FastQCFastQC Report
Thu 26 May 2016
SRR1511788_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511788_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3266685
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT84380.25830467277989766No Hit
CTGTAGGACGTGGAATATGGCAAGA74290.22741709102652996No Hit
GTATCAACGCAGAGTACTTTTTTTT69440.21257023557520852No Hit
GTCCTAAAGTGTGTATTTCTCATTT62940.1926723880631282No Hit
CTTTAGGACGTGAAATATGGCGAGG57760.176815334199655No Hit
GTCCTACAGTGTGCATTTCTCATTT45740.14001962233885423No Hit
GGTATCAACGCAGAGTACTTTTTTT45290.13864207904955636No Hit
TATCAACGCAGAGTACTTTTTTTTT43020.131693138456876No Hit
CTGTAGGACCTGGAATATGGCGAGA35690.10925448887786854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA2700.014.77350419
TCCAACG4150.014.41751518
TAGGACC15350.013.24513154
GGTATCA17350.012.4241181
CGGTTTC2900.012.11699913
ATTGCGA550.003076699612.08722717
AGGCCGT658.048112E-411.6887466
CCGGTTT3000.011.39652812
GGCGAGG15650.011.2874719
GCCGGTT3000.011.07995811
TGTAGGA43800.010.9105022
GTCCTAA19350.010.8935121
TAAGACG1053.4668137E-610.8578254
ATCCCGT2700.010.55234110
CTGTAGG43800.010.5179141
AGGACCT28850.010.5049935
GTCCTAC40600.010.501181
GTAGGAC43950.010.4841633
GGACGTG39500.010.2664936
AGGACGT40700.010.224235
Produced by FastQC (version 0.11.5)