FastQCFastQC Report
Thu 26 May 2016
SRR1511792_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511792_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392668
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT66630.278475743396075No Hit
CTGTAGGACGTGGAATATGGCAAGA64700.27040943415467583No Hit
GTATCAACGCAGAGTACTTTTTTTT61070.2552380856851013No Hit
GTCCTAAAGTGTGTATTTCTCATTT57610.2407772411383443No Hit
CTTTAGGACGTGAAATATGGCGAGG50840.21248246727084574No Hit
GGTATCAACGCAGAGTACTTTTTTT38280.1599887656791498No Hit
TATCAACGCAGAGTACTTTTTTTTT37070.15493164952262495No Hit
GTCCTACAGTGTGCATTTCTCATTT34660.1448592115579763No Hit
CTGAAGGACCTGGAATATGGCGAGA28420.11877953815573243No Hit
CTGTAGGACCTGGAATATGGCGAGA25300.10573970145461051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC1059.822543E-1114.39209210
GGTATCA11500.013.9920281
TCCAACG2850.013.58783918
GTCAACG450.00823149813.3257412
ACCGCAA500.001567266113.2227358
GCGTGCG1001.0939402E-813.2227359
CCAACGA2100.012.59360619
TAGGACC11800.012.4868824
GGCGAGG14950.012.38301219
CGGTAGG1301.1768861E-912.3006841
GTACCGG550.00320563612.020926
CGTTAGG853.1733543E-511.7580071
GGCCTAC1703.6379788E-1211.7580071
CGACTGT600.004026228611.6600241
GCGACTA658.4251736E-411.62486716
CTACCGT907.961506E-611.5441394
CACCTTT7450.011.15651814
GTATCAA25900.011.0361831
CGACTAA700.001565649310.79451917
AGGACCT23650.010.783115
Produced by FastQC (version 0.11.5)