FastQCFastQC Report
Thu 26 May 2016
SRR1511788_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511788_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3266685
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT74020.22659056505295122No Hit
CTGTAGGACGTGGAATATGGCAAGA67870.20776414009921373No Hit
GTCCTAAAGTGTGTATTTCTCATTT56070.17164189384651413No Hit
GTATCAACGCAGAGTACTTTTTTTT55750.17066230750745787No Hit
CTTTAGGACGTGAAATATGGCGAGG50480.1545297449861251No Hit
GTCCTACAGTGTGCATTTCTCATTT40920.1252646031068193No Hit
GGTATCAACGCAGAGTACTTTTTTT34860.10671368681094137No Hit
TATCAACGCAGAGTACTTTTTTTTT34230.10478512620592435No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG250.006238954618.8901335
AATTTCG400.005487277614.16781617
GGTATCA14600.013.347611
GTTTGCG500.0015671613.2230929
CGTTAGG852.238201E-612.9328871
TAGGACC13750.012.5705244
CGGTAGG1507.2759576E-1111.9923131
TCCAACG2900.011.39956818
CGTAGGA1103.3016295E-610.9021032
AATGCCG4050.010.72805818
TGTAGGA39800.010.7217712
GGCCTAC2900.010.6828091
TCGAACT2150.010.54365319
AGTACCG1002.559194E-510.3895735
GGACGTG37400.010.3037096
AGGACGT37950.010.278825
TGTAGAA13600.010.2140422
CTGTAGG39500.010.1959961
AGGACCT26300.010.16332155
GTAGGAC41700.010.1472173
Produced by FastQC (version 0.11.5)