##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511793_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1566514 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.38699813726529 33.0 31.0 34.0 28.0 34.0 2 31.56050568331978 33.0 31.0 34.0 28.0 34.0 3 31.6750357800824 34.0 31.0 34.0 28.0 34.0 4 35.23271352825446 37.0 35.0 37.0 32.0 37.0 5 34.91396182862075 37.0 35.0 37.0 32.0 37.0 6 34.86556838943029 37.0 35.0 37.0 32.0 37.0 7 34.7297617512515 37.0 35.0 37.0 32.0 37.0 8 34.66920180732505 37.0 35.0 37.0 32.0 37.0 9 36.2488557395593 39.0 37.0 39.0 32.0 39.0 10 36.096139581261326 39.0 37.0 39.0 32.0 39.0 11 36.079655847314484 39.0 37.0 39.0 31.0 39.0 12 35.92937630943611 39.0 37.0 39.0 30.0 39.0 13 35.92056055675213 39.0 37.0 39.0 30.0 39.0 14 37.05143203316408 40.0 37.0 41.0 31.0 41.0 15 36.99074505558201 40.0 37.0 41.0 31.0 41.0 16 36.899968337340106 40.0 37.0 41.0 31.0 41.0 17 36.846841458167624 40.0 37.0 41.0 31.0 41.0 18 36.78983973331869 40.0 37.0 41.0 31.0 41.0 19 36.804218794086744 40.0 37.0 41.0 30.0 41.0 20 36.681322988495474 40.0 37.0 41.0 30.0 41.0 21 36.590878217494385 40.0 37.0 41.0 30.0 41.0 22 36.668628559974564 40.0 37.0 41.0 30.0 41.0 23 36.5725943081262 40.0 37.0 41.0 30.0 41.0 24 36.45542714587932 40.0 37.0 41.0 30.0 41.0 25 36.04207814293393 39.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20495858637616138 1101 2 0.16937416173370679 1101 3 0.15591058204790897 1101 4 0.188802888138909 1101 5 0.1795213918773797 1101 6 0.25374730587418526 1101 7 0.26240033498100246 1101 8 0.2213778667895383 1101 9 0.2681991200266367 1101 10 0.41046644053336223 1101 11 0.12838080187344048 1101 12 0.27325665472672256 1101 13 0.2866554802465231 1101 14 0.27382342625289624 1101 15 0.1309499830617824 1101 16 0.17218211019855545 1101 17 0.3178422547969433 1101 18 0.2421304328051903 1101 19 0.39545647683865326 1101 20 0.3309176346822085 1101 21 0.3397722248022461 1101 22 -0.01936608512450988 1101 23 0.0771403313386756 1101 24 0.004385923005727932 1101 25 0.11384832691732072 1102 1 0.41814723343108895 1102 2 0.40888088670554623 1102 3 0.46826150631743957 1102 4 0.3809200947910014 1102 5 0.44159445554927856 1102 6 0.6128769520677864 1102 7 0.5748920529959776 1102 8 0.6205115711558022 1102 9 0.6496507863620238 1102 10 0.6448010658563632 1102 11 0.8188726689641399 1102 12 0.875501900867313 1102 13 0.8681005209938846 1102 14 1.0540671650722189 1102 15 0.970749476204297 1102 16 1.0037803676689094 1102 17 0.9260029087424826 1102 18 0.9648537151214924 1102 19 0.986481703765044 1102 20 1.012765179901848 1102 21 1.0260775484622258 1102 22 0.8741876318303738 1102 23 1.0862091049423341 1102 24 1.0493322557427618 1102 25 1.159707748503486 1103 1 0.4304586320898771 1103 2 0.493997646947399 1103 3 0.468211104015424 1103 4 0.4597834057844068 1103 5 0.5145276047869274 1103 6 0.5225621362347468 1103 7 0.49182396814090623 1103 8 0.46236187530060135 1103 9 0.6381020407174915 1103 10 0.651968884139265 1103 11 0.759402025504933 1103 12 0.8564462669081792 1103 13 0.7639357635053301 1103 14 0.8260196586115569 1103 15 1.018824108267495 1103 16 0.7314073458319825 1103 17 0.7453854958162793 1103 18 0.81917986179932 1103 19 1.0496836449155182 1103 20 0.9898122770347229 1103 21 0.666183220197631 1103 22 0.9907849571482146 1103 23 1.0852545158891047 1103 24 1.0185135390770057 1103 25 0.631051748796736 1104 1 0.355336453229004 1104 2 0.3069449804218145 1104 3 0.34102521603767144 1104 4 0.24774690726998472 1104 5 0.34062738719090646 1104 6 0.3155366112491862 1104 7 0.326382913182492 1104 8 0.3384293216822485 1104 9 0.48292218485081406 1104 10 0.4079135572297261 1104 11 0.5488898337639156 1104 12 0.562823838189253 1104 13 0.6055321714690152 1104 14 0.759620066277293 1104 15 0.8456233945564762 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1.1084641886762938 1107 16 1.1904509067666282 1107 17 1.242043071384586 1107 18 1.2150127613970483 1107 19 1.3071093141984846 1107 20 1.2369291496968842 1107 21 1.1937662603256953 1107 22 1.1136864286107198 1107 23 1.2782222158874816 1107 24 1.2691729328649899 1107 25 1.0491775020509095 1108 1 0.3583774909391586 1108 2 0.3695942175245328 1108 3 0.30038768757351164 1108 4 0.27059029039656224 1108 5 0.24807518449738097 1108 6 0.37631232892765354 1108 7 0.3534515801571203 1108 8 0.31305874614548657 1108 9 0.3609152372162825 1108 10 0.4128937390142937 1108 11 0.39760271133619796 1108 12 0.5635143982334441 1108 13 0.4217278204260353 1108 14 0.6622566759963462 1108 15 0.7584978786201972 1108 16 0.6427139658939893 1108 17 0.5034014096959893 1108 18 0.6315451711230793 1108 19 0.7402417146723508 1108 20 0.6986294157971571 1108 21 0.7489978560624593 1108 22 0.7623753366743884 1108 23 1.005882363801426 1108 24 1.0137391974648011 1108 25 0.9621810158617734 1109 1 -0.045805771187350075 1109 2 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-0.8862467171919448 2314 24 -0.8014386239670301 2314 25 -0.7868665774920629 2315 1 -0.60983645569004 2315 2 -0.6320650902593883 2315 3 -0.5923068089902159 2315 4 -0.6705003559185485 2315 5 -0.6380864831363979 2315 6 -0.6811239390895025 2315 7 -0.7884503269340755 2315 8 -0.8102988418820942 2315 9 -0.8724593660798803 2315 10 -0.9206281073927158 2315 11 -0.9632528761945807 2315 12 -0.8747748979876846 2315 13 -0.9007656838912723 2315 14 -1.0997992423486949 2315 15 -1.3027547972999756 2315 16 -1.1326409606955465 2315 17 -1.2484613842052212 2315 18 -1.1647016514006978 2315 19 -1.20705808100697 2315 20 -1.032521813252444 2315 21 -1.2320981445475923 2315 22 -1.3858682680359564 2315 23 -1.2738690738562255 2315 24 -1.3222195731550386 2315 25 -1.29822557672842 2316 1 -1.5637962485123929 2316 2 -1.1863104584857993 2316 3 -1.0388417507858208 2316 4 -0.7351382712673669 2316 5 -0.8252878113329913 2316 6 -0.6935448543540161 2316 7 -1.02434179623787 2316 8 -0.7790995784553445 2316 9 -1.1137087269974941 2316 10 -1.0152243441690487 2316 11 -0.9692038691123983 2316 12 -0.9927317173663042 2316 13 -1.1669537640603949 2316 14 -1.4464525854475312 2316 15 -1.1979961925413747 2316 16 -1.2591171452286076 2316 17 -1.254812522072946 2316 18 -1.3771613768935964 2316 19 -1.372993155709814 2316 20 -1.3809169637802938 2316 21 -1.5096889873043082 2316 22 -1.2570204993777594 2316 23 -1.4112498925166648 2316 24 -1.4839317775212777 2316 25 -1.5391834503877178 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16715.0 3 0.0 4 283.0 5 2934.0 6 3662.0 7 2938.0 8 2397.0 9 2190.0 10 2179.0 11 1962.0 12 1946.0 13 1798.0 14 1868.0 15 1824.0 16 1977.0 17 2006.0 18 2261.0 19 2432.0 20 3190.0 21 3872.0 22 4988.0 23 6358.0 24 7703.0 25 9271.0 26 10743.0 27 12383.0 28 14880.0 29 18223.0 30 23345.0 31 30120.0 32 39485.0 33 51964.0 34 68817.0 35 93125.0 36 137867.0 37 252122.0 38 726686.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.69888602135649 18.57981505057071 12.69915140699259 25.022147521080207 2 16.321909666942013 20.55155587501931 38.111309570166625 25.01522488787205 3 18.770339748000975 24.471150592972677 29.82718315955044 26.931326499475905 4 13.442139680845495 16.800041365733087 36.53679443656424 33.22102451685717 5 14.193456521864059 36.853692814484816 34.85759931141447 14.095251352236657 6 32.79970686505196 36.02457430958166 17.439039804304336 13.736679021062054 7 28.335846344175668 31.503261381641018 21.799741336496194 18.361150937687118 8 26.489070637096123 34.43741964642512 20.371602168892203 18.70190754758655 9 26.850127097491626 14.793292622983262 19.645148399567447 38.711431879957665 10 15.947958332960956 28.169234363689057 33.35891029381161 22.523897009538377 11 35.040542248585076 22.295044921398723 23.18274844655075 19.481664383465453 12 23.06082167155863 24.67714939030229 30.57802228387362 21.68400665426546 13 29.26497943842187 21.11024861571617 25.421668749848394 24.20310319601357 14 22.3280481374568 20.705400653936064 27.178180341829055 29.78837086677808 15 24.32988150760223 27.921742161257416 23.671732266676198 24.076644064464155 16 23.888549848325454 27.231071322785912 25.35180068840839 23.528578140480253 17 23.00905066919287 26.958009950756907 26.6586190739438 23.374320306106426 18 22.960898455229486 26.659563833257476 27.38525296024094 22.994284751272094 19 23.852196660866102 26.486836376821397 27.317279002932626 22.34368795937987 20 24.10332751574515 25.852689474846695 27.318045035026817 22.72593797438133 21 24.320306106424837 25.74046577304767 27.04393321732203 22.895294903205464 22 24.18988914238877 25.652308246207824 27.003971876408382 23.153830734995026 23 24.02602211023968 25.6893331307604 26.941157244684693 23.343487514315225 24 23.64805080558361 25.848706814551704 27.088076034733493 23.415166345131194 25 23.82741008521485 25.787689968343663 27.078009666066823 23.306890280374667 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 613.0 1 613.0 2 618.5 3 624.0 4 624.0 5 624.0 6 1533.5 7 2443.0 8 2443.0 9 2443.0 10 3088.5 11 3734.0 12 3734.0 13 3734.0 14 5202.0 15 6670.0 16 6670.0 17 6670.0 18 11758.5 19 16847.0 20 16847.0 21 16847.0 22 24903.5 23 32960.0 24 32960.0 25 32960.0 26 45850.0 27 58740.0 28 58740.0 29 58740.0 30 73114.5 31 87489.0 32 87489.0 33 87489.0 34 102438.0 35 117387.0 36 117387.0 37 117387.0 38 133113.0 39 148839.0 40 148839.0 41 148839.0 42 164291.5 43 179744.0 44 179744.0 45 179744.0 46 192600.0 47 205456.0 48 205456.0 49 205456.0 50 206866.5 51 208277.0 52 208277.0 53 208277.0 54 195003.0 55 181729.0 56 181729.0 57 181729.0 58 159141.5 59 136554.0 60 136554.0 61 136554.0 62 114532.0 63 92510.0 64 92510.0 65 92510.0 66 71026.0 67 49542.0 68 49542.0 69 49542.0 70 36257.5 71 22973.0 72 22973.0 73 22973.0 74 15932.5 75 8892.0 76 8892.0 77 8892.0 78 6054.5 79 3217.0 80 3217.0 81 3217.0 82 2083.0 83 949.0 84 949.0 85 949.0 86 608.5 87 268.0 88 268.0 89 268.0 90 154.5 91 41.0 92 41.0 93 41.0 94 28.5 95 16.0 96 16.0 97 16.0 98 8.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4162107711772764 2 0.0 3 0.0 4 0.0 5 0.02585358317895659 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.2767201569855105E-4 17 0.0 18 1.9150802354782657E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004596192565147837 25 1.9150802354782657E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1566514.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.63833683655263 #Duplication Level Percentage of deduplicated Percentage of total 1 76.57817588372788 51.0304227886863 2 14.828497146653238 19.76292775277076 3 4.602118624577626 9.200325931991282 4 1.7996729473440491 4.797088482429768 5 0.8125249832662603 2.707265676150567 6 0.4354488555560759 1.7410552506982286 7 0.26513508691848314 1.2367712860483777 8 0.14890951353306078 0.7938465856786637 9 0.10364678622019408 0.621616450695071 >10 0.3970443672995248 4.241784174610301 >50 0.017619286836613193 0.8029336003022478 >100 0.009674147935253624 1.3035063677108065 >500 8.619581991020368E-4 0.4020762256755288 >1k 5.746387994013579E-4 0.9689398822549014 >5k 9.577313323355965E-5 0.38943954429715505 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6102 0.38952731989627926 No Hit GGTATCAACGCAGAGTACTTTTTTT 4488 0.28649600322754853 No Hit TATCAACGCAGAGTACTTTTTTTTT 4364 0.2785803382542384 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2056 0.1312468321381105 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1769 0.1129258978853684 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1090 0.0 13.997358 1 GGCGTCT 55 0.0030748812 12.087537 8 CGCCCTA 175 1.8189894E-12 11.396821 16 ATCGCGC 110 4.9856135E-7 11.224141 8 AGCGTAC 85 5.1231225E-5 11.218472 1 TATCGCG 105 3.4772656E-6 10.854115 7 TCGCGCG 105 3.4772656E-6 10.854115 9 TCTACGT 80 3.7704164E-4 10.68759 3 TAGCACT 140 9.6828444E-8 10.178658 4 GTTCTAG 150 2.3052053E-8 10.171414 1 CTACGTT 75 0.002647406 10.133419 4 GTATAAG 160 5.798938E-9 10.131681 1 TCTATAC 160 6.166374E-9 10.093835 3 GTATCAA 2250 0.0 9.959509 1 GTGCTAG 155 3.8317012E-8 9.843304 1 CGCGCGC 145 1.6228114E-7 9.824846 10 GTATAAT 175 2.410161E-9 9.808149 1 GTGGTAT 345 0.0 9.6739 1 GTATTGG 160 6.257687E-8 9.5357 1 GTCCTAT 120 1.6260283E-5 9.5357 1 >>END_MODULE