FastQCFastQC Report
Thu 26 May 2016
SRR1511790_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511790_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2759387
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT77060.27926492369500905No Hit
CTGTAGGACGTGGAATATGGCAAGA70490.25545528771426407No Hit
GTCCTAAAGTGTGTATTTCTCATTT68650.24878714004233551No Hit
GTATCAACGCAGAGTACTTTTTTTT67240.24367730948939023No Hit
CTTTAGGACGTGAAATATGGCGAGG61850.2241439856025994No Hit
GGTATCAACGCAGAGTACTTTTTTT42690.1547082739753431No Hit
TATCAACGCAGAGTACTTTTTTTTT42460.153874755516352No Hit
GTCCTACAGTGTGCATTTCTCATTT35630.1291228812776171No Hit
CTGAAGGACCTGGAATATGGCGAGA33280.12060649702270831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13950.013.3915431
CGTTAGG1059.8734745E-913.3199311
TAGGACC12600.013.0439254
TCAAGCG658.4229285E-411.6253563
GGCGAGG17350.011.324038519
TCCAACG2100.011.24496718
GTGGTAT5300.011.1208851
GTATCAA31450.011.0858251
AAATGTC10850.011.0557417
ACCTTTT11100.010.97712115
TGGCGAA1606.020855E-1010.62649318
AATGTCC11400.010.6052038
CGATATT854.1504423E-410.5775931
GTCCTAA22000.010.5348541
GGCCTAC2009.094947E-1210.4894451
AGGACCT25600.010.4049215
TTAGGAC24900.010.3560213
CCACCTT11700.010.33346213
CACCTTT12350.010.17200214
GTCCTAC38450.010.1328471
Produced by FastQC (version 0.11.5)