FastQCFastQC Report
Thu 26 May 2016
SRR1511789_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511789_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469683
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT73980.2995526146473049No Hit
CTGTAGGACGTGGAATATGGCAAGA66850.27068251269494914No Hit
GTCCTAAAGTGTGTATTTCTCATTT64730.2620984150597465No Hit
GTATCAACGCAGAGTACTTTTTTTT59900.24254124922105388No Hit
CTTTAGGACGTGAAATATGGCGAGG56310.22800497067842312No Hit
GGTATCAACGCAGAGTACTTTTTTT38200.15467572153997092No Hit
TATCAACGCAGAGTACTTTTTTTTT36230.1466989893034855No Hit
GTCCTACAGTGTGCATTTCTCATTT34680.14042288018340815No Hit
CTGAAGGACCTGGAATATGGCGAGA30880.12503629008257336No Hit
CTGTAGGACCTGGAATATGGCGAGA25490.10321162675533663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG1202.1827873E-1114.160991
GGTATCA12850.013.2242711
TTGGCGA604.298593E-412.59346817
TAGGACC12350.012.5422284
GCGTAAA655.2331726E-412.302761
ACCGTCC907.96635E-611.5435468
GTCCTAA19250.011.2162831
ACGGCAA600.00613633111.01928417
GACCGTC600.0061381811.0188397
ACCGTCT600.0061381811.0188398
GGCGAGG16750.010.99601219
CCTATAC2700.010.8441563
ACCGAGC700.001566352210.7939648
CCACGCA700.001566352210.7939649
GACTGCG803.9813243E-410.6253097
CCACCTT9000.010.59907313
ACGAAAT3050.010.52853912
CACCTTT9550.010.38424614
TTAGGAC23700.010.2419493
GTATTAG2051.4551915E-1110.2397981
Produced by FastQC (version 0.11.5)