FastQCFastQC Report
Thu 26 May 2016
SRR1511791_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511791_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719094
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT70130.2579167914018419No Hit
CTGTAGGACGTGGAATATGGCAAGA70110.2578432374901346No Hit
GTCCTAAAGTGTGTATTTCTCATTT66750.24548618032329889No Hit
CTTTAGGACGTGAAATATGGCGAGG57820.21264435874596466No Hit
GTATCAACGCAGAGTACTTTTTTTT56280.20698070754449827No Hit
GTCCTACAGTGTGCATTTCTCATTT35210.12949166156079928No Hit
TATCAACGCAGAGTACTTTTTTTTT34980.1286457915761647No Hit
GGTATCAACGCAGAGTACTTTTTTT34600.12724826725372496No Hit
CTGAAGGACCTGGAATATGGCGAGA31800.11695071961469519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG1300.014.6061991
GGTATCA13200.013.7792131
GTCAACG450.00823511613.3249532
TCCAACG2600.012.71530618
CCAACGA1650.012.59420819
TAGGACC12350.012.0836554
GTCCTAT3600.011.6593331
TCCGGAC600.006136437411.0193243
GGCGTGT600.00613727711.0191228
CGTTAGG1001.3705583E-510.9930871
CACCTTT10450.010.8456914
GTATCAA27650.010.8069471
ACCTTTT10450.010.75530915
GGCGAGG17400.010.63993519
ATTAGAC1606.020855E-1010.6257773
TACACCG803.9799808E-410.6257775
GCGCCAC1252.4288056E-710.57835713
GTCCTAA21150.010.5371081
GTCCTAC36700.010.5111011
AATGGCG1106.479324E-610.30416316
Produced by FastQC (version 0.11.5)