FastQCFastQC Report
Thu 26 May 2016
SRR1511790_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511790_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2759387
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT84820.3073871117027079No Hit
GTATCAACGCAGAGTACTTTTTTTT81590.29568161334383325No Hit
CTGTAGGACGTGGAATATGGCAAGA78620.28491835324294856No Hit
GTCCTAAAGTGTGTATTTCTCATTT74420.2696975813831115No Hit
CTTTAGGACGTGAAATATGGCGAGG73060.26476895049516436No Hit
GGTATCAACGCAGAGTACTTTTTTT54360.19700027578589013No Hit
TATCAACGCAGAGTACTTTTTTTTT54190.19638419692489673No Hit
CTGAAGGACCTGGAATATGGCGAGA40300.14604692998843583No Hit
GTCCTACAGTGTGCATTTCTCATTT38960.14119077896648785No Hit
TTTCTAAATTTTCCACCTTTTTCAG31240.11321355069078749No Hit
CTGTAGGACCTGGAATATGGCGAGA29700.10763260100884726No Hit
ATTTAGAAATGTCCACTGTAGGACG29070.10534948522987171No Hit
GTCCTTCAGTGTGCATTTCTCATTT28820.10444348690488142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17750.013.106441
TAGGACC11800.012.8003964
TAGTACG604.0950326E-412.666224
GGCGAGG20650.011.31474119
GAACGTG1104.989197E-711.2244356
CACCTTT10800.010.90465114
AGGACCT25700.010.7933935
TTAGGAC26550.010.7340843
TAGGACT5250.010.6758144
TGTAGGA41800.010.6359882
ACCTTTT12550.010.51925415
AGTCGTC2450.010.4667428
GTGCGCC1002.406928E-510.44735911
CTGTAGG42000.010.3970341
CCACCTT11700.010.39053513
GGACCTG24800.010.3784256
GTCCTAA21600.010.3730731
GGACGTG44600.010.3706976
GACGTGA23950.010.270897
TGGCGAG44650.010.23182718
Produced by FastQC (version 0.11.5)