FastQCFastQC Report
Thu 26 May 2016
SRR1511785_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511785_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022435
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56250.2781300758738847No Hit
GTCCTACAGTGGACATTTCTAAATT51100.25266572226054235No Hit
CTGTAGGACGTGGAATATGGCAAGA51060.2524679408732543No Hit
GTCCTAAAGTGTGTATTTCTCATTT45490.22492688269338695No Hit
CTTTAGGACGTGAAATATGGCGAGG44160.21835065156605776No Hit
TATCAACGCAGAGTACTTTTTTTTT35220.17414651150716834No Hit
GGTATCAACGCAGAGTACTTTTTTT33560.16593858393471236No Hit
GTCCTACAGTGTGCATTTCTCATTT30610.1513522066222153No Hit
CTGTAGGACCTGGAATATGGCGAGA24700.12213000665039916No Hit
CTGAAGGACCTGGAATATGGCGAGA24020.11876772306650152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10400.013.3864051
TAGGACC10250.012.2346414
GAACCGT752.079826E-411.3967966
TACACCG855.322279E-511.1769215
GGCGAGG13400.011.05686719
GTATTAG1657.4578566E-1110.9802971
GTATCAA22500.010.6797841
TGGCGAG28550.010.5121918
GTCTAGC1105.7835277E-610.4023871
GATATAC5050.010.38522151
CTAGGAC2900.010.1555823
CGCTGCC750.002654342410.1304867
TTAGGAC18000.010.0809623
AGGACGT28400.010.0021645
GTCCTAA15150.09.8816951
TGTAGGA29750.09.8676252
GGACGTG28250.09.8167096
AGGACCT19800.09.7882745
TCTAGGA4250.09.6121532
GTAGGAC30350.09.6099433
Produced by FastQC (version 0.11.5)