FastQCFastQC Report
Thu 26 May 2016
SRR1511792_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511792_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392668
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74990.3134158186593376No Hit
GTCCTACAGTGGACATTTCTAAATT74900.3130396695237283No Hit
CTGTAGGACGTGGAATATGGCAAGA67980.2841179804302143No Hit
GTCCTAAAGTGTGTATTTCTCATTT62180.2598772583576158No Hit
CTTTAGGACGTGAAATATGGCGAGG59210.2474643368825094No Hit
GGTATCAACGCAGAGTACTTTTTTT48880.20429077498424353No Hit
TATCAACGCAGAGTACTTTTTTTTT47180.19718573575606813No Hit
GTCCTACAGTGTGCATTTCTCATTT37350.15610189127785384No Hit
CTGAAGGACCTGGAATATGGCGAGA32870.1373780231941916No Hit
CTGTAGGACCTGGAATATGGCGAGA28640.11969901382055514No Hit
GTCCTTCAGTGTGCATTTCTCATTT25820.10791300757146416No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25080.1048202257897878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12600.013.6987291
TCCAACG3200.013.65318618
CCAACGA1600.013.65318619
TGTCGAG1700.013.40875418
TATGTCG1500.013.29646116
GCCGTAT550.002983234412.1368941
ATGTCGA1850.011.80766717
CCGGTTT2650.011.46864412
CGGTTTC2900.011.46246513
CCACCTT8650.011.30912813
ATTTAGA9300.011.2792951
GGCGAGG15300.011.23604119
TAGGACC11550.011.0212444
GCACCGT951.3649549E-510.9970726
CCAACGT2250.010.97531519
TCGGTGA803.7815687E-410.68465513
GTATCAA29400.010.6713851
GCCGGTT2850.010.66382811
TGTAGAA10550.010.5351632
GTACTAG1002.3013808E-510.4897451
Produced by FastQC (version 0.11.5)