FastQCFastQC Report
Thu 26 May 2016
SRR1511789_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511789_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469683
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT81640.3305687410084614No Hit
GTATCAACGCAGAGTACTTTTTTTT76450.30955389821284757No Hit
CTGTAGGACGTGGAATATGGCAAGA74860.3031158249864456No Hit
GTCCTAAAGTGTGTATTTCTCATTT70610.28590713868945933No Hit
CTTTAGGACGTGAAATATGGCGAGG64790.2623413612192334No Hit
GGTATCAACGCAGAGTACTTTTTTT50340.20383182780948No Hit
TATCAACGCAGAGTACTTTTTTTTT48450.19617902378564372No Hit
GTCCTACAGTGTGCATTTCTCATTT40470.16386718457389066No Hit
CTGAAGGACCTGGAATATGGCGAGA34750.14070631736947614No Hit
CTGTAGGACCTGGAATATGGCGAGA30570.12378106825855788No Hit
TTTCTAAATTTTCCACCTTTTTCAG27490.11130983207156546No Hit
GTCCTTCAGTGTGCATTTCTCATTT27350.11074295769942943No Hit
ATTTAGAAATGTCCACTGTAGGACG26510.10734171146661332No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25480.10317113572875546No Hit
GAATATGGCAAGAAAACTGAAAATC25270.10232082417055144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14450.013.3987341
TAGGACC11000.012.868254
GGCGAGG17100.012.274363519
AATCCCG1751.8189894E-1211.3968319
TGTCGAG1604.5474735E-1111.2783418
CGTGCGC855.340684E-511.17336210
CCACCTT10400.011.14113113
GACTGCG600.005890387611.0800277
AGTAGCG700.001495659510.8539047
TATGTCG1502.1445885E-910.76367316
TGTAGGA40950.010.7412052
CACCTTT10750.010.69004914
ACCTTTT10450.010.63340615
AGGACCT25150.010.614375
CTGTAGG40150.010.5708531
AGTCGTC3150.010.5524068
TGGCGAG41600.010.52492918
GGACGAG1451.45137165E-810.479636
GGACGTG39800.010.4516546
AGGACGT40550.010.4488845
Produced by FastQC (version 0.11.5)