FastQCFastQC Report
Thu 26 May 2016
SRR1511793_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511793_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566514
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61020.38952731989627926No Hit
GGTATCAACGCAGAGTACTTTTTTT44880.28649600322754853No Hit
TATCAACGCAGAGTACTTTTTTTTT43640.2785803382542384No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20560.1312468321381105No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17690.1129258978853684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10900.013.9973581
GGCGTCT550.003074881212.0875378
CGCCCTA1751.8189894E-1211.39682116
ATCGCGC1104.9856135E-711.2241418
AGCGTAC855.1231225E-511.2184721
TATCGCG1053.4772656E-610.8541157
TCGCGCG1053.4772656E-610.8541159
TCTACGT803.7704164E-410.687593
TAGCACT1409.6828444E-810.1786584
GTTCTAG1502.3052053E-810.1714141
CTACGTT750.00264740610.1334194
GTATAAG1605.798938E-910.1316811
TCTATAC1606.166374E-910.0938353
GTATCAA22500.09.9595091
GTGCTAG1553.8317012E-89.8433041
CGCGCGC1451.6228114E-79.82484610
GTATAAT1752.410161E-99.8081491
GTGGTAT3450.09.67391
GTATTGG1606.257687E-89.53571
GTCCTAT1201.6260283E-59.53571
Produced by FastQC (version 0.11.5)