##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511787_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2249712 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.927465826736935 31.0 31.0 34.0 28.0 34.0 2 31.123133983372092 31.0 31.0 34.0 28.0 34.0 3 31.23515143271672 33.0 31.0 34.0 27.0 34.0 4 34.82025832639911 37.0 35.0 37.0 32.0 37.0 5 34.469845473553946 37.0 35.0 37.0 31.0 37.0 6 34.41302575618568 37.0 35.0 37.0 30.0 37.0 7 34.25575184734757 37.0 35.0 37.0 30.0 37.0 8 34.17391114951603 37.0 35.0 37.0 30.0 37.0 9 35.675647371752476 39.0 35.0 39.0 30.0 39.0 10 35.48842251808232 39.0 35.0 39.0 30.0 39.0 11 35.462318732353296 39.0 35.0 39.0 30.0 39.0 12 35.300105969119606 39.0 35.0 39.0 30.0 39.0 13 35.286045502713236 39.0 35.0 39.0 30.0 39.0 14 36.304016247413 40.0 36.0 41.0 29.0 41.0 15 36.2404601122277 40.0 36.0 41.0 29.0 41.0 16 36.14339302097335 39.0 36.0 41.0 29.0 41.0 17 36.08661375322708 39.0 36.0 41.0 28.0 41.0 18 36.016295419146985 39.0 36.0 41.0 28.0 41.0 19 36.03350695555698 39.0 36.0 41.0 28.0 41.0 20 35.90193544773731 39.0 36.0 41.0 27.0 41.0 21 35.79210227798047 39.0 36.0 41.0 27.0 41.0 22 35.88388380379355 39.0 36.0 41.0 27.0 41.0 23 35.77800847397356 39.0 36.0 41.0 27.0 41.0 24 35.651541619549526 39.0 36.0 41.0 27.0 41.0 25 35.20034253273308 39.0 35.0 40.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33182453286868707 1101 2 0.24471964774214428 1101 3 0.32919486902878603 1101 4 0.2604987652183013 1101 5 0.37377590003126926 1101 6 0.4283514089667477 1101 7 0.3988826090526558 1101 8 0.355299456329881 1101 9 0.34585070054239964 1101 10 0.39646149228381944 1101 11 0.20718575675220308 1101 12 0.3440270149423412 1101 13 0.25059499486433623 1101 14 0.344809262946832 1101 15 0.10556321238492927 1101 16 0.40333985969598984 1101 17 0.31302206658718035 1101 18 0.3049586140236258 1101 19 0.36639032000121574 1101 20 0.32656332926792686 1101 21 0.34672870130036415 1101 22 0.05354497303120809 1101 23 -0.14126981286696605 1101 24 -0.05485863563726667 1101 25 0.10073392902916822 1102 1 0.5940706487846263 1102 2 0.5186092106114728 1102 3 0.6036831874258404 1102 4 0.528553396768416 1102 5 0.5464268607795191 1102 6 0.6746336458860895 1102 7 0.7290336676367772 1102 8 0.6076181664617621 1102 9 0.686128750086489 1102 10 0.8239758741567869 1102 11 0.8352118202281176 1102 12 0.9528312649325557 1102 13 1.0839443080565232 1102 14 1.1477805383352404 1102 15 1.1877658891743508 1102 16 1.3072806885614838 1102 17 1.364505258384625 1102 18 1.349146184954634 1102 19 1.3960972037330706 1102 20 1.3546676614402742 1102 21 1.4076129418983427 1102 22 1.1469459435630398 1102 23 1.2718891117211442 1102 24 1.137496469438453 1102 25 1.2541660113678361 1103 1 0.4257704998527281 1103 2 0.32804459576906453 1103 3 0.25527918407582106 1103 4 0.3014635184529695 1103 5 0.4789300156345817 1103 6 0.6008545653226491 1103 7 0.6592971596160524 1103 8 0.42142392591969724 1103 9 0.5603405100219092 1103 10 0.5948173393738045 1103 11 0.5890450165036825 1103 12 0.5713169155527282 1103 13 0.5296140089421542 1103 14 0.7757913880049756 1103 15 0.7840102852801394 1103 16 0.5203139348599777 1103 17 0.5293848054497587 1103 18 0.849631729465564 1103 19 0.8597225774839146 1103 20 0.9533956769322174 1103 21 0.7318150383137407 1103 22 0.8429595520730047 1103 23 0.8152764218140263 1103 24 0.954676881672853 1103 25 0.679361839758883 1104 1 0.5101902387172323 1104 2 0.5039097196516487 1104 3 0.5310437702945592 1104 4 0.5170720133878532 1104 5 0.5686920056106786 1104 6 0.5320618272006428 1104 7 0.5080553927774645 1104 8 0.6676654144924683 1104 9 0.7512960114727392 1104 10 0.6947887436026292 1104 11 0.8243814262955667 1104 12 0.7985763773821262 1104 13 0.7673720099891597 1104 14 0.9451127406754409 1104 15 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-0.8302716827217509 2315 6 -0.9027052791279004 2315 7 -0.9555904969135938 2315 8 -1.0460670885108598 2315 9 -1.07231090532904 2315 10 -1.0693761879919066 2315 11 -1.1421180508347106 2315 12 -1.091916218885359 2315 13 -1.1451557217769874 2315 14 -1.1898778501505092 2315 15 -1.368226305132346 2315 16 -1.3974937122079538 2315 17 -1.317857273693825 2315 18 -1.4117031572980778 2315 19 -1.463924258583944 2315 20 -1.4032641934768435 2315 21 -1.4909826195876335 2315 22 -1.6144974714685958 2315 23 -1.4070184474375225 2315 24 -1.3224995379175084 2315 25 -1.4132144579236794 2316 1 -1.4529941393099506 2316 2 -1.1421711520644813 2316 3 -0.9210078329023084 2316 4 -0.8577023455502157 2316 5 -0.8708548779254244 2316 6 -0.9318832304141509 2316 7 -0.9790339196244346 2316 8 -1.0168965002900023 2316 9 -1.2271086715193746 2316 10 -1.2748214491591128 2316 11 -1.0675661475039675 2316 12 -1.124571125148485 2316 13 -1.2243065091268974 2316 14 -1.3121252367315108 2316 15 -1.2771987489323067 2316 16 -1.2964108074122436 2316 17 -1.4382898812165976 2316 18 -1.4233085033455524 2316 19 -1.3337604217165548 2316 20 -1.3459548568067419 2316 21 -1.5225083781169175 2316 22 -1.4269134188287396 2316 23 -1.366494878626142 2316 24 -1.3179467091505046 2316 25 -1.3129976517413624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32726.0 3 0.0 4 575.0 5 5206.0 6 6858.0 7 5370.0 8 4479.0 9 4074.0 10 3740.0 11 3596.0 12 3461.0 13 3309.0 14 3177.0 15 3354.0 16 3361.0 17 3551.0 18 3961.0 19 4310.0 20 5289.0 21 6912.0 22 8833.0 23 11280.0 24 13374.0 25 16240.0 26 18634.0 27 21284.0 28 25508.0 29 30879.0 30 39046.0 31 50371.0 32 65071.0 33 85692.0 34 112041.0 35 151338.0 36 224392.0 37 406574.0 38 861846.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.566177149841955 20.14293230797655 13.963702833967265 24.327187708214236 2 14.671255698507188 21.017312438214315 41.47735354569829 22.834078317580207 3 18.69901569623134 25.671552625402715 30.378599571856306 25.250832106509634 4 12.654864267070629 17.845706472650722 38.712510756932446 30.786918503346207 5 12.81700604989754 38.3737437914855 35.93168733318542 12.877562825431536 6 30.733089390997602 38.15848428598861 18.30309835214463 12.80532797086916 7 27.24179806126295 32.02316563186754 22.730776206021037 18.004260100848466 8 24.425970968728443 37.87582588349087 20.72740866386453 16.970794483916162 9 26.038399581813138 15.391258970037052 20.799773482116823 37.770567966032985 10 14.933555939604714 29.33317686886144 34.69693009594117 21.036337095592682 11 33.49899898298093 22.553731322053665 25.536246417319198 18.411023277646205 12 23.331430867595497 24.867671950898604 32.23230351262739 19.568593668878506 13 28.762703848314807 22.408290483404098 26.2175336220814 22.61147204619969 14 20.521115591684623 22.919689275782858 28.095196185111693 28.463998947420826 15 22.398244753106177 31.99298399083972 23.764419623489587 21.844351632564525 16 21.411461294361807 28.64430142360508 28.664837394669807 21.279399887363308 17 21.03473689076646 29.296861109333104 28.406658274481355 21.261743725419077 18 20.8786817506782 27.654129797116163 30.958643132384562 20.508545319821078 19 23.179544759506996 26.985276337593433 28.36243039109006 21.47274851180951 20 23.16523181633916 27.562639128919614 28.902855121011044 20.369273933730184 21 22.436738569203523 26.912555918268648 27.94664383707781 22.704061675450014 22 22.796162353225657 28.401546509064275 28.144269133115706 20.658022004594365 23 21.738649213766028 28.214366994530852 28.682115755261115 21.364868036441997 24 22.752236972129477 26.99542714843342 29.36649091541936 20.885844964017743 25 22.20691653898349 27.713939891781557 28.957033998619373 21.122109570615578 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 235.0 1 235.0 2 474.0 3 713.0 4 713.0 5 713.0 6 1784.5 7 2856.0 8 2856.0 9 2856.0 10 4091.5 11 5327.0 12 5327.0 13 5327.0 14 8544.0 15 11761.0 16 11761.0 17 11761.0 18 21079.5 19 30398.0 20 30398.0 21 30398.0 22 47684.5 23 64971.0 24 64971.0 25 64971.0 26 93617.5 27 122264.0 28 122264.0 29 122264.0 30 156644.5 31 191025.0 32 191025.0 33 191025.0 34 212317.0 35 233609.0 36 233609.0 37 233609.0 38 247178.5 39 260748.0 40 260748.0 41 260748.0 42 270906.5 43 281065.0 44 281065.0 45 281065.0 46 284808.5 47 288552.0 48 288552.0 49 288552.0 50 274947.5 51 261343.0 52 261343.0 53 261343.0 54 228832.5 55 196322.0 56 196322.0 57 196322.0 58 166879.5 59 137437.0 60 137437.0 61 137437.0 62 110957.5 63 84478.0 64 84478.0 65 84478.0 66 64505.5 67 44533.0 68 44533.0 69 44533.0 70 32460.5 71 20388.0 72 20388.0 73 20388.0 74 14148.5 75 7909.0 76 7909.0 77 7909.0 78 5314.0 79 2719.0 80 2719.0 81 2719.0 82 1752.0 83 785.0 84 785.0 85 785.0 86 497.0 87 209.0 88 209.0 89 209.0 90 132.5 91 56.0 92 56.0 93 56.0 94 30.5 95 5.0 96 5.0 97 5.0 98 4.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39698414730418824 2 0.0 3 0.0 4 0.0 5 0.025914428157915323 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 4.445013406160433E-5 17 0.0 18 4.445013406160433E-5 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005822967562070167 25 1.33350402184813E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2249712.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.06052620377012 #Duplication Level Percentage of deduplicated Percentage of total 1 82.44116733356294 56.934303969089214 2 11.923589272703161 16.468986988210183 3 3.013515549950342 6.243449088084431 4 1.052628223879535 2.907802361522425 5 0.5135450672923978 1.773284628828177 6 0.2802631310989444 1.1613071585525587 7 0.15690191109159324 0.7585009979653808 8 0.11889473778586498 0.6568746523480886 9 0.08391016529528367 0.5215392152223859 >10 0.3689177002255326 4.5101839741909515 >50 0.025454514304796092 1.2334292131817468 >100 0.01710209807594624 2.459129113509913 >500 0.0021193707220337385 1.0834547678017667 >1k 0.0017340305907543802 2.2035894558983142 >5k 2.568934208525008E-4 1.0841644155944365 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8508 0.37818174059612963 No Hit GGTATCAACGCAGAGTACTTTTTTT 5502 0.24456463760694705 No Hit TATCAACGCAGAGTACTTTTTTTTT 5432 0.2414531282226347 No Hit GTCCTACAGTGGACATTTCTAAATT 5002 0.22233957057614484 No Hit CTGTAGGACGTGGAATATGGCAAGA 4827 0.21456079711536408 No Hit CTTTAGGACGTGAAATATGGCGAGG 4278 0.1901576735155433 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4257 0.18922422070024963 No Hit GTCCTACAGTGTGCATTTCTCATTT 2661 0.11828180673792911 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2613 0.11614820030297211 No Hit CTGAAGGACCTGGAATATGGCGAGA 2269 0.10085735418578021 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.445013406160433E-5 2 0.0 0.0 0.0 0.0 4.445013406160433E-5 3 0.0 0.0 0.0 0.0 4.445013406160433E-5 4 0.0 0.0 0.0 0.0 4.445013406160433E-5 5 0.0 0.0 0.0 0.0 4.445013406160433E-5 6 0.0 0.0 0.0 0.0 4.445013406160433E-5 7 0.0 0.0 0.0 0.0 4.445013406160433E-5 8 0.0 0.0 0.0 0.0 4.445013406160433E-5 9 0.0 0.0 0.0 0.0 4.445013406160433E-5 10 0.0 0.0 0.0 0.0 4.445013406160433E-5 11 0.0 0.0 0.0 0.0 4.445013406160433E-5 12 0.0 0.0 0.0 0.0 8.890026812320866E-5 13 0.0 0.0 0.0 0.0 8.890026812320866E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1690 0.0 13.258569 1 GGCGCCC 55 0.0030745093 12.088108 18 TAGGACC 835 0.0 11.604681 4 TCCAACG 165 7.2759576E-12 11.512484 18 ACTGCGT 75 2.079929E-4 11.3968525 8 GTATTAC 170 1.0913936E-11 11.217513 1 GCGCCCA 60 0.005887186 11.080767 19 GTCTAGA 235 0.0 10.954976 1 GTGGTAT 505 0.0 10.950958 1 AATCCCG 220 0.0 10.7929535 19 GGCGAGG 1230 0.0 10.733286 19 GGACCGT 90 9.545176E-5 10.552642 6 TTAGGAC 1690 0.0 10.511736 3 TGGTATC 525 0.0 10.314189 2 CCTACAC 410 0.0 10.195026 3 TAGGACA 855 0.0 9.999907 4 TGTAGGA 2945 0.0 9.9676485 2 TGGCGAG 2765 0.0 9.961526 18 CCCGTTT 115 1.0298958E-5 9.910527 12 CCACCTT 840 0.0 9.836788 13 >>END_MODULE