##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511787_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2249712 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.821379803281488 31.0 30.0 34.0 25.0 34.0 2 27.017271544090978 31.0 27.0 34.0 10.0 34.0 3 28.80143102761598 31.0 28.0 34.0 16.0 34.0 4 33.23287691935679 35.0 33.0 37.0 28.0 37.0 5 33.611071105990455 35.0 35.0 37.0 28.0 37.0 6 33.8260804049585 37.0 35.0 37.0 30.0 37.0 7 33.822973340587595 37.0 35.0 37.0 29.0 37.0 8 33.845818486988556 37.0 35.0 37.0 30.0 37.0 9 35.42591007204478 39.0 35.0 39.0 30.0 39.0 10 35.3116758945145 39.0 35.0 39.0 30.0 39.0 11 35.308291016805704 39.0 35.0 39.0 30.0 39.0 12 35.199869583306665 39.0 35.0 39.0 29.0 39.0 13 35.194488894578505 39.0 35.0 39.0 29.0 39.0 14 36.31697968451073 40.0 36.0 41.0 29.0 41.0 15 36.25538780074961 40.0 36.0 41.0 29.0 41.0 16 36.21642103522584 40.0 36.0 41.0 28.0 41.0 17 36.176946204669754 40.0 36.0 41.0 28.0 41.0 18 36.15009610118984 40.0 36.0 41.0 28.0 41.0 19 36.17944785821474 40.0 36.0 41.0 28.0 41.0 20 36.112132130690505 40.0 36.0 41.0 28.0 41.0 21 36.012563830392516 40.0 36.0 41.0 27.0 41.0 22 35.91899407568613 40.0 36.0 41.0 27.0 41.0 23 35.81064865191633 40.0 36.0 41.0 27.0 41.0 24 35.70840445354783 40.0 36.0 41.0 27.0 41.0 25 35.60338212180048 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6016458571561323 1101 2 3.237242921658904 1101 3 1.818640112477535 1101 4 0.874981900681334 1101 5 0.29875555467429393 1101 6 0.3423788104267089 1101 7 0.18641909311777738 1101 8 0.12062180314205762 1101 9 0.13036541200180807 1101 10 0.06535892002609955 1101 11 0.14098829501164545 1101 12 0.22469950079741352 1101 13 0.1282099244967938 1101 14 0.18931190321649893 1101 15 0.1553873770497134 1101 16 0.13271156600104916 1101 17 0.1840247725020845 1101 18 0.21654322353115418 1101 19 0.25279808007047677 1101 20 0.18192927048208674 1101 21 0.1834912105449149 1101 22 0.12683536312972876 1101 23 -0.007013391469804731 1101 24 0.10117804988720991 1101 25 0.3227902805012022 1102 1 1.0497938241267448 1102 2 3.8425965004950484 1102 3 2.0424983678682906 1102 4 1.1226455800927582 1102 5 0.82996421901899 1102 6 0.6166460197563381 1102 7 0.528201858350819 1102 8 0.4980872670797609 1102 9 0.6558448077674726 1102 10 0.6050127767161158 1102 11 0.563391731007421 1102 12 0.5310441570021638 1102 13 0.5982946503606854 1102 14 0.7079114805655422 1102 15 0.5432811664286561 1102 16 0.647203015145962 1102 17 0.5828771577406044 1102 18 0.592165459667811 1102 19 0.7834402747247253 1102 20 0.7271607501517963 1102 21 0.6726255587623413 1102 22 0.8094147080207392 1102 23 0.8568969126040855 1102 24 0.5925332723659764 1102 25 0.6879245113155861 1103 1 0.959675341792039 1103 2 3.593373371712538 1103 3 1.9873698809481475 1103 4 1.0168688794079728 1103 5 0.7497356862444988 1103 6 0.5717305471344218 1103 7 0.6323333921219785 1103 8 0.4331034108426195 1103 9 0.5152694872607242 1103 10 0.6282928596329072 1103 11 0.6347970910398573 1103 12 0.6281923764512314 1103 13 0.7041684599476525 1103 14 0.8610048491170375 1103 15 0.5353495007788638 1103 16 0.510207460365173 1103 17 0.6771092679123143 1103 18 0.7900236636173474 1103 19 0.7309029375360936 1103 20 0.859326439729216 1103 21 0.7787120605302036 1103 22 0.7531152298508204 1103 23 0.8017826367428 1103 24 0.8568343345952556 1103 25 0.9376142365305498 1104 1 1.1999347941196739 1104 2 3.8093675928329596 1104 3 2.1937960565759553 1104 4 1.3742571986689 1104 5 1.1161773723633814 1104 6 0.9205485056406886 1104 7 0.8104237375652303 1104 8 0.8312187594870579 1104 9 1.0618731286064076 1104 10 0.9983085646838177 1104 11 0.8508173288824707 1104 12 0.9517071496510212 1104 13 0.9671312352215438 1104 14 1.085360618517477 1104 15 1.052462169474687 1104 16 1.0985844557912117 1104 17 1.0086408112649963 1104 18 0.9607245812610756 1104 19 1.0229680182535361 1104 20 1.2390674217677216 1104 21 1.1421726827506546 1104 22 1.0662547604729582 1104 23 1.1141394033755176 1104 24 1.196717698236185 1104 25 1.1117398597441621 1105 1 1.133997663765367 1105 2 3.699678859130202 1105 3 2.097410846693812 1105 4 1.2897424583697585 1105 5 0.868169026633737 1105 6 0.7509930391965298 1105 7 0.5937116591090685 1105 8 0.5179610268603483 1105 9 0.6500268679433177 1105 10 0.5917131881059703 1105 11 0.6512456472774844 1105 12 0.4623682676741012 1105 13 0.6399680390837617 1105 14 0.7709206763387115 1105 15 0.8262125207885802 1105 16 0.7812475420476304 1105 17 0.7751313321376401 1105 18 0.8672017587724312 1105 19 0.7387318775318548 1105 20 0.6307462950416891 1105 21 0.8538387555034888 1105 22 0.7378651395767619 1105 23 0.8078527890257945 1105 24 0.8252508653320163 1105 25 0.9526962302798481 1106 1 0.8324787991788938 1106 2 3.573146749175862 1106 3 1.9703320727936458 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0.6265660776920754 1107 17 0.6627095210490737 1107 18 0.7331530495272816 1107 19 0.7072526328296291 1107 20 0.7920065856978269 1107 21 0.7294299297549784 1107 22 0.8068441124266599 1107 23 0.6857032652274242 1107 24 0.7255680542618563 1107 25 0.7432448868274761 1108 1 0.9212508565716853 1108 2 3.735375500548624 1108 3 1.973296002202929 1108 4 1.231063411762051 1108 5 0.7417389260328591 1108 6 0.753418825665662 1108 7 0.6650090137626634 1108 8 0.4858772071258315 1108 9 0.347600797542988 1108 10 0.45749505755765085 1108 11 0.5261212245704527 1108 12 0.3813570183200454 1108 13 0.4590523528585635 1108 14 0.553037350968971 1108 15 0.5305411735837211 1108 16 0.567155712208411 1108 17 0.4909792764352048 1108 18 0.6777655001094942 1108 19 0.6248233455616088 1108 20 0.6135485823914166 1108 21 0.5896034592861881 1108 22 0.5161377914049154 1108 23 0.5347486990374577 1108 24 0.5225043582941709 1108 25 0.41116581678679154 1109 1 0.7804691168405924 1109 2 2.7768570717531595 1109 3 1.5218778890853848 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2314 1 0.22940264046805225 2314 2 2.3812683165648068 2314 3 1.087863905085804 2314 4 0.3512006294244401 2314 5 0.02049238484586624 2314 6 0.06318978465390757 2314 7 -0.14669253865609022 2314 8 -0.3299450474361265 2314 9 -0.19652597942741323 2314 10 -0.263301045207065 2314 11 -0.2919570605926154 2314 12 -0.2832250702856456 2314 13 -0.37800275784014303 2314 14 -0.8813630268719308 2314 15 -0.5921109223985539 2314 16 -0.5277449376645862 2314 17 -0.46269883115599697 2314 18 -0.3292923142210924 2314 19 -0.4479766157197105 2314 20 -0.3740717876389894 2314 21 -0.21452859143528258 2314 22 -0.08572604568290387 2314 23 -0.2531323545111448 2314 24 -0.06391907051185797 2314 25 -0.12539579764860065 2315 1 -4.413834339004676 2315 2 -13.955254373508089 2315 3 -7.075436396187225 2315 4 -3.283914304372889 2315 5 -2.243963704108509 2315 6 -1.7064267873692671 2315 7 -1.4625644101893727 2315 8 -1.3805391372103273 2315 9 -1.4432638604260006 2315 10 -1.4677993582500903 2315 11 -1.4761400832411269 2315 12 -1.6678334870113147 2315 13 -1.501502340759025 2315 14 -1.7151111958714793 2315 15 -1.7871220015637022 2315 16 -1.5782689406113874 2315 17 -1.5553427241424345 2315 18 -1.5576965417897242 2315 19 -1.6339149622438924 2315 20 -1.6233002592301773 2315 21 -1.8703866462606697 2315 22 -1.7135136811626097 2315 23 -1.6284445920438628 2315 24 -1.6454038219687703 2315 25 -1.6974526525416351 2316 1 -1.1276973885871264 2316 2 -1.1588634882900486 2316 3 -0.5346113026293757 2316 4 -0.791240915371894 2316 5 -0.7784607482708736 2316 6 -0.92570277281812 2316 7 -1.012601276047171 2316 8 -0.9641294920566637 2316 9 -0.9953225374159729 2316 10 -1.242146380676978 2316 11 -1.2436920069285833 2316 12 -1.1926245672495313 2316 13 -1.0809103300892176 2316 14 -1.3151035729331895 2316 15 -1.7332733056275487 2316 16 -1.3707358894711419 2316 17 -1.3354802921912352 2316 18 -1.3793127115433208 2316 19 -1.3895024821656747 2316 20 -1.4170050693186766 2316 21 -1.4782156348801152 2316 22 -1.3367281558519224 2316 23 -1.487392225200928 2316 24 -1.3567010598230738 2316 25 -1.4835911188844122 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68178.0 3 1.0 4 614.0 5 3171.0 6 3839.0 7 3501.0 8 3193.0 9 3057.0 10 3037.0 11 2951.0 12 2967.0 13 2945.0 14 2951.0 15 2956.0 16 3179.0 17 3441.0 18 3909.0 19 4834.0 20 6234.0 21 7858.0 22 9918.0 23 11613.0 24 13831.0 25 15776.0 26 18177.0 27 20824.0 28 24318.0 29 29875.0 30 37640.0 31 48159.0 32 62987.0 33 84005.0 34 115663.0 35 167603.0 36 262515.0 37 354852.0 38 839140.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.580561422973254 19.282912657264575 13.307525585497165 24.829000334265007 2 15.752962595322886 21.177322914657495 40.803793627421 22.265920862598616 3 18.499656844965045 25.25732182608263 29.966991330445854 26.276029998506477 4 12.059099120242948 17.171975790678985 39.221020290597195 31.547904798480875 5 12.816397179362262 38.194402463612995 35.9866898045254 13.002510552499352 6 30.689849068429275 37.78169929701582 18.626347127055002 12.902104507499907 7 27.226640565547942 31.894349143357015 22.870749678181028 18.00826061291401 8 24.292487216141446 37.733718804895915 20.930367975989817 17.043426002972826 9 26.134100720447773 15.56572574622885 20.881339478119866 37.41883405520351 10 14.935511745503424 29.046962455638763 34.91544695498801 21.102078843869794 11 33.38996280412781 22.597648054506532 25.6810649540919 18.331324187273747 12 23.44260065288357 24.60830541864914 32.346851508104145 19.60224242036314 13 28.685640066657452 22.45101704174447 26.254038852101445 22.609304039496628 14 20.562632016898164 22.873994537967526 28.067814902529747 28.495558542604567 15 22.286008164600624 31.964935956246844 23.806202749507495 21.94285312964504 16 21.375536068616782 28.574057479357357 28.71011933971993 21.34028711230593 17 21.02865885586828 29.1703004748176 28.538084531934395 21.262956137379728 18 20.82155404780701 27.525612167246294 31.146031136429908 20.50680264851679 19 23.120733890798274 27.020173284710726 28.22775222384416 21.63134060064684 20 23.11311927563836 27.58397212078917 28.97366543613496 20.329243167437507 21 22.377630459500857 27.011177480395183 27.80470480090252 22.80648725920144 22 22.673989336047846 28.56290020381084 28.058907333856382 20.70420312628493 23 21.69139283853727 28.242776395870038 28.602511970442386 21.463318795150304 24 22.68177706458517 26.96490366343433 29.3802410581131 20.9730782138674 25 22.168086560951735 27.666380109685836 28.92992748360002 21.23560584576241 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 253.0 1 253.0 2 450.0 3 647.0 4 647.0 5 647.0 6 1687.5 7 2728.0 8 2728.0 9 2728.0 10 4086.5 11 5445.0 12 5445.0 13 5445.0 14 8719.0 15 11993.0 16 11993.0 17 11993.0 18 21177.0 19 30361.0 20 30361.0 21 30361.0 22 47488.5 23 64616.0 24 64616.0 25 64616.0 26 92847.0 27 121078.0 28 121078.0 29 121078.0 30 155834.0 31 190590.0 32 190590.0 33 190590.0 34 212045.5 35 233501.0 36 233501.0 37 233501.0 38 246734.5 39 259968.0 40 259968.0 41 259968.0 42 270497.0 43 281026.0 44 281026.0 45 281026.0 46 284624.0 47 288222.0 48 288222.0 49 288222.0 50 274949.0 51 261676.0 52 261676.0 53 261676.0 54 229209.0 55 196742.0 56 196742.0 57 196742.0 58 167145.0 59 137548.0 60 137548.0 61 137548.0 62 111156.5 63 84765.0 64 84765.0 65 84765.0 66 65174.5 67 45584.0 68 45584.0 69 45584.0 70 33224.5 71 20865.0 72 20865.0 73 20865.0 74 14428.5 75 7992.0 76 7992.0 77 7992.0 78 5413.0 79 2834.0 80 2834.0 81 2834.0 82 1859.5 83 885.0 84 885.0 85 885.0 86 585.5 87 286.0 88 286.0 89 286.0 90 177.0 91 68.0 92 68.0 93 68.0 94 49.0 95 30.0 96 30.0 97 30.0 98 19.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.5136835292695245 3 0.0 4 0.0 5 3.5560107249283464E-4 6 7.556522790472736E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 4.445013406160433E-5 14 0.0 15 0.0 16 0.0 17 2.66700804369626E-4 18 0.0 19 1.7780053624641732E-4 20 3.111509384312303E-4 21 4.4450134061604327E-4 22 0.0034226603227435334 23 1.7780053624641732E-4 24 0.0020891563008954036 25 6.223018768624606E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2249712.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.04524908977297 #Duplication Level Percentage of deduplicated Percentage of total 1 83.40678587514213 59.256558782768295 2 11.349829981759394 16.12702996361339 3 2.8586544989013003 6.092814628081291 4 0.9777565883121127 2.7785984146320257 5 0.44739994502398933 1.5892820268490027 6 0.2418432263848998 1.0309087355513735 7 0.1602074237290634 0.796738342740348 8 0.10006057277954317 0.5687062653750401 9 0.07127223965009112 0.4557198617214045 >10 0.34448433661226074 4.246752314112749 >50 0.022188081701969422 1.1010516417031107 >100 0.015718809500579405 2.221025325762872 >500 0.002279052301599436 1.1786573507140785 >1k 0.0014560611926876367 2.2422195262582436 >5k 6.330700837772334E-5 0.3139368201167942 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6980 0.3102619357499982 No Hit GTCCTACAGTGGACATTTCTAAATT 4774 0.21220494001009904 No Hit CTGTAGGACGTGGAATATGGCAAGA 4374 0.19442488638545732 No Hit GGTATCAACGCAGAGTACTTTTTTT 4236 0.18829076788495594 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3990 0.17735603490580126 No Hit TATCAACGCAGAGTACTTTTTTTTT 3961 0.17606698101801474 No Hit CTTTAGGACGTGAAATATGGCGAGG 3842 0.17077741506468383 No Hit GTCCTACAGTGTGCATTTCTCATTT 2429 0.10796937563563691 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 8.890026812320866E-5 0.0 6 0.0 0.0 0.0 8.890026812320866E-5 0.0 7 0.0 0.0 0.0 8.890026812320866E-5 0.0 8 0.0 0.0 0.0 8.890026812320866E-5 0.0 9 0.0 0.0 0.0 1.33350402184813E-4 0.0 10 0.0 0.0 0.0 1.33350402184813E-4 0.0 11 0.0 0.0 0.0 1.33350402184813E-4 0.0 12 0.0 0.0 0.0 1.33350402184813E-4 8.890026812320866E-5 13 0.0 0.0 0.0 1.7780053624641732E-4 8.890026812320866E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1505 0.0 13.815136 1 TAGGACC 870 0.0 13.136229 4 CCGTCCA 65 8.42908E-4 11.624168 9 GGCGAGG 1295 0.0 11.523699 19 GTGGTAT 455 0.0 11.424055 1 GTCCTAA 1260 0.0 10.7100525 1 TGTAGGA 2800 0.0 10.674351 2 ACTGTTC 620 0.0 10.6633005 8 GTAGGAC 2870 0.0 10.498164 3 AGGACCT 1715 0.0 10.463911 5 AGGACGT 3040 0.0 10.439274 5 GTATAGG 240 0.0 10.41255 1 GACGTGG 1545 0.0 10.392158 7 GGACGTG 2980 0.0 10.364206 6 TGGCGAG 2785 0.0 10.343779 18 CTGTAGG 2850 0.0 10.206491 1 GTATCAA 3045 0.0 10.078117 1 AGAACCG 75 0.0027788624 10.074728 5 GGACGTC 75 0.0027788624 10.074728 6 TGCGCCA 150 2.6780981E-8 10.074279 12 >>END_MODULE