190611
GCGACTA550.003139533312.02190616
TAAGCGA550.003139533312.02190613
AGGCGAA550.003139533312.0219069
CTGGCAG550.003139533312.02190610
AAGCGAC550.003139533312.02190614
AGGTTAA550.003139533312.02190619
CGACTAA550.003139533312.02190617
TAGGTTA550.003139533312.02190618
GGCAGTC658.2062563E-411.62557912
ACCGGGG600.006011915411.02008115
Produced by FastQC (version 0.11.5)