##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511789_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2469683 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.260153631053054 31.0 30.0 34.0 24.0 34.0 2 26.514987551033876 31.0 26.0 34.0 10.0 34.0 3 28.26067394074462 31.0 28.0 34.0 16.0 34.0 4 32.62014558143697 35.0 32.0 37.0 26.0 37.0 5 32.990955114482304 35.0 35.0 37.0 27.0 37.0 6 33.192214547373084 37.0 35.0 37.0 27.0 37.0 7 33.169172723786815 37.0 35.0 37.0 26.0 37.0 8 33.18422040399517 37.0 35.0 37.0 26.0 37.0 9 34.71002432295967 39.0 35.0 39.0 27.0 39.0 10 34.58109846486371 39.0 35.0 39.0 27.0 39.0 11 34.57264960725729 39.0 35.0 39.0 27.0 39.0 12 34.45592491020103 39.0 35.0 39.0 27.0 39.0 13 34.45001119576885 39.0 35.0 39.0 27.0 39.0 14 35.517570068709226 40.0 36.0 41.0 26.0 41.0 15 35.46568162796602 40.0 36.0 41.0 26.0 41.0 16 35.41909224787149 40.0 36.0 41.0 26.0 41.0 17 35.372926403914995 40.0 36.0 41.0 26.0 41.0 18 35.34336147594651 40.0 36.0 41.0 25.0 41.0 19 35.36720907096174 40.0 36.0 41.0 25.0 41.0 20 35.30793506697013 40.0 36.0 41.0 25.0 41.0 21 35.19216474341039 40.0 36.0 41.0 25.0 41.0 22 35.090761850812434 40.0 36.0 41.0 24.0 41.0 23 34.971830797717764 40.0 35.0 41.0 24.0 41.0 24 34.86214991964556 40.0 35.0 41.0 22.0 41.0 25 34.756832354597734 39.0 35.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7238184204451841 1101 2 3.2513932555840945 1101 3 1.7993706585067848 1101 4 0.9371989332849822 1101 5 0.21542708298583335 1101 6 0.3288003050020123 1101 7 0.08897754746630682 1101 8 0.10970605229643127 1101 9 0.161201705202636 1101 10 0.3379727958088097 1101 11 0.16077840517696984 1101 12 0.17476142846415144 1101 13 0.22655928800524094 1101 14 0.10031854683833785 1101 15 0.18203163571803316 1101 16 0.1374933658649553 1101 17 0.11737714810789868 1101 18 0.1672551596982217 1101 19 0.2473370017047003 1101 20 0.2635175524261726 1101 21 0.27955049266868315 1101 22 0.3916470319989216 1101 23 0.26398628474984065 1101 24 0.4239563669209474 1101 25 0.29055282426662643 1102 1 1.6331401016769753 1102 2 4.213388677972773 1102 3 2.5907928391143606 1102 4 1.7408402150161564 1102 5 1.3683765211880754 1102 6 1.3086640170286472 1102 7 1.174552869979827 1102 8 1.0514871592460722 1102 9 1.1142031617153307 1102 10 1.1688820758753948 1102 11 1.2274450718436398 1102 12 1.2927597638782231 1102 13 1.384310016261587 1102 14 1.52625487642635 1102 15 1.3929138662631857 1102 16 1.3666444270384872 1102 17 1.4660922958398999 1102 18 1.4371261542883147 1102 19 1.4982109093201856 1102 20 1.512669653965915 1102 21 1.5471846999096357 1102 22 1.636023852639788 1102 23 1.5204261515829671 1102 24 1.3159611526055102 1102 25 1.5441316840252597 1103 1 1.2650107708337188 1103 2 3.7542593369242887 1103 3 2.3444042138605035 1103 4 1.4279977020810293 1103 5 0.9149161838032711 1103 6 0.6782641762301864 1103 7 0.8575376948071067 1103 8 0.7445254084373119 1103 9 0.7572192145375851 1103 10 0.9030550442698058 1103 11 0.9578038366029489 1103 12 0.7992719744324646 1103 13 0.7636064168779839 1103 14 1.10013689379565 1103 15 0.8030453606145898 1103 16 0.8781533719200567 1103 17 0.9324329055001712 1103 18 1.039971881869981 1103 19 0.928755409213025 1103 20 1.012686237459981 1103 21 1.0529761662987198 1103 22 0.9917921021371754 1103 23 1.1660879095353849 1103 24 1.3048310767806726 1103 25 1.2036419351759022 1104 1 1.1824163040430662 1104 2 3.764091668282507 1104 3 2.076200464503259 1104 4 1.3630807675001506 1104 5 1.1880240847495003 1104 6 0.9522791409808491 1104 7 0.7869272829160394 1104 8 0.81698118457156 1104 9 0.9505535570544907 1104 10 0.9066676811703616 1104 11 0.8418630612616269 1104 12 0.9015250968944883 1104 13 1.0388167835960687 1104 14 1.257461403981189 1104 15 1.1275783729314313 1104 16 1.0618319902035793 1104 17 1.0744538676846176 1104 18 1.1328636253066833 1104 19 1.079391675426045 1104 20 1.1886277817030688 1104 21 1.0963494344676263 1104 22 1.091404527589745 1104 23 1.0960409584711783 1104 24 1.131209453340702 1104 25 1.0020166690638064 1105 1 1.5240345100680521 1105 2 4.166666808145781 1105 3 2.6380030164188035 1105 4 1.8105064324399436 1105 5 1.3738962268363295 1105 6 1.098385388782006 1105 7 1.0912549389386115 1105 8 0.8925021795840848 1105 9 1.1221576905230322 1105 10 1.0350803335943155 1105 11 1.1751308606311213 1105 12 0.8804823710495029 1105 13 1.138124308044837 1105 14 1.242126456442854 1105 15 1.301768223674955 1105 16 1.3179076784826549 1105 17 1.1293713293817405 1105 18 1.314642768950236 1105 19 1.1347374073254457 1105 20 1.3686415323382946 1105 21 1.6071073520052082 1105 22 1.3594068224281983 1105 23 1.4458359636334137 1105 24 1.3727751172227514 1105 25 1.461169464374791 1106 1 0.9469129952514272 1106 2 3.648386005325154 1106 3 1.9560945593603876 1106 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-1.680017584554875 2315 16 -1.6816221312110713 2315 17 -1.6375692456542836 2315 18 -1.680649323732581 2315 19 -1.783072355020444 2315 20 -1.5603615898740202 2315 21 -1.8688413201968146 2315 22 -1.7576707067925028 2315 23 -1.790740810766728 2315 24 -1.8447327168997134 2315 25 -1.8866279749536474 2316 1 -1.3867875015782545 2316 2 -1.2818644670710597 2316 3 -0.6983657468152309 2316 4 -0.9469755688430901 2316 5 -1.2086193992883416 2316 6 -1.2840338454144593 2316 7 -1.327426463558222 2316 8 -1.4694452178410131 2316 9 -1.5326566040450373 2316 10 -1.7636089307976164 2316 11 -1.6291156788972216 2316 12 -1.679315755403941 2316 13 -1.5041374278083666 2316 14 -1.71360967179848 2316 15 -2.180974315574062 2316 16 -1.9583834131201527 2316 17 -2.00620042600886 2316 18 -1.8267600112184965 2316 19 -1.8324575689607911 2316 20 -1.9365380884962775 2316 21 -2.097269651479415 2316 22 -2.0038673950062886 2316 23 -1.8872817185184942 2316 24 -1.922981248171915 2316 25 -1.9301169399987899 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 102497.0 3 3.0 4 1007.0 5 4807.0 6 5485.0 7 4908.0 8 4516.0 9 4348.0 10 4194.0 11 4052.0 12 3931.0 13 3886.0 14 3965.0 15 4029.0 16 4321.0 17 4507.0 18 5084.0 19 6576.0 20 8410.0 21 10744.0 22 13536.0 23 16121.0 24 18312.0 25 21144.0 26 23409.0 27 26214.0 28 30221.0 29 36249.0 30 45067.0 31 56244.0 32 71710.0 33 93790.0 34 126313.0 35 179154.0 36 276796.0 37 370295.0 38 873838.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.458364089642274 19.54975598082831 12.93943392735019 25.05244600217923 2 15.398895254909858 21.343478018658107 41.23150366177145 22.026123064660585 3 18.18881208640947 25.546315053389446 30.03450240374979 26.230370456451297 4 11.789610245525438 17.39644318724306 39.70137058075874 31.112575986472756 5 12.296653158800035 38.589213466403635 36.709125746252944 12.405007628543386 6 30.287117142725258 38.47883144764408 18.82502569185805 12.40902571777261 7 27.00374906415115 31.89437672770149 23.44171296478131 17.66016124336605 8 23.75705707979526 38.78222427736677 21.20276164997694 16.25795699286103 9 26.13963006588295 15.305122155353542 21.309576978098 37.24567080066551 10 14.515790083180717 29.43859596555509 35.79090919765816 20.254704753606028 11 33.15757528395344 22.42502377835536 26.330788202372528 18.086612735318663 12 23.25557571558779 24.866916118384424 32.99601608789468 18.881492078133107 13 28.74696316931748 22.86778044119076 26.227972854782806 22.15728353470895 14 19.740104296786267 23.54399329792528 28.608732375774544 28.107170029513913 15 21.691164412598702 33.334156650873815 23.64307483996934 21.33160409655814 16 20.54421559366121 28.837101765692196 29.92606743456549 20.692615206081104 17 20.530695888467374 29.865208330043412 29.066759310885736 20.537336470603478 18 20.260899880672945 27.74947230069608 32.180850740763084 19.80877707786789 19 22.7954562387232 27.31289823102015 28.469246118512903 21.422399411743744 20 22.871392325925854 28.08837308364775 29.4253554934149 19.6148790970115 21 21.741026490307565 27.399097364898296 28.1110178008453 22.74885834394884 22 22.326477508919538 29.57747049193405 28.243680400586495 19.852371598559916 23 20.74464229821891 29.23905997119452 29.25930559925237 20.756992131334197 24 22.39424888536521 27.301719317326434 30.13832363625026 20.165708161058095 25 21.517571173476618 28.462952540217923 29.435106714662286 20.584369571643172 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 248.0 1 248.0 2 438.0 3 628.0 4 628.0 5 628.0 6 1740.5 7 2853.0 8 2853.0 9 2853.0 10 4281.5 11 5710.0 12 5710.0 13 5710.0 14 9561.5 15 13413.0 16 13413.0 17 13413.0 18 24002.0 19 34591.0 20 34591.0 21 34591.0 22 55272.5 23 75954.0 24 75954.0 25 75954.0 26 109321.0 27 142688.0 28 142688.0 29 142688.0 30 184809.0 31 226930.0 32 226930.0 33 226930.0 34 252823.0 35 278716.0 36 278716.0 37 278716.0 38 291341.0 39 303966.0 40 303966.0 41 303966.0 42 312541.0 43 321116.0 44 321116.0 45 321116.0 46 319569.5 47 318023.0 48 318023.0 49 318023.0 50 295880.0 51 273737.0 52 273737.0 53 273737.0 54 233958.0 55 194179.0 56 194179.0 57 194179.0 58 162446.5 59 130714.0 60 130714.0 61 130714.0 62 104559.0 63 78404.0 64 78404.0 65 78404.0 66 59208.0 67 40012.0 68 40012.0 69 40012.0 70 28845.5 71 17679.0 72 17679.0 73 17679.0 74 12228.0 75 6777.0 76 6777.0 77 6777.0 78 4553.5 79 2330.0 80 2330.0 81 2330.0 82 1533.0 83 736.0 84 736.0 85 736.0 86 458.0 87 180.0 88 180.0 89 180.0 90 124.0 91 68.0 92 68.0 93 68.0 94 44.0 95 20.0 96 20.0 97 20.0 98 15.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.511355101039283 3 0.0 4 0.0 5 4.4540129239258643E-4 6 0.0011742397708531824 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.2147307974343265E-4 14 0.0 15 0.0 16 0.0 17 8.098205316228844E-4 18 0.0 19 4.4540129239258643E-4 20 6.883474518794517E-4 21 0.0010122756645286053 22 0.004211066764438999 23 5.66874372136019E-4 24 0.0016196410632457689 25 5.263833455548749E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2469683.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.95035995450814 #Duplication Level Percentage of deduplicated Percentage of total 1 86.35809834261141 63.86212457422927 2 9.957073646881007 14.726583605607951 3 2.0642719725337755 4.579609662386258 4 0.6500113995642964 1.922743078892534 5 0.2967521280840204 1.097246334453981 6 0.15964363639045043 0.7083422625312255 7 0.09287133934034174 0.4807508281572881 8 0.06559960861759319 0.38808917361166967 9 0.05004153248645887 0.33305304060439617 >10 0.26307636529922884 3.5661797419169092 >50 0.02163673169097662 1.1115844434370632 >100 0.016811096567576397 2.5389300329125275 >500 0.0020005296970794016 0.9678967381657898 >1k 0.0018338188889737597 2.394571516687436 >5k 2.77851346814206E-4 1.322294966405554 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 7398 0.2995526146473049 No Hit CTGTAGGACGTGGAATATGGCAAGA 6685 0.27068251269494914 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6473 0.2620984150597465 No Hit GTATCAACGCAGAGTACTTTTTTTT 5990 0.24254124922105388 No Hit CTTTAGGACGTGAAATATGGCGAGG 5631 0.22800497067842312 No Hit GGTATCAACGCAGAGTACTTTTTTT 3820 0.15467572153997092 No Hit TATCAACGCAGAGTACTTTTTTTTT 3623 0.1466989893034855 No Hit GTCCTACAGTGTGCATTTCTCATTT 3468 0.14042288018340815 No Hit CTGAAGGACCTGGAATATGGCGAGA 3088 0.12503629008257336 No Hit CTGTAGGACCTGGAATATGGCGAGA 2549 0.10321162675533663 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 4.0491026581144216E-5 10 0.0 0.0 0.0 0.0 4.0491026581144216E-5 11 0.0 0.0 0.0 0.0 4.0491026581144216E-5 12 0.0 0.0 0.0 0.0 8.098205316228843E-5 13 0.0 0.0 0.0 4.0491026581144216E-5 8.098205316228843E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 120 2.1827873E-11 14.16099 1 GGTATCA 1285 0.0 13.224271 1 TTGGCGA 60 4.298593E-4 12.593468 17 TAGGACC 1235 0.0 12.542228 4 GCGTAAA 65 5.2331726E-4 12.30276 1 ACCGTCC 90 7.96635E-6 11.543546 8 GTCCTAA 1925 0.0 11.216283 1 ACGGCAA 60 0.006136331 11.019284 17 GACCGTC 60 0.00613818 11.018839 7 ACCGTCT 60 0.00613818 11.018839 8 GGCGAGG 1675 0.0 10.996012 19 CCTATAC 270 0.0 10.844156 3 ACCGAGC 70 0.0015663522 10.793964 8 CCACGCA 70 0.0015663522 10.793964 9 GACTGCG 80 3.9813243E-4 10.625309 7 CCACCTT 900 0.0 10.599073 13 ACGAAAT 305 0.0 10.528539 12 CACCTTT 955 0.0 10.384246 14 TTAGGAC 2370 0.0 10.241949 3 GTATTAG 205 1.4551915E-11 10.239798 1 >>END_MODULE