FastQCFastQC Report
Thu 26 May 2016
SRR1511791_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511791_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719094
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT79940.2939949850942998No Hit
CTGTAGGACGTGGAATATGGCAAGA78300.28796356433429665No Hit
GTCCTAAAGTGTGTATTTCTCATTT74690.27468708327111896No Hit
GTATCAACGCAGAGTACTTTTTTTT67010.2464423811754945No Hit
CTTTAGGACGTGAAATATGGCGAGG64750.23813078915256333No Hit
GGTATCAACGCAGAGTACTTTTTTT44670.16428266179837844No Hit
TATCAACGCAGAGTACTTTTTTTTT44320.1629954683434997No Hit
GTCCTACAGTGTGCATTTCTCATTT38510.14162805699251294No Hit
CTGAAGGACCTGGAATATGGCGAGA36520.1343094427776311No Hit
TTTCTAAATTTTCCACCTTTTTCAG29430.10823458107737356No Hit
ATTTAGAAATGTCCACTGTAGGACG28940.10643251024054336No Hit
GTCCTTCAGTGTGCATTTCTCATTT28540.10496143200639625No Hit
CTGTAGGACCTGGAATATGGCGAGA27460.10098952077419905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA456.779891E-414.7733886
GGTATCA14050.013.3057411
TAGGACC13100.012.2554654
CGTGCGC1001.938055E-611.39661310
ACCGTGC752.0806397E-411.3966138
GGCGAGG16700.011.14720819
TACACCG600.005878509511.0830975
ACCTTTT10650.010.96877315
TCCAACG2350.010.91245318
CACCTTT10900.010.54293214
AGGACCT26050.010.3567855
AGCACCG1201.5252226E-610.2914485
GGCGTGC1201.5306305E-610.2886098
CCACCTT11100.010.26740713
TGTCGAG2151.8189894E-1210.16051918
TAATACT3850.010.1166684
GGACCTG26750.010.0474816
CCTTTTT11550.010.03183616
TGTAGGA42000.09.9974062
GTCCTAC38900.09.9793921
Produced by FastQC (version 0.11.5)