FastQCFastQC Report
Thu 26 May 2016
SRR1511787_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511787_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249712
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85080.37818174059612963No Hit
GGTATCAACGCAGAGTACTTTTTTT55020.24456463760694705No Hit
TATCAACGCAGAGTACTTTTTTTTT54320.2414531282226347No Hit
GTCCTACAGTGGACATTTCTAAATT50020.22233957057614484No Hit
CTGTAGGACGTGGAATATGGCAAGA48270.21456079711536408No Hit
CTTTAGGACGTGAAATATGGCGAGG42780.1901576735155433No Hit
GTCCTAAAGTGTGTATTTCTCATTT42570.18922422070024963No Hit
GTCCTACAGTGTGCATTTCTCATTT26610.11828180673792911No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26130.11614820030297211No Hit
CTGAAGGACCTGGAATATGGCGAGA22690.10085735418578021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16900.013.2585691
GGCGCCC550.003074509312.08810818
TAGGACC8350.011.6046814
TCCAACG1657.2759576E-1211.51248418
ACTGCGT752.079929E-411.39685258
GTATTAC1701.0913936E-1111.2175131
GCGCCCA600.00588718611.08076719
GTCTAGA2350.010.9549761
GTGGTAT5050.010.9509581
AATCCCG2200.010.792953519
GGCGAGG12300.010.73328619
GGACCGT909.545176E-510.5526426
TTAGGAC16900.010.5117363
TGGTATC5250.010.3141892
CCTACAC4100.010.1950263
TAGGACA8550.09.9999074
TGTAGGA29450.09.96764852
TGGCGAG27650.09.96152618
CCCGTTT1151.0298958E-59.91052712
CCACCTT8400.09.83678813
Produced by FastQC (version 0.11.5)