FastQCFastQC Report
Thu 26 May 2016
SRR1511786_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511786_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956244
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71700.36651869603178333No Hit
GTCCTACAGTGGACATTTCTAAATT55920.28585391188420256No Hit
CTGTAGGACGTGGAATATGGCAAGA55340.282889046560654No Hit
TATCAACGCAGAGTACTTTTTTTTT48410.247464017781013No Hit
GGTATCAACGCAGAGTACTTTTTTT48320.24700395247218648No Hit
GTCCTAAAGTGTGTATTTCTCATTT47250.24153428713391578No Hit
CTTTAGGACGTGAAATATGGCGAGG44900.22952147073677925No Hit
GTCCTACAGTGTGCATTTCTCATTT28650.14645412330977117No Hit
CTGAAGGACCTGGAATATGGCGAGA26210.13398124160380812No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23030.11772560069193821No Hit
CTGTAGGACCTGGAATATGGCGAGA22730.11619204966251653No Hit
TTTCTAAATTTTCCACCTTTTTCAG20280.10366804958890609No Hit
ATTTAGAAATGTCCACTGTAGGACG19990.1021856169271318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGT707.2850617E-613.56777819
GGTATCA12650.013.4904481
TCCCCCG604.1012853E-412.66358217
AATACGG853.95554E-612.29112414
TGTCGAG1551.8189894E-1212.25507918
CCCCCGG701.0936619E-412.21131118
GGCGAGG10750.012.01537119
AATCCCG1900.011.99677119
TAGGACC10100.011.945694
TATGTCG1701.8189894E-1211.73243716
GAATACG658.0414617E-411.6894613
GTCGAGG1406.8394E-1011.53261119
ATGTCGA1701.2732926E-1111.17374917
CTAGGAC3000.011.08346753
ATACGGC803.7801114E-410.68489715
CACCTTT8100.010.67024114
GTGGTAT3250.010.2671181
AGGACCT18900.010.2038275
GACGTGA15300.010.1802677
AATGTCC7400.010.1391648
Produced by FastQC (version 0.11.5)