FastQCFastQC Report
Thu 26 May 2016
SRR1511787_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511787_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249712
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69800.3102619357499982No Hit
GTCCTACAGTGGACATTTCTAAATT47740.21220494001009904No Hit
CTGTAGGACGTGGAATATGGCAAGA43740.19442488638545732No Hit
GGTATCAACGCAGAGTACTTTTTTT42360.18829076788495594No Hit
GTCCTAAAGTGTGTATTTCTCATTT39900.17735603490580126No Hit
TATCAACGCAGAGTACTTTTTTTTT39610.17606698101801474No Hit
CTTTAGGACGTGAAATATGGCGAGG38420.17077741506468383No Hit
GTCCTACAGTGTGCATTTCTCATTT24290.10796937563563691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15050.013.8151361
TAGGACC8700.013.1362294
CCGTCCA658.42908E-411.6241689
GGCGAGG12950.011.52369919
GTGGTAT4550.011.4240551
GTCCTAA12600.010.71005251
TGTAGGA28000.010.6743512
ACTGTTC6200.010.66330058
GTAGGAC28700.010.4981643
AGGACCT17150.010.4639115
AGGACGT30400.010.4392745
GTATAGG2400.010.412551
GACGTGG15450.010.3921587
GGACGTG29800.010.3642066
TGGCGAG27850.010.34377918
CTGTAGG28500.010.2064911
GTATCAA30450.010.0781171
AGAACCG750.002778862410.0747285
GGACGTC750.002778862410.0747286
TGCGCCA1502.6780981E-810.07427912
Produced by FastQC (version 0.11.5)