FastQCFastQC Report
Thu 26 May 2016
SRR1511785_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511785_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022435
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT51310.25370407454380484No Hit
CTGTAGGACGTGGAATATGGCAAGA47280.2337775997745292No Hit
GTATCAACGCAGAGTACTTTTTTTT46760.23120644173978397No Hit
GTCCTAAAGTGTGTATTTCTCATTT43710.21612561095906668No Hit
CTTTAGGACGTGAAATATGGCGAGG39980.19768249659445175No Hit
GTCCTACAGTGTGCATTTCTCATTT28920.14299594300929325No Hit
GGTATCAACGCAGAGTACTTTTTTT27710.13701305604382835No Hit
TATCAACGCAGAGTACTTTTTTTTT26830.13266186552349024No Hit
CTGTAGGACCTGGAATATGGCGAGA23080.11411986046523127No Hit
CTGAAGGACCTGGAATATGGCGAGA21860.10808752815294434No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC509.157887E-515.1121048
CGGTAGG755.464499E-714.6564621
GCGTACT450.00823776613.3240571
GGATCGT500.001566801813.2230916
TAGGACC9450.013.093164
GGTATCA10900.012.926781
TACACCG803.0426305E-511.8063325
GTATCAA22400.011.0637251
AGGATCG600.006135856311.0192425
GGCGAGG12750.010.8160619
AAAGGCG1053.7166847E-610.7943615
GGACCTG17650.010.5955986
AGGACCT19150.010.5547985
TGTAGGA29350.010.4186392
TCCAACG1002.5564817E-510.39008518
CCTATAC2100.010.3445963
GTCCTAC28350.010.2574081
GTCCTAA15650.010.1526761
ATAGGAC2700.010.1446993
CTGTAGG29650.010.0435971
Produced by FastQC (version 0.11.5)