##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511791_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2719094 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.36471008357931 31.0 30.0 34.0 25.0 34.0 2 26.632116065130518 31.0 26.0 34.0 10.0 34.0 3 28.36890964416824 31.0 28.0 34.0 16.0 34.0 4 32.72517978414869 35.0 33.0 37.0 26.0 37.0 5 33.0902925753946 35.0 35.0 37.0 27.0 37.0 6 33.288994054637314 37.0 35.0 37.0 27.0 37.0 7 33.26773219314963 37.0 35.0 37.0 27.0 37.0 8 33.28425791826248 37.0 35.0 37.0 27.0 37.0 9 34.82599020114788 39.0 35.0 39.0 27.0 39.0 10 34.69202940391175 39.0 35.0 39.0 27.0 39.0 11 34.691527030694786 39.0 35.0 39.0 27.0 39.0 12 34.577563703204085 39.0 35.0 39.0 27.0 39.0 13 34.57247634690084 39.0 35.0 39.0 27.0 39.0 14 35.65097271370537 40.0 36.0 41.0 27.0 41.0 15 35.597783305762874 40.0 36.0 41.0 27.0 41.0 16 35.547161297108524 40.0 36.0 41.0 27.0 41.0 17 35.50393072104164 40.0 36.0 41.0 27.0 41.0 18 35.468937447546864 40.0 36.0 41.0 26.0 41.0 19 35.48818577070156 40.0 36.0 41.0 26.0 41.0 20 35.4352843263234 40.0 36.0 41.0 26.0 41.0 21 35.32331504537908 40.0 36.0 41.0 25.0 41.0 22 35.22044989985635 40.0 36.0 41.0 25.0 41.0 23 35.110107631439 40.0 36.0 41.0 25.0 41.0 24 35.003467331397886 40.0 36.0 41.0 24.0 41.0 25 34.89641954268591 40.0 35.0 41.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9881057135069575 1101 2 3.58562965162281 1101 3 2.2506676897963978 1101 4 1.2064741223911781 1101 5 0.6955966582201398 1101 6 0.7518569229565131 1101 7 0.5658754808859854 1101 8 0.5329688011771907 1101 9 0.7014130485563683 1101 10 0.7003742695464084 1101 11 0.630331870407403 1101 12 0.6316782403885597 1101 13 0.5799047835957012 1101 14 0.5994784691328192 1101 15 0.7504106045067402 1101 16 0.6125583187485617 1101 17 0.6183030755779058 1101 18 0.8969025743471093 1101 19 0.8305939502261168 1101 20 0.8074172608150647 1101 21 0.9003391464476493 1101 22 0.8411338823081422 1101 23 0.7639161426086574 1101 24 0.8368948098467328 1101 25 0.9438624729912632 1102 1 1.3137704221240867 1102 2 3.983928216760809 1102 3 2.358578610621784 1102 4 1.45151645055509 1102 5 1.106976738439478 1102 6 1.0069360374679377 1102 7 0.8776052170177451 1102 8 0.8469320572550671 1102 9 0.9500436807476973 1102 10 0.9620342689453807 1102 11 0.8801857679748366 1102 12 0.9797831513040833 1102 13 1.0115861194297793 1102 14 1.0491273106545478 1102 15 0.9779109182778427 1102 16 0.9717141595755763 1102 17 1.0990520251076035 1102 18 1.0111279839730756 1102 19 1.2005933668770155 1102 20 1.1870541348417092 1102 21 1.1069083643532807 1102 22 1.3389076100326065 1102 23 1.1889250254244317 1102 24 0.9697414296922631 1102 25 1.0854365256234928 1103 1 1.1060652204552888 1103 2 3.6494111325013954 1103 3 2.2473255288446587 1103 4 1.5039958688369879 1103 5 1.0385875930403117 1103 6 0.8626936908991212 1103 7 0.9493577574813514 1103 8 0.816372969764636 1103 9 0.7917300494277342 1103 10 0.8898542425711042 1103 11 0.9807211793225221 1103 12 0.7694013987973491 1103 13 0.9242034461841584 1103 14 1.1333138732631056 1103 15 0.9092372881720436 1103 16 0.9049938112462286 1103 17 0.9411097247443649 1103 18 1.1211518962027114 1103 19 1.1188391738051564 1103 20 1.178130724802621 1103 21 1.1558415888617972 1103 22 1.2257860691271816 1103 23 1.2240078750606855 1103 24 1.503526138681444 1103 25 1.3534914394349116 1104 1 1.4938725790698513 1104 2 4.0248665399739 1104 3 2.4536588097796965 1104 4 1.7853418863455914 1104 5 1.4210492207586256 1104 6 1.2167420273795102 1104 7 0.997101758350027 1104 8 1.0677844093857018 1104 9 1.2252405584424508 1104 10 1.3543716006111808 1104 11 1.2856046057417458 1104 12 1.3806464517354087 1104 13 1.2631940593136548 1104 14 1.498599434502509 1104 15 1.4165569512320673 1104 16 1.5302476746735465 1104 17 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-1.3951939405020255 2315 18 -1.4551222321885433 2315 19 -1.4648865620465372 2315 20 -1.4405873601696513 2315 21 -1.5076942616658116 2315 22 -1.5702758128146144 2315 23 -1.6074652634677449 2315 24 -1.7039431171953794 2315 25 -1.8742487681182212 2316 1 -1.572501102662745 2316 2 -1.3242792367420684 2316 3 -0.9584200937669216 2316 4 -1.195551989478247 2316 5 -1.2400878091994123 2316 6 -1.3921876177252273 2316 7 -1.3429482320453303 2316 8 -1.4753624042383393 2316 9 -1.4478927323552142 2316 10 -1.621585917382923 2316 11 -1.7348480186267992 2316 12 -1.6168859475942483 2316 13 -1.5698911492955716 2316 14 -1.779462511907873 2316 15 -2.3704255155845004 2316 16 -2.0117946919147514 2316 17 -1.828050717424098 2316 18 -1.787722215064207 2316 19 -1.7919846401058166 2316 20 -2.003041018756498 2316 21 -1.8993169953997295 2316 22 -1.9527540778702672 2316 23 -1.9999236969743208 2316 24 -1.7969711837046134 2316 25 -1.9155181613445862 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 109148.0 3 4.0 4 1055.0 5 4926.0 6 5907.0 7 5282.0 8 4773.0 9 4502.0 10 4384.0 11 4237.0 12 4282.0 13 4300.0 14 4112.0 15 4382.0 16 4597.0 17 5015.0 18 5619.0 19 6732.0 20 8863.0 21 11462.0 22 14498.0 23 16994.0 24 19707.0 25 22604.0 26 25071.0 27 28300.0 28 32615.0 29 39007.0 30 48187.0 31 61927.0 32 77557.0 33 101919.0 34 137795.0 35 195000.0 36 304131.0 37 406283.0 38 983917.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.666086571483 19.27682529548445 12.695589045468822 25.361499087563725 2 15.589531307395854 21.36172540889196 40.95052204934721 22.09822123436498 3 18.412971379437415 25.290740224501253 29.94262059347709 26.35366780258424 4 11.727766675223439 17.29171554937049 39.58943677563188 31.39108099977419 5 12.408599667093528 38.44157697340499 36.628943150666295 12.52088020883519 6 30.53672763113859 38.21166796000103 18.81154218170183 12.44006222715855 7 27.232673824442994 31.73178271880266 23.22858275587383 17.806960700880513 8 23.93856188862908 38.47461691283935 21.171022406728124 16.415798791803446 9 26.2957440971147 15.076418836568356 20.90887626540311 37.718960800913834 10 14.36566738774018 29.434179178799997 35.78956814291819 20.41058529054163 11 33.63690258593488 22.095926069492265 26.049191385071644 18.217979959501214 12 23.199786399440402 24.663582796328484 33.081423444720926 19.05520735951019 13 29.049829170788755 22.486714305153562 26.07585626073429 22.38760026332339 14 19.91001414441722 23.154403635916964 28.465327053790713 28.470255165875102 15 22.002034501197826 32.88543904697668 23.479401594795913 21.633124857029586 16 20.911046105798476 28.505744928273902 29.567900190283968 21.01530877564365 17 20.798255585806814 29.48112210053478 28.856719499507 20.86390281415141 18 20.56159147127683 27.63383685889491 31.660913524872626 20.143658144955637 19 23.041894286380476 27.103448884586783 28.09532776135475 21.75932906767799 20 23.029689854314533 27.959267060109 29.042833284971337 19.968209800605134 21 22.127782426008846 27.133749528791963 27.756130301665088 22.982337743534107 22 22.505730064671027 29.319394834556334 27.897397270753277 20.27747783001936 23 21.181713266884532 28.767056282883292 28.82446527987714 21.226765170355037 24 22.61277221434159 27.201666174459177 29.65002760162307 20.535534009576164 25 21.960286450671408 28.128935166115497 29.006293323604528 20.90448505960857 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 206.0 1 206.0 2 422.0 3 638.0 4 638.0 5 638.0 6 1706.0 7 2774.0 8 2774.0 9 2774.0 10 4080.5 11 5387.0 12 5387.0 13 5387.0 14 9201.5 15 13016.0 16 13016.0 17 13016.0 18 24229.0 19 35442.0 20 35442.0 21 35442.0 22 57348.0 23 79254.0 24 79254.0 25 79254.0 26 114397.5 27 149541.0 28 149541.0 29 149541.0 30 194234.5 31 238928.0 32 238928.0 33 238928.0 34 266967.0 35 295006.0 36 295006.0 37 295006.0 38 311878.0 39 328750.0 40 328750.0 41 328750.0 42 339476.0 43 350202.0 44 350202.0 45 350202.0 46 352188.5 47 354175.0 48 354175.0 49 354175.0 50 333778.0 51 313381.0 52 313381.0 53 313381.0 54 269438.5 55 225496.0 56 225496.0 57 225496.0 58 189198.0 59 152900.0 60 152900.0 61 152900.0 62 122673.5 63 92447.0 64 92447.0 65 92447.0 66 70394.0 67 48341.0 68 48341.0 69 48341.0 70 34873.0 71 21405.0 72 21405.0 73 21405.0 74 14668.5 75 7932.0 76 7932.0 77 7932.0 78 5289.5 79 2647.0 80 2647.0 81 2647.0 82 1750.0 83 853.0 84 853.0 85 853.0 86 555.5 87 258.0 88 258.0 89 258.0 90 169.5 91 81.0 92 81.0 93 81.0 94 54.5 95 28.0 96 28.0 97 28.0 98 17.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.487930906397499 3 0.0 4 0.0 5 4.4132347024413283E-4 6 8.826469404882657E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.4710782341471093E-4 14 0.0 15 0.0 16 0.0 17 5.51654337805166E-4 18 0.0 19 4.4132347024413283E-4 20 6.252082495125215E-4 21 6.987621612198769E-4 22 0.0037512494970751284 23 2.9421564682942185E-4 24 0.0018756247485375642 25 8.090930287809102E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2719094.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.80461055327024 #Duplication Level Percentage of deduplicated Percentage of total 1 85.3853740818161 62.16448906971917 2 10.450606341118563 15.21704649421347 3 2.3097010878921824 5.0447066459536485 4 0.7447150581610111 2.1687475913027368 5 0.34108698510263946 1.2416352557593378 6 0.17838166115198972 0.7792204422009618 7 0.11389000933997064 0.5804202443133412 8 0.07749666905377087 0.45136918477083665 9 0.050951674533803434 0.3338565139323483 >10 0.305503453896063 3.9587564730609697 >50 0.02082165807221466 1.0272557227192387 >100 0.017535375978580906 2.579210523778299 >500 0.001737949247155546 0.8472348024254203 >1k 0.0019424138643709735 2.4111172631189777 >5k 2.555807716277597E-4 1.1949337727312854 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 7013 0.2579167914018419 No Hit CTGTAGGACGTGGAATATGGCAAGA 7011 0.2578432374901346 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6675 0.24548618032329889 No Hit CTTTAGGACGTGAAATATGGCGAGG 5782 0.21264435874596466 No Hit GTATCAACGCAGAGTACTTTTTTTT 5628 0.20698070754449827 No Hit GTCCTACAGTGTGCATTTCTCATTT 3521 0.12949166156079928 No Hit TATCAACGCAGAGTACTTTTTTTTT 3498 0.1286457915761647 No Hit GGTATCAACGCAGAGTACTTTTTTT 3460 0.12724826725372496 No Hit CTGAAGGACCTGGAATATGGCGAGA 3180 0.11695071961469519 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.355391170735546E-5 0.0 10 0.0 0.0 0.0 7.355391170735546E-5 3.677695585367773E-5 11 0.0 0.0 0.0 1.4710782341471093E-4 3.677695585367773E-5 12 0.0 0.0 0.0 1.4710782341471093E-4 3.677695585367773E-5 13 0.0 0.0 0.0 1.4710782341471093E-4 3.677695585367773E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 130 0.0 14.606199 1 GGTATCA 1320 0.0 13.779213 1 GTCAACG 45 0.008235116 13.324953 2 TCCAACG 260 0.0 12.715306 18 CCAACGA 165 0.0 12.594208 19 TAGGACC 1235 0.0 12.083655 4 GTCCTAT 360 0.0 11.659333 1 TCCGGAC 60 0.0061364374 11.019324 3 GGCGTGT 60 0.006137277 11.019122 8 CGTTAGG 100 1.3705583E-5 10.993087 1 CACCTTT 1045 0.0 10.84569 14 GTATCAA 2765 0.0 10.806947 1 ACCTTTT 1045 0.0 10.755309 15 GGCGAGG 1740 0.0 10.639935 19 ATTAGAC 160 6.020855E-10 10.625777 3 TACACCG 80 3.9799808E-4 10.625777 5 GCGCCAC 125 2.4288056E-7 10.578357 13 GTCCTAA 2115 0.0 10.537108 1 GTCCTAC 3670 0.0 10.511101 1 AATGGCG 110 6.479324E-6 10.304163 16 >>END_MODULE