##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511792_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2392668 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.414994056843657 31.0 30.0 34.0 25.0 34.0 2 26.66743526473376 31.0 26.0 34.0 10.0 34.0 3 28.408075002465868 31.0 28.0 34.0 16.0 34.0 4 32.77425827569893 35.0 33.0 37.0 26.0 37.0 5 33.149101755864166 35.0 35.0 37.0 27.0 37.0 6 33.35398308499131 37.0 35.0 37.0 28.0 37.0 7 33.334995912512724 37.0 35.0 37.0 27.0 37.0 8 33.35326547602927 37.0 35.0 37.0 27.0 37.0 9 34.89695812373468 39.0 35.0 39.0 28.0 39.0 10 34.76496822793634 39.0 35.0 39.0 27.0 39.0 11 34.76055808829307 39.0 35.0 39.0 27.0 39.0 12 34.640462446106184 39.0 35.0 39.0 27.0 39.0 13 34.63745199919086 39.0 35.0 39.0 27.0 39.0 14 35.714827548159626 40.0 36.0 41.0 27.0 41.0 15 35.663983887442804 40.0 36.0 41.0 27.0 41.0 16 35.61680391930682 40.0 36.0 41.0 27.0 41.0 17 35.57954634742472 40.0 36.0 41.0 27.0 41.0 18 35.54750136667519 40.0 36.0 41.0 27.0 41.0 19 35.573174381067496 40.0 36.0 41.0 27.0 41.0 20 35.51307912338862 40.0 36.0 41.0 26.0 41.0 21 35.39631950609111 40.0 36.0 41.0 26.0 41.0 22 35.29865948806938 40.0 36.0 41.0 25.0 41.0 23 35.187718062012785 40.0 36.0 41.0 25.0 41.0 24 35.07731870865494 40.0 36.0 41.0 24.0 41.0 25 34.96828770226375 40.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8258704536828283 1101 2 3.4081023863129403 1101 3 1.996109693756857 1101 4 1.0710309296778675 1101 5 0.4415993373847158 1101 6 0.5731733268175319 1101 7 0.4743201911228141 1101 8 0.4661538227892379 1101 9 0.5202116016959977 1101 10 0.6045790650476377 1101 11 0.590221860646345 1101 12 0.4094932302260901 1101 13 0.34567354880588397 1101 14 0.37103434163243065 1101 15 0.726266266809958 1101 16 0.6605160710156923 1101 17 0.693126767777656 1101 18 0.7475638968409868 1101 19 0.8943146870979461 1101 20 0.8320983133339439 1101 21 0.7039087589654969 1101 22 0.7250193689650359 1101 23 0.646660587906787 1101 24 0.830914507622289 1101 25 0.7606468549357857 1102 1 1.4361892392615765 1102 2 4.009760830335711 1102 3 2.423919940436175 1102 4 1.4961390890706525 1102 5 1.2115613867205752 1102 6 0.9855945791894456 1102 7 0.9054435307812554 1102 8 0.7928559100757653 1102 9 0.8914943341438217 1102 10 0.8416151181785665 1102 11 0.738498900494541 1102 12 0.9574704598276043 1102 13 1.1261327518419364 1102 14 1.1415542657311022 1102 15 1.0317842933754164 1102 16 1.213969581452119 1102 17 1.1713430865632333 1102 18 1.0901369518694537 1102 19 1.230246375902496 1102 20 1.2984133038462815 1102 21 1.197164925948421 1102 22 1.2367613044489048 1102 23 1.3749376277549814 1102 24 1.1491687770720063 1102 25 1.0892920162261035 1103 1 1.2479630617056827 1103 2 3.892974635414941 1103 3 2.42119126667885 1103 4 1.63319964908802 1103 5 1.2270117882834128 1103 6 1.0073959683798108 1103 7 0.9754930206879209 1103 8 0.7691578419186129 1103 9 0.9550267847624454 1103 10 1.010594280323133 1103 11 1.3543395777248435 1103 12 1.0513452156339866 1103 13 1.1617021028295653 1103 14 1.493111895425045 1103 15 1.354754121897237 1103 16 1.121833701269793 1103 17 1.155563837594272 1103 18 1.3281495414071145 1103 19 1.4785964952860482 1103 20 1.459408962626398 1103 21 1.500961105674925 1103 22 1.5744359300186872 1103 23 1.5164056112583495 1103 24 1.7151509780704828 1103 25 1.6692711783706713 1104 1 1.1683378295388103 1104 2 3.700671901306155 1104 3 2.1800006755339503 1104 4 1.3683508223621033 1104 5 1.097337454156893 1104 6 0.8981537561312507 1104 7 0.794068259177287 1104 8 0.8966527633965313 1104 9 0.9235896245964454 1104 10 1.0439760176785526 1104 11 0.9811831592447717 1104 12 1.0763110577490167 1104 13 1.0949899470564688 1104 14 1.2586052880091714 1104 15 1.1394547559529755 1104 16 1.1605901736142172 1104 17 1.1491372696570323 1104 18 1.1145765445289442 1104 19 0.9763017176653719 1104 20 1.04309311221823 1104 21 0.9285884710674068 1104 22 0.997088642860902 1104 23 1.1090504579337832 1104 24 1.0015303572180798 1104 25 1.07998936432422 1105 1 1.213863285224047 1105 2 3.8894433097791037 1105 3 2.272121711467168 1105 4 1.4764441702429068 1105 5 0.9958108069187617 1105 6 0.9431923021495976 1105 7 0.7071106191219698 1105 8 0.6679248537822815 1105 9 0.9097593562817039 1105 10 0.7756205999411634 1105 11 0.9170983101508767 1105 12 0.701924181101063 1105 13 0.8605375589260618 1105 14 0.9936624251238939 1105 15 1.0140324485514682 1105 16 0.9149436484982942 1105 17 0.9031467972948377 1105 18 1.030269990030952 1105 19 0.9804679656136557 1105 20 1.0697116382337981 1105 21 1.1399987863743135 1105 22 1.0985435098041663 1105 23 1.108393668235692 1105 24 1.0854475036163862 1105 25 1.1981863868775946 1106 1 1.0553153881586361 1106 2 3.707319653865124 1106 3 2.0407499036714682 1106 4 1.086762480614773 1106 5 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0.5207558585213334 1107 18 0.5044546392183662 1107 19 0.660302379125703 1107 20 0.5423021032016422 1107 21 0.5138556995181247 1107 22 0.6895884599686468 1107 23 0.7015867010829453 1107 24 0.7610916215522607 1107 25 0.7718671572414166 1108 1 0.7592006946243295 1108 2 3.4467917163137685 1108 3 1.761626354472618 1108 4 1.058286897084578 1108 5 0.5727852254754708 1108 6 0.38894070515010526 1108 7 0.4192593749496538 1108 8 0.360974040483228 1108 9 0.4146751548698049 1108 10 0.2512687505435949 1108 11 0.34883560115672907 1108 12 0.29745847278135074 1108 13 0.3058000146385922 1108 14 0.5223388372425362 1108 15 0.38728451088231 1108 16 0.3920864551531196 1108 17 0.2888383739139968 1108 18 0.34095854788665747 1108 19 0.33500001310100913 1108 20 0.36382313515760245 1108 21 0.38333284126574085 1108 22 0.3898950470174185 1108 23 0.427870877307889 1108 24 0.4708353631805622 1108 25 0.39197112106442233 1109 1 0.6066752761005567 1109 2 2.5739105629560335 1109 3 1.4182491145300524 1109 4 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2314 8 -0.3534785118166752 2314 9 -0.23254468863324718 2314 10 -0.28714119615320755 2314 11 -0.3036752965208791 2314 12 -0.3895996075528245 2314 13 -0.33519650283955826 2314 14 -1.1046617650241402 2314 15 -0.774960529079209 2314 16 -0.6988021104994289 2314 17 -0.6155057415133243 2314 18 -0.3402333511357938 2314 19 -0.48762754415041343 2314 20 -0.36550650103983173 2314 21 -0.2964016070597211 2314 22 -0.4384445158975083 2314 23 -0.45735603517751144 2314 24 -0.47210607721265063 2314 25 -0.3889175658498729 2315 1 -4.202697449767303 2315 2 -13.519992585356016 2315 3 -6.981712392051758 2315 4 -3.057200352964852 2315 5 -1.9218534868783763 2315 6 -1.273192461635226 2315 7 -1.1955176080553613 2315 8 -1.1923014162761945 2315 9 -1.127390882251504 2315 10 -1.1515806602718968 2315 11 -1.0937933354540803 2315 12 -1.0605869728812252 2315 13 -1.0923240918134667 2315 14 -1.067595370201154 2315 15 -1.1674091943117304 2315 16 -1.092631089414276 2315 17 -1.0626403872157084 2315 18 -1.0909326709727267 2315 19 -1.0118557266909747 2315 20 -1.0277342867615928 2315 21 -1.2362437298643911 2315 22 -1.1400538800393534 2315 23 -1.170177973979449 2315 24 -1.3821885001921714 2315 25 -1.515678449289723 2316 1 -1.3979310077322467 2316 2 -1.132804655527142 2316 3 -0.7579271508865411 2316 4 -0.9521732281214135 2316 5 -1.0925968843226457 2316 6 -1.305170289188844 2316 7 -1.262537294698106 2316 8 -1.3660560171260556 2316 9 -1.3078080707960567 2316 10 -1.5194892042133716 2316 11 -1.7248191040553422 2316 12 -1.3811739990609198 2316 13 -1.3353685481255653 2316 14 -1.4976158675155702 2316 15 -1.9824204515059591 2316 16 -1.8992234637217464 2316 17 -2.0124666306938366 2316 18 -1.763352160902727 2316 19 -1.8860392919558038 2316 20 -2.0443549769466998 2316 21 -2.1597682882212226 2316 22 -2.03077216971424 2316 23 -1.8952450176788815 2316 24 -1.736607141030042 2316 25 -2.0251769470498147 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 92692.0 3 1.0 4 802.0 5 4275.0 6 5195.0 7 4580.0 8 4201.0 9 4012.0 10 3843.0 11 3676.0 12 3783.0 13 3700.0 14 3617.0 15 3733.0 16 3976.0 17 4212.0 18 4886.0 19 6062.0 20 7685.0 21 9880.0 22 12233.0 23 14705.0 24 17009.0 25 19177.0 26 21784.0 27 24483.0 28 28328.0 29 34059.0 30 42768.0 31 53742.0 32 68313.0 33 90098.0 34 122183.0 35 174418.0 36 269525.0 37 362844.0 38 862188.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.729998478685715 19.305143881223806 12.820458166364912 25.144399473725564 2 15.513789637219086 21.278359050197082 41.1747692734962 22.033082039087628 3 18.648554667843595 25.18556690690058 29.998520480066603 26.167357945189217 4 11.782704495567291 17.11123315060844 39.5780776940219 31.52798465980236 5 12.377828925178745 38.55613589791064 36.564326819353774 12.501708357556835 6 30.65103209959513 38.2089376326721 18.61239873662935 12.527631531103417 7 27.080731635145366 31.747697549346586 23.07549563917769 18.096075176330356 8 23.808861070570593 38.81211267087619 21.093816609742763 16.28520964881045 9 26.2150870910632 15.126670311133847 20.842799753246165 37.81544284455679 10 14.405174474686836 29.54108133681731 35.70319827071704 20.350545917778813 11 33.755623429577355 22.143607052879883 25.92683147014128 18.173938047401478 12 23.154695929397644 24.594260465722783 33.20393803068374 19.047105574195836 13 29.071486844788907 22.532290367556833 26.130330042162207 22.265892745492057 14 19.868322725927708 23.21621721024396 28.397420787171473 28.518039276656854 15 21.926318235542915 33.07751012677061 23.620117793191532 21.376053844494933 16 20.77413163882327 28.57157783695857 29.809442847900335 20.84484767631782 17 20.646077805682648 29.4767774516405 29.006546289829743 20.870598452847112 18 20.361161682272677 27.777610600384172 31.944507135967047 19.916720581376104 19 22.958278269141232 26.943797326993952 28.25442958512318 21.843494818741636 20 22.9078192922602 28.079137060483756 29.181425351460444 19.831618295795597 21 21.982218893512304 27.328002567861937 27.8583763957316 22.83140214289416 22 22.336878630788206 29.32195193514559 28.207162289414807 20.1340071446514 23 21.217554484225747 28.78158873055777 28.89472617353453 21.10613061168195 24 22.57185058622321 27.13917555934873 29.85471059983181 20.434263254596246 25 21.853908000483145 28.18836742464296 29.263789039986328 20.69393553488757 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 229.0 1 229.0 2 425.0 3 621.0 4 621.0 5 621.0 6 1712.0 7 2803.0 8 2803.0 9 2803.0 10 4099.0 11 5395.0 12 5395.0 13 5395.0 14 8895.0 15 12395.0 16 12395.0 17 12395.0 18 22181.0 19 31967.0 20 31967.0 21 31967.0 22 51421.5 23 70876.0 24 70876.0 25 70876.0 26 102686.5 27 134497.0 28 134497.0 29 134497.0 30 176345.5 31 218194.0 32 218194.0 33 218194.0 34 239884.0 35 261574.0 36 261574.0 37 261574.0 38 273520.0 39 285466.0 40 285466.0 41 285466.0 42 294973.0 43 304480.0 44 304480.0 45 304480.0 46 305606.0 47 306732.0 48 306732.0 49 306732.0 50 288390.0 51 270048.0 52 270048.0 53 270048.0 54 233556.0 55 197064.0 56 197064.0 57 197064.0 58 165858.0 59 134652.0 60 134652.0 61 134652.0 62 108322.5 63 81993.0 64 81993.0 65 81993.0 66 62557.5 67 43122.0 68 43122.0 69 43122.0 70 31311.0 71 19500.0 72 19500.0 73 19500.0 74 13423.0 75 7346.0 76 7346.0 77 7346.0 78 4966.5 79 2587.0 80 2587.0 81 2587.0 82 1687.0 83 787.0 84 787.0 85 787.0 86 517.5 87 248.0 88 248.0 89 248.0 90 153.5 91 59.0 92 59.0 93 59.0 94 41.5 95 24.0 96 24.0 97 24.0 98 16.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.493490948180024 3 0.0 4 0.0 5 7.940926196196046E-4 6 8.776813164216682E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.6717739360412728E-4 14 0.0 15 0.0 16 0.0 17 6.269152260154773E-4 18 0.0 19 2.507660904061909E-4 20 3.761491356092863E-4 21 6.687095744165091E-4 22 0.0045555839757124685 23 1.2538304520309546E-4 24 0.0026748382976660365 25 0.0010448587100257956 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2392668.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.5888649042718 #Duplication Level Percentage of deduplicated Percentage of total 1 84.91912296165167 60.792636214909265 2 10.670525297686261 15.277815879873527 3 2.407871383910795 5.171303376289556 4 0.8138778354604677 2.3305836164544225 5 0.36986704035234325 1.3239180792163372 6 0.20344915401701688 0.8738816401087565 7 0.12409214644193468 0.6218531135118942 8 0.08097475586422413 0.46375126865762656 9 0.05576147350294444 0.3592710534118879 >10 0.30699278436059163 3.9343421190302923 >50 0.023124673746589827 1.1346002347712516 >100 0.019904172963900936 2.8913262043251367 >500 0.0020702826859276308 1.0077801296297377 >1k 0.0020702826859232645 2.542973239403192 >5k 2.957546694176092E-4 1.273963830407224 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6663 0.278475743396075 No Hit CTGTAGGACGTGGAATATGGCAAGA 6470 0.27040943415467583 No Hit GTATCAACGCAGAGTACTTTTTTTT 6107 0.2552380856851013 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5761 0.2407772411383443 No Hit CTTTAGGACGTGAAATATGGCGAGG 5084 0.21248246727084574 No Hit GGTATCAACGCAGAGTACTTTTTTT 3828 0.1599887656791498 No Hit TATCAACGCAGAGTACTTTTTTTTT 3707 0.15493164952262495 No Hit GTCCTACAGTGTGCATTTCTCATTT 3466 0.1448592115579763 No Hit CTGAAGGACCTGGAATATGGCGAGA 2842 0.11877953815573243 No Hit CTGTAGGACCTGGAATATGGCGAGA 2530 0.10573970145461051 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.179434840103182E-5 2 0.0 0.0 0.0 0.0 4.179434840103182E-5 3 0.0 0.0 0.0 4.179434840103182E-5 4.179434840103182E-5 4 0.0 0.0 0.0 4.179434840103182E-5 4.179434840103182E-5 5 0.0 0.0 0.0 4.179434840103182E-5 4.179434840103182E-5 6 0.0 0.0 0.0 4.179434840103182E-5 4.179434840103182E-5 7 0.0 0.0 0.0 4.179434840103182E-5 4.179434840103182E-5 8 0.0 0.0 0.0 4.179434840103182E-5 4.179434840103182E-5 9 0.0 0.0 0.0 8.358869680206364E-5 4.179434840103182E-5 10 0.0 0.0 0.0 8.358869680206364E-5 4.179434840103182E-5 11 0.0 0.0 0.0 8.358869680206364E-5 4.179434840103182E-5 12 0.0 4.179434840103182E-5 0.0 8.358869680206364E-5 8.358869680206364E-5 13 0.0 4.179434840103182E-5 0.0 8.358869680206364E-5 1.2538304520309546E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGC 105 9.822543E-11 14.392092 10 GGTATCA 1150 0.0 13.992028 1 TCCAACG 285 0.0 13.587839 18 GTCAACG 45 0.008231498 13.325741 2 ACCGCAA 50 0.0015672661 13.222735 8 GCGTGCG 100 1.0939402E-8 13.222735 9 CCAACGA 210 0.0 12.593606 19 TAGGACC 1180 0.0 12.486882 4 GGCGAGG 1495 0.0 12.383012 19 CGGTAGG 130 1.1768861E-9 12.300684 1 GTACCGG 55 0.003205636 12.02092 6 CGTTAGG 85 3.1733543E-5 11.758007 1 GGCCTAC 170 3.6379788E-12 11.758007 1 CGACTGT 60 0.0040262286 11.660024 1 GCGACTA 65 8.4251736E-4 11.624867 16 CTACCGT 90 7.961506E-6 11.544139 4 CACCTTT 745 0.0 11.156518 14 GTATCAA 2590 0.0 11.036183 1 CGACTAA 70 0.0015656493 10.794519 17 AGGACCT 2365 0.0 10.78311 5 >>END_MODULE