FastQCFastQC Report
Thu 26 May 2016
SRR1511784_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511784_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101999
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64900.3087537149161346No Hit
GGTATCAACGCAGAGTACTTTTTTT43000.20456717629266236No Hit
TATCAACGCAGAGTACTTTTTTTTT39900.18981931009481925No Hit
GTCCTACAGTGGACATTTCTAAATT28650.13629882792522738No Hit
CTGTAGGACGTGGAATATGGCAAGA27570.13116086163694654No Hit
GTCCTAAAGTGTGTATTTCTCATTT24950.1186965360116727No Hit
CTTTAGGACGTGAAATATGGCGAGG23250.11060899648382325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14150.014.1971881
TAGGACC5550.012.0829164
AGCCGTT550.003205757412.0207477
TCCAACG1355.178663E-911.19445518
GGCGAGG8750.011.01054719
GTGGTAT4250.010.8176171
AGATCGG803.9801942E-410.62548210
GCGCCAC803.9801942E-410.62548213
GTCCTAA9700.010.6127571
TTAGGAC11950.010.5911553
TGGCGAG18100.010.48900418
GTTTAGG2300.010.4290821
AGGACGT18700.010.4047595
GGACGTG18450.010.3407286
GTATCAA28750.010.3247921
GACGTGA9600.010.2319467
CGAGTAA750.00277918310.07453110
GTCTAGA2301.8189894E-129.9945371
GTCTTAA2500.09.9945371
TGGTATC4400.09.7673892
Produced by FastQC (version 0.11.5)