FastQCFastQC Report
Thu 26 May 2016
SRR1511786_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511786_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956244
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57190.29234594457542107No Hit
GTCCTACAGTGGACATTTCTAAATT54290.2775216179576781No Hit
CTGTAGGACGTGGAATATGGCAAGA51490.263208475016409No Hit
GTCCTAAAGTGTGTATTTCTCATTT46560.23800711976624594No Hit
CTTTAGGACGTGAAATATGGCGAGG41970.2145437890160941No Hit
GGTATCAACGCAGAGTACTTTTTTT36090.18448618883942905No Hit
TATCAACGCAGAGTACTTTTTTTTT32340.16531680097165793No Hit
GTCCTACAGTGTGCATTTCTCATTT27840.1423135355303326No Hit
CTGAAGGACCTGGAATATGGCGAGA22600.11552751088310047No Hit
CTGTAGGACCTGGAATATGGCGAGA20140.10295239244184265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG1004.956746E-913.9893441
GGTATCA11200.013.471881
AATAGCG500.001566777213.2230827
CCAACGA957.96972E-812.92514819
TCCAACG1459.094947E-1212.37660118
TAGGACC8700.011.7252014
GGCGAGG13000.010.9710819
GACGTGG15700.010.768577
AGGACGT30900.010.6985615
GGACGTG30350.010.6434696
GAAATGT8650.010.591846
AGGACCT17050.010.5255565
ATCCCGT1554.0290615E-910.359097510
GTAGAAC6400.010.3307973
AATGTCC7550.010.258218
GTAGGAC31300.010.2298953
TGTAGGA30500.010.2217212
GGACCTG16550.010.2157646
TAGAAAT8550.010.1633644
TTCCACC7350.010.15209211
Produced by FastQC (version 0.11.5)