##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511786_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1956244 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.538516156471278 31.0 31.0 34.0 27.0 34.0 2 30.742784131222894 31.0 31.0 34.0 27.0 34.0 3 30.854064216938173 31.0 31.0 34.0 27.0 34.0 4 34.45615679843619 37.0 35.0 37.0 31.0 37.0 5 34.06339444363791 37.0 35.0 37.0 30.0 37.0 6 33.997914881783664 37.0 35.0 37.0 30.0 37.0 7 33.81683470978058 37.0 35.0 37.0 29.0 37.0 8 33.71632015229184 37.0 35.0 37.0 29.0 37.0 9 35.164966640153274 39.0 35.0 39.0 29.0 39.0 10 34.946931977810536 38.0 35.0 39.0 28.0 39.0 11 34.934286316021925 38.0 35.0 39.0 28.0 39.0 12 34.74898376685117 38.0 35.0 39.0 27.0 39.0 13 34.73092364756135 38.0 35.0 39.0 27.0 39.0 14 35.67389446306289 39.0 36.0 41.0 27.0 41.0 15 35.61230040833352 39.0 36.0 41.0 27.0 41.0 16 35.499724471998384 39.0 35.0 41.0 27.0 41.0 17 35.43703597301768 39.0 35.0 41.0 27.0 41.0 18 35.36066053109939 39.0 35.0 40.0 27.0 41.0 19 35.375701088412285 39.0 35.0 40.0 27.0 41.0 20 35.232562502428124 39.0 35.0 40.0 26.0 41.0 21 35.10859381549541 39.0 35.0 40.0 26.0 41.0 22 35.19652558678774 39.0 35.0 40.0 26.0 41.0 23 35.07822797156183 39.0 35.0 40.0 26.0 41.0 24 34.94913057880305 39.0 35.0 41.0 25.0 41.0 25 34.47861974273148 39.0 34.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4535411495684478 1101 2 0.44006438889982036 1101 3 0.5092211128178086 1101 4 0.4854820962977726 1101 5 0.5128225646874256 1101 6 0.45923959238908907 1101 7 0.3673407838631064 1101 8 0.5127777368244963 1101 9 0.3666233693420651 1101 10 0.3266056332706029 1101 11 0.16893718174202377 1101 12 0.3399469405902096 1101 13 0.2126154883841238 1101 14 0.2586580685675912 1101 15 0.11182983523236345 1101 16 0.2583774100284657 1101 17 0.242156339287682 1101 18 0.4210563742213509 1101 19 0.3007656028774406 1101 20 0.07449032995647542 1101 21 0.3048266726745297 1101 22 0.22463234538289356 1101 23 0.2252057546427011 1101 24 0.19915759107403375 1101 25 0.2153569137831184 1102 1 0.5499994880239356 1102 2 0.5498854003816405 1102 3 0.5831989359437166 1102 4 0.5621545817303684 1102 5 0.5756799885312489 1102 6 0.682966634898861 1102 7 0.7724928503445554 1102 8 0.6002030558321962 1102 9 0.5938293782423187 1102 10 0.7427932761786735 1102 11 0.8959403533792312 1102 12 0.809616008522255 1102 13 0.9185850505014983 1102 14 1.1390012938030054 1102 15 1.1505680645245278 1102 16 1.0394582253113356 1102 17 1.2559197695731257 1102 18 1.2491855999976593 1102 19 1.2274454642105113 1102 20 1.3578527571194599 1102 21 1.4586781220389682 1102 22 1.1743028884950277 1102 23 1.3400767993160727 1102 24 1.4617076857314188 1102 25 1.6041148121432087 1103 1 0.5727938502878374 1103 2 0.591677602575448 1103 3 0.5314861081261348 1103 4 0.6139376938244183 1103 5 0.7058933247384331 1103 6 0.718313768940348 1103 7 0.7067231672329299 1103 8 0.7000500703904109 1103 9 0.8699833289214993 1103 10 0.8047195807719802 1103 11 0.7069974757535249 1103 12 0.8164909821656465 1103 13 0.6124759411907092 1103 14 0.8369897578104286 1103 15 0.9666610553079096 1103 16 0.6620152610016916 1103 17 0.8187761694250639 1103 18 0.8398663734995537 1103 19 0.9842665532419446 1103 20 0.9754406944633658 1103 21 0.8702839303315812 1103 22 1.0029135089177643 1103 23 1.059683535361124 1103 24 1.1047033890193205 1103 25 0.9628715301486395 1104 1 0.4531588436883176 1104 2 0.44203053342620535 1104 3 0.5617201238412086 1104 4 0.5651079974473419 1104 5 0.5388025371732112 1104 6 0.6213157347308709 1104 7 0.7280323589633326 1104 8 0.7977013051701647 1104 9 0.7586897104828978 1104 10 0.8988741389417143 1104 11 0.9019716571711243 1104 12 0.9082521594344968 1104 13 0.9873463981835542 1104 14 1.0617309812365292 1104 15 1.0727912385753982 1104 16 1.1859406307267122 1104 17 1.208904289006341 1104 18 1.1399419894828782 1104 19 1.1705699331188697 1104 20 1.2346797411168566 1104 21 1.2731286441329601 1104 22 1.4238213845699832 1104 23 1.3888406421945874 1104 24 1.4241840129862098 1104 25 1.4202185456240315 1105 1 0.27099226874035054 1105 2 0.26647625595576585 1105 3 0.3948502968102616 1105 4 0.30732880366159776 1105 5 0.3733094560731942 1105 6 0.5035596844000594 1105 7 0.522702192338727 1105 8 0.6611557485617965 1105 9 0.6815574576372043 1105 10 0.611326503835933 1105 11 0.6128591493612419 1105 12 0.6376982750441869 1105 13 0.6490114304643981 1105 14 0.7690520642619489 1105 15 0.7969532537938093 1105 16 0.6733704797149827 1105 17 0.5457099681177837 1105 18 0.727809743533328 1105 19 0.7709903219785659 1105 20 0.8509154678254802 1105 21 0.9456299992249981 1105 22 0.8624792296908907 1105 23 0.9830473306254746 1105 24 1.103517790431134 1105 25 0.9840377949651042 1106 1 0.22166324863761488 1106 2 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14 0.6562863212701444 1107 15 0.7979402271963281 1107 16 0.8596971888763392 1107 17 1.0396111336978962 1107 18 0.8343617471135261 1107 19 0.8500481816815864 1107 20 0.8697163435224695 1107 21 0.9858152986432174 1107 22 0.7807491233735391 1107 23 0.8932408145045088 1107 24 0.6460330405409991 1107 25 0.7084375675741725 1108 1 0.2687041265403032 1108 2 0.23945252996112032 1108 3 0.1602890847835461 1108 4 0.3116140353343724 1108 5 0.2853556606936536 1108 6 0.4601629431511327 1108 7 0.3357021143781793 1108 8 0.3321180416414933 1108 9 0.4426941224847951 1108 10 0.4483655620724676 1108 11 0.4926049979618483 1108 12 0.589340644005496 1108 13 0.6619035070736317 1108 14 0.7474388005733772 1108 15 0.6702790509404508 1108 16 0.7163371385855939 1108 17 0.727699781272328 1108 18 0.7062549502587245 1108 19 0.7516833912854963 1108 20 0.5417836885058165 1108 21 0.7083921415809726 1108 22 0.8087851988704884 1108 23 0.8033846399469624 1108 24 0.9707951480881576 1108 25 0.7580535429965991 1109 1 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22 -0.6945811377631728 2313 23 -0.5665613546524995 2313 24 -0.641756607087359 2313 25 -0.5692191842761218 2314 1 -0.20607341450494232 2314 2 -0.27206251846958196 2314 3 -0.39218795315532873 2314 4 -0.2566446078898039 2314 5 -0.5558346066151714 2314 6 -0.3008158762497928 2314 7 -0.4967813690515399 2314 8 -0.4346357585124778 2314 9 -0.5547178339007175 2314 10 -0.6760530009411596 2314 11 -0.5816703319219556 2314 12 -0.5954077132895463 2314 13 -0.5881931752889997 2314 14 -0.7316643809925196 2314 15 -0.6393811284590427 2314 16 -0.6343253146398382 2314 17 -0.6111727949545553 2314 18 -0.6945032901222703 2314 19 -0.6238559709689966 2314 20 -0.5498864097879235 2314 21 -0.47810097427672815 2314 22 -0.5778180274557627 2314 23 -0.801983021593152 2314 24 -0.987788992423539 2314 25 -0.7747674334952634 2315 1 -0.6357190185381363 2315 2 -0.6615021282595741 2315 3 -0.8278707756066623 2315 4 -0.729202538393686 2315 5 -0.8803984430357019 2315 6 -0.9715442262064897 2315 7 -0.898339660891331 2315 8 -1.0038987725455897 2315 9 -0.9433561137123689 2315 10 -1.0740724950544944 2315 11 -1.1036548772327421 2315 12 -0.9569869724444118 2315 13 -1.1835975314760745 2315 14 -1.3674068525465373 2315 15 -1.436337385510626 2315 16 -1.36540188262056 2315 17 -1.4610311693034745 2315 18 -1.388382948057611 2315 19 -1.49335041204953 2315 20 -1.5241707229752066 2315 21 -1.649620617103146 2315 22 -1.7371366429738657 2315 23 -1.5796737655764161 2315 24 -1.3302842258211385 2315 25 -1.475793307932264 2316 1 -1.5050246562416874 2316 2 -1.3314196741781394 2316 3 -1.199534277195017 2316 4 -1.209143088521067 2316 5 -1.310688709905719 2316 6 -1.0922162906457018 2316 7 -1.255645527951394 2316 8 -1.3369069764036396 2316 9 -1.4899278467272623 2316 10 -1.552834269052319 2316 11 -1.4554545260098024 2316 12 -1.57194766501798 2316 13 -1.5263664753578965 2316 14 -1.8717462403516265 2316 15 -1.7665114575464358 2316 16 -1.7301589278023357 2316 17 -1.8335258804960475 2316 18 -1.9598095310174628 2316 19 -1.852668550098464 2316 20 -1.8225695693784374 2316 21 -2.2967950965376147 2316 22 -2.0789736182766987 2316 23 -2.00778473008495 2316 24 -1.8274493668722798 2316 25 -1.792748841230818 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37612.0 3 0.0 4 673.0 5 5906.0 6 7348.0 7 5853.0 8 4699.0 9 4244.0 10 3988.0 11 3848.0 12 3513.0 13 3470.0 14 3367.0 15 3424.0 16 3428.0 17 3537.0 18 3953.0 19 4418.0 20 5344.0 21 6790.0 22 8908.0 23 10951.0 24 13531.0 25 15700.0 26 18250.0 27 20627.0 28 24252.0 29 29509.0 30 37361.0 31 47952.0 32 61755.0 33 80396.0 34 105147.0 35 141018.0 36 209251.0 37 369471.0 38 646750.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.13333689183058 20.785695662260288 13.678179089585852 24.402788356323278 2 14.55196795491769 21.768603507537915 41.67271567350494 22.006712864039454 3 18.632900599311743 26.132578553595565 30.760988915493158 24.473531931599535 4 12.448549362962902 18.27164709514764 39.35674690887231 29.923056633017147 5 12.21112645088715 38.83044434217927 36.772613386511225 12.185815820422356 6 30.24740267573984 39.09609435223827 18.36780074469238 12.288702227329514 7 26.74502771637894 32.25400307937047 23.367279337342374 17.633689866908217 8 23.796162441903977 39.05366610709093 21.047783405342074 16.102388045663016 9 25.993689948697607 15.133490505274395 21.110658997548363 37.76216054847964 10 14.32147523519561 29.85583597956083 35.70127243840748 20.121416346836078 11 33.35626844095113 22.494075381189667 26.196016447845977 17.95363973001323 12 23.04329112319322 25.220780229869078 33.05789052899332 18.678038117944386 13 28.81184555709819 22.871686763000934 26.36117989371469 21.95528778618618 14 19.710271315848125 23.591177787637942 28.677966552229684 28.02058434428425 15 21.754085891126056 33.298197975303694 23.79861612355105 21.1491000100192 16 20.569489868840357 29.097729217550793 29.726894729793145 20.605886183815702 17 20.46912348357362 29.95336982503205 29.053328725864464 20.524177965529862 18 20.24114591080965 27.987934014332577 32.09764840053102 19.67327167432676 19 22.82317543210356 27.358090299574084 28.85033768793668 20.96839658038568 20 22.823891089250626 28.146591120535064 29.59697256579445 19.432545224419858 21 21.7247950664641 27.436096928603998 28.46137802850769 22.37772997642421 22 22.234803020482108 29.414582230028564 28.657416968435427 19.693197781053897 23 20.760958244472572 29.140587779438558 29.45266541392587 20.645788562163002 24 22.29546109819462 27.457421596232614 30.21890892137437 20.028208384198397 25 21.535270176184746 28.507567059699156 29.613667429694278 20.343495334421817 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 246.0 1 246.0 2 443.0 3 640.0 4 640.0 5 640.0 6 1724.0 7 2808.0 8 2808.0 9 2808.0 10 4249.5 11 5691.0 12 5691.0 13 5691.0 14 8688.0 15 11685.0 16 11685.0 17 11685.0 18 20425.5 19 29166.0 20 29166.0 21 29166.0 22 46282.5 23 63399.0 24 63399.0 25 63399.0 26 90788.5 27 118178.0 28 118178.0 29 118178.0 30 150521.0 31 182864.0 32 182864.0 33 182864.0 34 202419.5 35 221975.0 36 221975.0 37 221975.0 38 231338.5 39 240702.0 40 240702.0 41 240702.0 42 245647.5 43 250593.0 44 250593.0 45 250593.0 46 249457.5 47 248322.0 48 248322.0 49 248322.0 50 231764.0 51 215206.0 52 215206.0 53 215206.0 54 183279.0 55 151352.0 56 151352.0 57 151352.0 58 126442.5 59 101533.0 60 101533.0 61 101533.0 62 80909.5 63 60286.0 64 60286.0 65 60286.0 66 45471.0 67 30656.0 68 30656.0 69 30656.0 70 22030.0 71 13404.0 72 13404.0 73 13404.0 74 9226.5 75 5049.0 76 5049.0 77 5049.0 78 3401.0 79 1753.0 80 1753.0 81 1753.0 82 1148.0 83 543.0 84 543.0 85 543.0 86 347.5 87 152.0 88 152.0 89 152.0 90 92.5 91 33.0 92 33.0 93 33.0 94 20.5 95 8.0 96 8.0 97 8.0 98 4.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40158589623789265 2 0.0 3 0.0 4 0.0 5 0.02780839200017994 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0223673529477918E-4 17 0.0 18 5.111836764738959E-5 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004498416352970284 25 1.0223673529477918E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1956244.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.77969165806047 #Duplication Level Percentage of deduplicated Percentage of total 1 86.77934506055922 64.02553320856494 2 9.738471644646985 14.370028703256391 3 1.898037944844818 4.201099629778485 4 0.5992720650766229 1.7685643272256957 5 0.2765745812984425 1.0202793664328131 6 0.16124512605349528 0.7137969408955185 7 0.10166153218200591 0.5250389548512072 8 0.0695739022254124 0.41065128429111797 9 0.051133042528400195 0.3395322100155474 >10 0.28113740309800106 3.782204838937299 >50 0.021572952793345677 1.137522820378588 >100 0.01755373993264697 2.666770685722899 >500 0.002210502380304908 1.1592144486636824 >1k 0.0020032677821513025 2.947294453803983 >5k 2.0723459815358305E-4 0.9324681271818236 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7170 0.36651869603178333 No Hit GTCCTACAGTGGACATTTCTAAATT 5592 0.28585391188420256 No Hit CTGTAGGACGTGGAATATGGCAAGA 5534 0.282889046560654 No Hit TATCAACGCAGAGTACTTTTTTTTT 4841 0.247464017781013 No Hit GGTATCAACGCAGAGTACTTTTTTT 4832 0.24700395247218648 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4725 0.24153428713391578 No Hit CTTTAGGACGTGAAATATGGCGAGG 4490 0.22952147073677925 No Hit GTCCTACAGTGTGCATTTCTCATTT 2865 0.14645412330977117 No Hit CTGAAGGACCTGGAATATGGCGAGA 2621 0.13398124160380812 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2303 0.11772560069193821 No Hit CTGTAGGACCTGGAATATGGCGAGA 2273 0.11619204966251653 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2028 0.10366804958890609 No Hit ATTTAGAAATGTCCACTGTAGGACG 1999 0.1021856169271318 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0223673529477918E-4 0.0 10 0.0 0.0 0.0 2.5559183823694795E-4 0.0 11 0.0 0.0 0.0 2.5559183823694795E-4 0.0 12 0.0 0.0 0.0 2.5559183823694795E-4 0.0 13 0.0 0.0 0.0 2.5559183823694795E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCCGGT 70 7.2850617E-6 13.567778 19 GGTATCA 1265 0.0 13.490448 1 TCCCCCG 60 4.1012853E-4 12.663582 17 AATACGG 85 3.95554E-6 12.291124 14 TGTCGAG 155 1.8189894E-12 12.255079 18 CCCCCGG 70 1.0936619E-4 12.211311 18 GGCGAGG 1075 0.0 12.015371 19 AATCCCG 190 0.0 11.996771 19 TAGGACC 1010 0.0 11.94569 4 TATGTCG 170 1.8189894E-12 11.732437 16 GAATACG 65 8.0414617E-4 11.68946 13 GTCGAGG 140 6.8394E-10 11.532611 19 ATGTCGA 170 1.2732926E-11 11.173749 17 CTAGGAC 300 0.0 11.0834675 3 ATACGGC 80 3.7801114E-4 10.684897 15 CACCTTT 810 0.0 10.670241 14 GTGGTAT 325 0.0 10.267118 1 AGGACCT 1890 0.0 10.203827 5 GACGTGA 1530 0.0 10.180267 7 AATGTCC 740 0.0 10.139164 8 >>END_MODULE