FastQCFastQC Report
Thu 26 May 2016
SRR1511793_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511793_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566514
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49590.3165627629245574No Hit
GGTATCAACGCAGAGTACTTTTTTT35220.2248304196451484No Hit
TATCAACGCAGAGTACTTTTTTTTT33170.2117440380360469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10600.013.7632821
GTGCGCC658.422401E-411.62485511
TACGGTG600.006135641611.0190417
GTATTAC1301.9155414E-710.7611761
CGACTAA1554.0236046E-910.35957617
AGGACCG1201.6405793E-610.2322945
CTAGACT750.002777722410.0748744
CTAATTG750.002777722410.0748743
TATAGTG1701.722583E-910.0007945
GTATCAA21300.09.9925191
GTGGTAT2650.09.8039821
TTATAGT1356.97004E-79.7950164
TAAGCGA1356.97004E-79.79501613
AAGCGAC1752.8412614E-99.71505714
GGATAAT1156.393299E-59.55806351
GCGACTA1401.1443008E-69.44549716
CCTAGAC800.00473614229.4451943
TGGACCG800.00473614229.4451945
ATAAGAC1107.281189E-59.4451943
TATACTG2750.09.2734645
Produced by FastQC (version 0.11.5)