FastQCFastQC Report
Thu 26 May 2016
SRR1511794_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511794_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58148
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19023.2709637476783384No Hit
GGTATCAACGCAGAGTACTTTTTTT16692.8702620898397195No Hit
TATCAACGCAGAGTACTTTTTTTTT15492.6638921373048086No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8041.382678681983903No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA6351.0920409988305702No Hit
GTACTTTTTTTTTTTTTTTTTTTTT5380.925225287198184No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4670.803123065281695No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT4380.7532503267524249No Hit
ATCATTAACTGAATCCATAGGTTAA3290.5657976198665474No Hit
GTGGTATCAACGCAGAGTACATGGG2810.4832496388525831No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2780.47809039003921033No Hit
GTGGTATCAACGCAGAGTACTTTTT2610.4488546467634313No Hit
ATACAGGGTGACAGCCCCGTACACA2530.43509664992777053No Hit
GTACTGGTTCACTATCGGTCAGTCA2510.43165715071885535No Hit
GGTATCAACGCAGAGTACATGGGGG2250.38694366100295796No Hit
ATCAACGCAGAGTACTTTTTTTTTT2200.37834491298067No Hit
GGATACCACGTGTCCCGCCCTACTC2140.36802641535392444No Hit
GGTTAATGAGGCGAACCGGGGGAAC2110.36286716654055173No Hit
GTGGATGCCCTGGCAGTCAGAGGCG1720.29579693196670565No Hit
TCTAAGTACCCCGAGGAAAAGAAAT1680.28891793354887524No Hit
GTATCAACGCAGAGTACATGGGGGT1640.2820389351310449No Hit
GTACATGGGGGTTAAGCGACTAAGC1580.27172043750429936No Hit
GTTAATGATAGTGTGTCGAAACACA1580.27172043750429936No Hit
TATTCAGACAGGATACCACGTGTCC1500.25796244066863866No Hit
AGCGTACACGGTGGATGCCCTGGCA1380.23732544541514755No Hit
ACTTAGATGTTTCAGTTCCCCCGGT1370.23560569581069No Hit
CCCATATTCAGACAGGATACCACGT1290.2218476989750292No Hit
GTTATAACCGGCGATTTCCGAATGG1220.20980945174382612No Hit
CATCTAAGTACCCCGAGGAAAAGAA1200.20636995253491092No Hit
GGTATCAACGCAGAGTACATGGGGA1150.19777120451262295No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG1100.189172456490335No Hit
TATCAACGCAGAGTACATGGGGGTT1080.1857329572814198No Hit
ACCCTGTATCGCGCGCCTTTCCAGA1070.18401320767696222No Hit
GTATTTAGCCTTGGAGGATGGTCCC1050.18057370846804705No Hit
ACGCAGAGTACATGGGGGTTAAGCG1040.17885395886358946No Hit
GGTGATATGAACCGTTATAACCGGC1030.17713420925913187No Hit
CACTATCATTAACTGAATCCATAGG980.16853546123684393No Hit
GCCCAGAGCCTGAATCAGTGTGTGT980.16853546123684393No Hit
CACACACACTGATTCAGGCTCTGGG980.16853546123684393No Hit
GATATGAACCGTTATAACCGGCGAT970.16681571163238632No Hit
AACGCAGAGTACTTTTTTTTTTTTT960.16509596202792873No Hit
TAAGTACCCCGAGGAAAAGAAATCA960.16509596202792873No Hit
CAACGCAGAGTACTTTTTTTTTTTT910.1564972140056408No Hit
GAGTACATGGGGGTTAAGCGACTAA890.1530577147967256No Hit
GCCTGAATCAGTGTGTGTGTTAGTG880.15133796519226803No Hit
ATCCATAGGTTAATGAGGCGAACCG870.1496182155878104No Hit
GTTCACTATCGGTCAGTCAGGAGTA870.1496182155878104No Hit
GTGCTAATCTGCGATAAGCGTCGGT870.1496182155878104No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT870.1496182155878104No Hit
CTATCGGTCAGTCAGGAGTATTTAG860.14789846598335282No Hit
CGGTACTGGTTCACTATCGGTCAGT850.14617871637889523No Hit
CCTATGGATTCAGTTAATGATAGTG850.14617871637889523No Hit
CTATCATTAACTGAATCCATAGGTT840.14445896677443762No Hit
GTCAGGAGTATTTAGCCTTGGAGGA830.14273921716998003No Hit
TCATTAACCTATGGATTCAGTTAAT820.14101946756552244No Hit
TCAACGCAGAGTACTTTTTTTTTTT820.14101946756552244No Hit
GGGTACTTAGATGTTTCAGTTCCCC820.14101946756552244No Hit
GGAGTATTTAGCCTTGGAGGATGGT790.13586021875214968No Hit
GTACTTAGATGTTTCAGTTCCCCCG790.13586021875214968No Hit
GAATCAGTGTGTGTGTTAGTGGAAG790.13586021875214968No Hit
GGTTCACTATCGGTCAGTCAGGAGT770.1324207195432345No Hit
GGGTTTCCCCATTCGGAAATCGCCG750.12898122033431933No Hit
TCGGTAAGGTGATATGAACCGTTAT750.12898122033431933No Hit
ATGATAGTGTGTCGAAACACACTGG750.12898122033431933No Hit
TCACGGTACTGGTTCACTATCGGTC740.12726147072986171No Hit
GGGAAACCCAGTGTGTTTCGACACA740.12726147072986171No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT710.12210222191648895No Hit
GCCTTGGAGGATGGTCCCCCCATAT690.11866272270757378No Hit
CAGTAGCGGCGAGCGAACGGGGAGC680.11694297310311619No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG680.11694297310311619No Hit
GCGTACACGGTGGATGCCCTGGCAG660.11350347389420101No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA660.11350347389420101No Hit
CCGTTATAACCGGCGATTTCCGAAT650.11178372428974341No Hit
CATTAACTGAATCCATAGGTTAATG650.11178372428974341No Hit
TTTGTGTACGGGGCTGTCACCCTGT640.11006397468528582No Hit
GTGGTATCCTGTCTGAATATGGGGG630.10834422508082824No Hit
GATTCAGGCTCTGGGCTGCTCCCCG630.10834422508082824No Hit
GAATATGGGGGGACCATCCTCCAAG610.10490472587191306No Hit
AGCTCACAGCATGTGCATTTTTGTG610.10490472587191306No Hit
GGTATCAACGCAGAGTACATGGGCA610.10490472587191306No Hit
GGGTGACAGCCCCGTACACAAAAAT610.10490472587191306No Hit
GGGGAAACCCAGTGTGTTTCGACAC610.10490472587191306No Hit
CCCCAGTAGCGGCGAGCGAACGGGG600.10318497626745546No Hit
AGGTTAATGAGGCGAACCGGGGGAA590.10146522666299787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3750.013.4816371
GCCCTAC550.003012889312.08817117
CGCCCTA550.003012889312.08817116
CCGCCCT550.003012889312.08817115
CCCGCCC550.003012889312.08817114
CCCTACT550.003012889312.08817118
GTGGTAT1251.5841579E-811.44667151
TGGTATC1251.6891136E-811.3974182
CCTACTC600.00577085811.08082419
TGGCAGT600.00577085811.08082411
CAGTCAG803.6620517E-410.68507914
GTATCAA6900.09.2624041
GCCCTGG850.0072704218.9391527
TATCAAC9250.06.87952332
ATCAACG9650.06.59436133
TCAACGC9800.06.4934274
CAACGCA9850.06.4604655
AACGCAG10100.06.3005536
CGCAGAG11500.05.5335298
ACGCAGA11550.05.50957447
Produced by FastQC (version 0.11.5)