##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511790_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2759387 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.2781110442283 31.0 30.0 34.0 24.0 34.0 2 26.569959922258096 31.0 26.0 34.0 10.0 34.0 3 28.29302522625496 31.0 28.0 34.0 16.0 34.0 4 32.639251036552686 35.0 32.0 37.0 26.0 37.0 5 32.99925563177619 35.0 35.0 37.0 27.0 37.0 6 33.199344999450965 37.0 35.0 37.0 27.0 37.0 7 33.178990116283074 37.0 35.0 37.0 26.0 37.0 8 33.192053524931445 37.0 35.0 37.0 26.0 37.0 9 34.72576626620333 39.0 35.0 39.0 27.0 39.0 10 34.58909569407988 39.0 35.0 39.0 27.0 39.0 11 34.58363687297215 39.0 35.0 39.0 27.0 39.0 12 34.46380880971027 39.0 35.0 39.0 27.0 39.0 13 34.456141164686215 39.0 35.0 39.0 27.0 39.0 14 35.52679091406896 40.0 36.0 41.0 26.0 41.0 15 35.473704848214474 40.0 36.0 41.0 26.0 41.0 16 35.42330379899594 40.0 36.0 41.0 26.0 41.0 17 35.376540151852566 40.0 36.0 41.0 25.0 41.0 18 35.343106276865115 40.0 36.0 41.0 25.0 41.0 19 35.362361278066466 40.0 36.0 41.0 25.0 41.0 20 35.30081282545725 40.0 36.0 41.0 25.0 41.0 21 35.1868929584723 40.0 36.0 41.0 25.0 41.0 22 35.08974710687555 40.0 36.0 41.0 24.0 41.0 23 34.970496345746355 40.0 35.0 41.0 24.0 41.0 24 34.85991526378866 40.0 35.0 41.0 22.0 41.0 25 34.748584377617206 39.0 35.0 41.0 21.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0924330337813473 1101 2 3.5787351359099837 1101 3 2.1545448893348507 1101 4 1.2814071038131658 1101 5 0.6241277671027419 1101 6 0.8212018494085385 1101 7 0.6621263356424052 1101 8 0.6028742591616165 1101 9 0.8170280073563774 1101 10 0.7885367018246185 1101 11 0.5764004431007237 1101 12 0.7239930531348691 1101 13 0.5492532494973759 1101 14 0.6234397620703689 1101 15 0.8204698878994421 1101 16 0.7445250301033042 1101 17 0.7632664152419508 1101 18 0.9426755687426365 1101 19 0.9105580983660033 1101 20 0.7578113823800408 1101 21 0.9636060977126064 1101 22 0.7892931483605992 1101 23 0.6781576248446299 1101 24 0.715535419879636 1101 25 0.8007178627937321 1102 1 1.5783783484194167 1102 2 4.200727388698937 1102 3 2.4723659718859423 1102 4 1.650087017250108 1102 5 1.2635549567157156 1102 6 1.1822199270217055 1102 7 1.0921664012564705 1102 8 1.0757033410000076 1102 9 1.1539890003275985 1102 10 1.13331307104243 1102 11 1.2466356029126828 1102 12 1.1817046461791847 1102 13 1.3142815667382095 1102 14 1.4557165910658156 1102 15 1.2413655246180824 1102 16 1.3372140088367743 1102 17 1.3416950319372276 1102 18 1.3463427889422093 1102 19 1.3413321209462339 1102 20 1.3791199584731544 1102 21 1.1994900805444288 1102 22 1.3909957935415065 1102 23 1.5422481981713148 1102 24 1.3074593961109002 1102 25 1.3389706875156975 1103 1 1.2357535949591352 1103 2 3.783817818594386 1103 3 2.3694825480819866 1103 4 1.4703442912626414 1103 5 1.1409832638387485 1103 6 0.921891440121037 1103 7 1.017305598016982 1103 8 0.7335411222298305 1103 9 0.7754872636407413 1103 10 1.0135689487238224 1103 11 1.0915119403360976 1103 12 0.9347547570129677 1103 13 1.1006865376770634 1103 14 1.3226113251520104 1103 15 1.0121664950682216 1103 16 1.0755764614924175 1103 17 1.1340605914296589 1103 18 1.0592920148511382 1103 19 1.041923816266781 1103 20 1.0357272228859102 1103 21 1.2376273748037576 1103 22 1.1640745291636563 1103 23 1.3313760148330402 1103 24 1.2334363915553723 1103 25 1.2665447367014622 1104 1 1.4009680634255268 1104 2 4.005391505666211 1104 3 2.3679128645236283 1104 4 1.6589383720320399 1104 5 1.395213869652224 1104 6 1.1351297871543977 1104 7 0.9912927614589364 1104 8 1.0212976373541878 1104 9 1.118507422315517 1104 10 1.1424788007579068 1104 11 1.0829120433773483 1104 12 1.1345096082258763 1104 13 1.2273485153470887 1104 14 1.4643753931967325 1104 15 1.3193750538986961 1104 16 1.2844600109743283 1104 17 1.1473423489251928 1104 18 1.2788494125298797 1104 19 1.2691932122503573 1104 20 1.1413704423295457 1104 21 1.22567308986455 1104 22 1.305071647790328 1104 23 1.3506310081691097 1104 24 1.36427424316566 1104 25 1.1430930705319753 1105 1 1.112568890543514 1105 2 3.7881750501520948 1105 3 2.0908301262186733 1105 4 1.2652861124232544 1105 5 0.9497916841749117 1105 6 0.7168002426440907 1105 7 0.623657401003527 1105 8 0.6246026545341863 1105 9 0.7587426678702585 1105 10 0.6279375233353477 1105 11 0.7047804568041087 1105 12 0.553037652713698 1105 13 0.6863963170603498 1105 14 0.7087844097715603 1105 15 0.8588495963138101 1105 16 0.7636833325326791 1105 17 0.6539233751864586 1105 18 0.8169101824119664 1105 19 0.6674470080970778 1105 20 0.7223597733652625 1105 21 0.8495817494967994 1105 22 0.6989030487891696 1105 23 0.6709691699312543 1105 24 0.7144560500843014 1105 25 0.6940204511095942 1106 1 0.9532310592852546 1106 2 3.6440006501495077 1106 3 2.1702528475872676 1106 4 1.1205162846460084 1106 5 0.8566285084954117 1106 6 0.7403986580718325 1106 7 0.6772947513580192 1106 8 0.6772335523991586 1106 9 0.6738253823480065 1106 10 0.7375384524679589 1106 11 0.5867780272129153 1106 12 0.5763363322907367 1106 13 0.5137850241522699 1106 14 0.5376046669329497 1106 15 0.634394677200433 1106 16 0.6879017628097159 1106 17 0.580977568657616 1106 18 0.6234194486657216 1106 19 0.649731373790992 1106 20 0.6427089745279702 1106 21 0.712466266271818 1106 22 0.5129555324880997 1106 23 0.578122229323597 1106 24 0.5722371313009091 1106 25 0.6881122741279526 1107 1 1.1771452756327037 1107 2 3.6073772914503763 1107 3 2.0728718713073206 1107 4 1.3233227287712026 1107 5 1.090306465346373 1107 6 0.9047621187778532 1107 7 0.7094443300934117 1107 8 0.7989682027463942 1107 9 0.8778794660739493 1107 10 0.8756853273307215 1107 11 0.895965391156679 1107 12 0.8158078537993845 1107 13 0.8580093389307208 1107 14 1.0339469107080816 1107 15 0.9847367390444575 1107 16 1.0083851663015437 1107 17 0.9651197634995583 1107 18 0.8836082178704316 1107 19 0.9402408436186604 1107 20 0.9832157781239346 1107 21 0.9111243675384344 1107 22 1.149510823860176 1107 23 1.156114211808024 1107 24 0.9929476349991262 1107 25 1.0271910753901778 1108 1 0.9314441692301685 1108 2 3.5876644092393164 1108 3 1.9902008917383682 1108 4 1.1963947167528133 1108 5 0.7683990780161167 1108 6 0.6971954063967587 1108 7 0.6038202058579927 1108 8 0.5805474623004088 1108 9 0.561888044710031 1108 10 0.6072220554476502 1108 11 0.5905339484286287 1108 12 0.45638608983263396 1108 13 0.6313500277112496 1108 14 0.7458361895940797 1108 15 0.689567634688963 1108 16 0.6127477107613899 1108 17 0.6042135220444109 1108 18 0.6109317004370638 1108 19 0.6413520339350001 1108 20 0.5857379535204927 1108 21 0.5495039490439879 1108 22 0.4893636049746206 1108 23 0.6647226385119112 1108 24 0.5305031067389976 1108 25 0.5940497497286401 1109 1 0.4920862308178684 1109 2 2.478549492113082 1109 3 1.224409194761968 1109 4 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2314 8 -0.5411427869413359 2314 9 -0.5836155337772766 2314 10 -0.850491044340238 2314 11 -0.883830712234726 2314 12 -0.7703533068879338 2314 13 -0.8569379996881352 2314 14 -1.6587071399489162 2314 15 -1.3496710334345678 2314 16 -1.183975732544063 2314 17 -1.1878247835837143 2314 18 -0.9647348037865129 2314 19 -1.1692579513143784 2314 20 -0.9810319859877055 2314 21 -0.867829023014167 2314 22 -0.8570192144513058 2314 23 -0.8067829801560364 2314 24 -0.8580565198445385 2314 25 -0.7460142404293393 2315 1 -4.73339804462633 2315 2 -13.744658833228216 2315 3 -7.31228576301077 2315 4 -3.7349598812315215 2315 5 -2.383865309021161 2315 6 -1.8327689606830049 2315 7 -1.6415577283129892 2315 8 -1.6140292012565354 2315 9 -1.6431038120266521 2315 10 -1.771933711867419 2315 11 -1.7367350647343613 2315 12 -1.6521648281060948 2315 13 -1.6437652408113053 2315 14 -1.7774162353291345 2315 15 -1.7881289906110922 2315 16 -1.8253476758908391 2315 17 -1.8197705786132445 2315 18 -1.6433906821907556 2315 19 -1.7778874566398812 2315 20 -1.7842439821530647 2315 21 -1.8215648590737175 2315 22 -1.8452326357480189 2315 23 -1.7543787093253016 2315 24 -1.8703084845157676 2315 25 -1.862960935446516 2316 1 -1.5969964177627212 2316 2 -1.3008871191816063 2316 3 -1.0502538550820795 2316 4 -1.1200006689038169 2316 5 -1.335130746598825 2316 6 -1.4519332018294229 2316 7 -1.4848175382312583 2316 8 -1.6701205103516905 2316 9 -1.6507627292915572 2316 10 -1.9904907365657536 2316 11 -1.9610717270637252 2316 12 -1.763477187847684 2316 13 -1.7769179087526155 2316 14 -1.996006120186422 2316 15 -2.4209759162039077 2316 16 -2.2752527207467423 2316 17 -2.3589423883114904 2316 18 -2.1921992692646413 2316 19 -2.2362494384360687 2316 20 -2.4177192689918954 2316 21 -2.425316971321166 2316 22 -2.304304593424135 2316 23 -2.3236638187085603 2316 24 -2.234066336575758 2316 25 -2.251856719809581 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 115607.0 3 2.0 4 1032.0 5 5251.0 6 6204.0 7 5608.0 8 4964.0 9 4870.0 10 4717.0 11 4570.0 12 4342.0 13 4361.0 14 4447.0 15 4577.0 16 4809.0 17 5249.0 18 5974.0 19 7108.0 20 9313.0 21 11941.0 22 14674.0 23 17508.0 24 20141.0 25 22953.0 26 26155.0 27 29110.0 28 33135.0 29 40238.0 30 49464.0 31 62433.0 32 79848.0 33 103875.0 34 139898.0 35 199086.0 36 308139.0 37 414978.0 38 982806.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.74971216433215 19.214339996528214 12.847527367491404 25.188420471648232 2 15.729377994685315 21.176528757986308 40.85635207706256 22.237741170265814 3 18.43536263670156 25.30424329751499 29.829922370439522 26.430471695343932 4 11.970484748967797 17.111590364091736 39.11223760929511 31.80568727764536 5 12.642410172463217 38.32424128542338 36.239961933353655 12.79338660875975 6 30.82093435888218 37.80963611957521 18.589401631098134 12.78002789044448 7 27.29983144807162 31.727698941830194 23.06726095324795 17.905208656850235 8 24.277167356372992 38.16492576068525 20.936860251932764 16.62104663100899 9 26.385751618022407 15.08215411611347 20.78258685715342 37.749507408710706 10 14.879935289975634 29.02644681590513 35.28838107884106 20.805236815278175 11 33.689257795300186 22.041308450028936 25.92782382463931 18.34160993003156 12 23.602742203250216 24.510480045024494 32.59890693114087 19.287870820584427 13 29.048000258028882 22.518746052103715 25.95013156936284 22.48312212050456 14 20.21282263053352 23.016561286981492 28.144801725890567 28.62581435659442 15 22.1473464939858 32.68729612772692 23.433610435941027 21.731746942346252 16 21.191916900384033 28.163791450782366 29.297811434206224 21.346480214627377 17 21.023298054774784 29.327651364150974 28.55243874330826 21.096611837765984 18 20.793350117254302 27.325271881037345 31.48978378168775 20.391594220020608 19 23.39802665673206 26.792306092170048 27.990619625147406 21.819047625950486 20 23.40906908267271 27.573889014722536 28.87338618346546 20.143655719139296 21 22.38957529783077 26.8969826072555 27.592576418739213 23.120865676174517 22 22.91148943110056 28.98601987841009 27.741924962173037 20.360565728316317 23 21.335538174852196 28.600027107519004 28.711610063412753 21.35282465421605 24 22.935880226257332 26.867224578666754 29.428964069616747 20.767931125459167 25 22.192819279536096 27.82752963457844 28.896400289777237 21.08325079610823 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 313.0 1 313.0 2 508.0 3 703.0 4 703.0 5 703.0 6 1941.5 7 3180.0 8 3180.0 9 3180.0 10 4608.0 11 6036.0 12 6036.0 13 6036.0 14 9697.0 15 13358.0 16 13358.0 17 13358.0 18 24069.5 19 34781.0 20 34781.0 21 34781.0 22 56571.0 23 78361.0 24 78361.0 25 78361.0 26 114005.5 27 149650.0 28 149650.0 29 149650.0 30 193142.5 31 236635.0 32 236635.0 33 236635.0 34 263759.0 35 290883.0 36 290883.0 37 290883.0 38 305844.5 39 320806.0 40 320806.0 41 320806.0 42 332486.0 43 344166.0 44 344166.0 45 344166.0 46 350068.5 47 355971.0 48 355971.0 49 355971.0 50 337567.5 51 319164.0 52 319164.0 53 319164.0 54 278868.0 55 238572.0 56 238572.0 57 238572.0 58 202490.5 59 166409.0 60 166409.0 61 166409.0 62 135561.0 63 104713.0 64 104713.0 65 104713.0 66 80150.5 67 55588.0 68 55588.0 69 55588.0 70 40446.5 71 25305.0 72 25305.0 73 25305.0 74 17566.0 75 9827.0 76 9827.0 77 9827.0 78 6679.5 79 3532.0 80 3532.0 81 3532.0 82 2286.0 83 1040.0 84 1040.0 85 1040.0 86 661.5 87 283.0 88 283.0 89 283.0 90 177.5 91 72.0 92 72.0 93 72.0 94 52.0 95 32.0 96 32.0 97 32.0 98 19.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.450123523811629 3 0.0 4 0.0 5 5.798389279937899E-4 6 6.885587269926255E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.1743959799767123E-4 14 0.0 15 0.0 16 0.0 17 5.43598994994178E-4 18 0.0 19 3.261593969965068E-4 20 4.3487919599534245E-4 21 6.523187939930136E-4 22 0.004058872495956529 23 1.8119966499805936E-4 24 0.0018482365829802052 25 9.422382579899087E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2759387.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.86465271091205 #Duplication Level Percentage of deduplicated Percentage of total 1 81.48760461145774 55.301279872006944 2 12.482649167109638 16.942613012761022 3 3.285418577291715 6.688913722736429 4 1.172722121087668 3.1834551789607493 5 0.5241803389817395 1.7786658331441954 6 0.28012032396541287 1.1406161101908552 7 0.161178184543437 0.765681106303095 8 0.10855991367135034 0.5893904671706227 9 0.07450302757367855 0.45505098829792795 >10 0.3738206372881221 4.457364723688886 >50 0.024733339799757302 1.1607734171643735 >100 0.019912534478848637 2.7298410009419296 >500 0.002379738835825 1.0974302213386933 >1k 0.0019470590473598722 2.441551921112988 >5k 2.704248676888711E-4 1.2673724241812168 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 7706 0.27926492369500905 No Hit CTGTAGGACGTGGAATATGGCAAGA 7049 0.25545528771426407 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6865 0.24878714004233551 No Hit GTATCAACGCAGAGTACTTTTTTTT 6724 0.24367730948939023 No Hit CTTTAGGACGTGAAATATGGCGAGG 6185 0.2241439856025994 No Hit GGTATCAACGCAGAGTACTTTTTTT 4269 0.1547082739753431 No Hit TATCAACGCAGAGTACTTTTTTTTT 4246 0.153874755516352 No Hit GTCCTACAGTGTGCATTTCTCATTT 3563 0.1291228812776171 No Hit CTGAAGGACCTGGAATATGGCGAGA 3328 0.12060649702270831 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.623993299961187E-5 3.623993299961187E-5 5 0.0 0.0 0.0 3.623993299961187E-5 3.623993299961187E-5 6 0.0 0.0 0.0 3.623993299961187E-5 3.623993299961187E-5 7 0.0 0.0 0.0 3.623993299961187E-5 3.623993299961187E-5 8 0.0 3.623993299961187E-5 0.0 3.623993299961187E-5 7.247986599922374E-5 9 0.0 3.623993299961187E-5 0.0 3.623993299961187E-5 7.247986599922374E-5 10 3.623993299961187E-5 3.623993299961187E-5 0.0 3.623993299961187E-5 7.247986599922374E-5 11 3.623993299961187E-5 3.623993299961187E-5 0.0 7.247986599922374E-5 7.247986599922374E-5 12 3.623993299961187E-5 3.623993299961187E-5 0.0 7.247986599922374E-5 7.247986599922374E-5 13 3.623993299961187E-5 3.623993299961187E-5 0.0 7.247986599922374E-5 7.247986599922374E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1395 0.0 13.391543 1 CGTTAGG 105 9.8734745E-9 13.319931 1 TAGGACC 1260 0.0 13.043925 4 TCAAGCG 65 8.4229285E-4 11.625356 3 GGCGAGG 1735 0.0 11.3240385 19 TCCAACG 210 0.0 11.244967 18 GTGGTAT 530 0.0 11.120885 1 GTATCAA 3145 0.0 11.085825 1 AAATGTC 1085 0.0 11.055741 7 ACCTTTT 1110 0.0 10.977121 15 TGGCGAA 160 6.020855E-10 10.626493 18 AATGTCC 1140 0.0 10.605203 8 CGATATT 85 4.1504423E-4 10.577593 1 GTCCTAA 2200 0.0 10.534854 1 GGCCTAC 200 9.094947E-12 10.489445 1 AGGACCT 2560 0.0 10.404921 5 TTAGGAC 2490 0.0 10.356021 3 CCACCTT 1170 0.0 10.333462 13 CACCTTT 1235 0.0 10.172002 14 GTCCTAC 3845 0.0 10.132847 1 >>END_MODULE