##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511784_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2101999 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.432119139923472 31.0 30.0 34.0 25.0 34.0 2 26.67130859719724 31.0 26.0 34.0 10.0 34.0 3 28.422590591146808 31.0 28.0 34.0 16.0 34.0 4 32.80731437074899 35.0 33.0 37.0 26.0 37.0 5 33.17712663041229 35.0 35.0 37.0 27.0 37.0 6 33.381340333653824 37.0 35.0 37.0 28.0 37.0 7 33.36259151407779 37.0 35.0 37.0 27.0 37.0 8 33.385702371885046 37.0 35.0 37.0 27.0 37.0 9 34.92542099211275 39.0 35.0 39.0 28.0 39.0 10 34.80182388288482 39.0 35.0 39.0 27.0 39.0 11 34.80210742250591 39.0 35.0 39.0 27.0 39.0 12 34.68227720374748 39.0 35.0 39.0 27.0 39.0 13 34.66764113589017 39.0 35.0 39.0 27.0 39.0 14 35.750097883015165 40.0 36.0 41.0 27.0 41.0 15 35.68109737445165 40.0 36.0 41.0 27.0 41.0 16 35.62962827289642 40.0 36.0 41.0 27.0 41.0 17 35.589456988323974 40.0 36.0 41.0 27.0 41.0 18 35.55579712454668 40.0 36.0 41.0 27.0 41.0 19 35.58297553899883 40.0 36.0 41.0 27.0 41.0 20 35.51630757198267 40.0 36.0 41.0 26.0 41.0 21 35.410206665179196 40.0 36.0 41.0 26.0 41.0 22 35.309645247214675 40.0 36.0 41.0 25.0 41.0 23 35.1942270191375 40.0 36.0 41.0 25.0 41.0 24 35.07401288012031 40.0 36.0 41.0 25.0 41.0 25 34.96544241933512 39.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8678223608831956 1101 2 3.4782276527903875 1101 3 2.07570602481546 1101 4 1.0786326440054452 1101 5 0.614714723165541 1101 6 0.5095030054527072 1101 7 0.3623145821124396 1101 8 0.48145067681978304 1101 9 0.5291455795104127 1101 10 0.41727592552734194 1101 11 0.5860050436694948 1101 12 0.4481943608173111 1101 13 0.3665348552149439 1101 14 0.2752534586428652 1101 15 0.4944498811295759 1101 16 0.6019499685544076 1101 17 0.40067751379684324 1101 18 0.6614700410330911 1101 19 0.6608863550193433 1101 20 0.4916706619281399 1101 21 0.618506152304839 1101 22 0.5112529727963491 1101 23 0.4148792719329393 1101 24 0.7271816471180514 1101 25 0.5913956757493537 1102 1 1.1897007127456263 1102 2 3.8967353944853826 1102 3 2.102034844734412 1102 4 1.2882190108215568 1102 5 0.808189310429853 1102 6 0.9507171032817752 1102 7 0.8464629722375321 1102 8 0.7905521646623797 1102 9 0.8000122271048014 1102 10 0.8086560370855267 1102 11 0.6504345927370281 1102 12 0.7218299048266914 1102 13 0.8473483298080637 1102 14 0.9859087611995534 1102 15 0.8449061956101502 1102 16 0.8608543745725399 1102 17 0.9891215878577952 1102 18 0.9820485693775325 1102 19 0.8884725982658352 1102 20 1.0027551785674333 1102 21 1.0636021515481957 1102 22 1.1442093407163867 1102 23 1.1396443505738745 1102 24 0.9727870928485913 1102 25 1.0822270737931419 1103 1 1.1674653765761285 1103 2 3.638361539522215 1103 3 2.2035272648884643 1103 4 1.44797342801796 1103 5 1.001399899085662 1103 6 0.7986831253870363 1103 7 0.9100762073564326 1103 8 0.7771771532733354 1103 9 0.7412548592702564 1103 10 0.9452054024530199 1103 11 1.0157095767923678 1103 12 0.9261074190016672 1103 13 1.0690639051124151 1103 14 1.1129277756443656 1103 15 0.8869919673667255 1103 16 1.0073260141040805 1103 17 0.9736501515768623 1103 18 1.0129830315694122 1103 19 1.0993704753482803 1103 20 1.1499173094748443 1103 21 1.1846649419687836 1103 22 1.2739121042237898 1103 23 1.3091730194225661 1103 24 1.37033827345428 1103 25 1.1912849020954397 1104 1 1.2368939076514778 1104 2 3.8832792525704285 1104 3 2.258164993808947 1104 4 1.39929506339967 1104 5 1.2559752150177914 1104 6 0.9786969799943606 1104 7 0.818408475812987 1104 8 0.9758745511628675 1104 9 1.0393785688077415 1104 10 1.0866703590396796 1104 11 1.0186378758366672 1104 12 0.9478373617673483 1104 13 1.1437160266832294 1104 14 1.228039385939823 1104 15 1.15891432776138 1104 16 1.1925241820676575 1104 17 1.1456824072548955 1104 18 1.1792751754690158 1104 19 1.2005612990248622 1104 20 1.1781277111912019 1104 21 1.2778087944911931 1104 22 1.253168932325778 1104 23 1.3539312027203678 1104 24 1.3458976700390508 1104 25 1.293567146003788 1105 1 1.2181781902108568 1105 2 3.9508859923993107 1105 3 2.347131569903347 1105 4 1.5562962387085335 1105 5 1.1454046097402468 1105 6 0.8989690359500244 1105 7 0.6626687030219784 1105 8 0.7181578853875692 1105 9 1.0061258069623662 1105 10 0.7107914536813951 1105 11 0.904649352382016 1105 12 0.7123065095836338 1105 13 0.8869643925343098 1105 14 0.9667339191230511 1105 15 1.1493701936744216 1105 16 1.087011970465099 1105 17 1.2446541098352242 1105 18 1.1324295296557096 1105 19 1.0666222663784097 1105 20 1.089838144307521 1105 21 1.1290517390507944 1105 22 1.0509646738204452 1105 23 1.0735460770953722 1105 24 1.226787617561051 1105 25 1.2729323226849303 1106 1 1.0771711395631414 1106 2 3.760524688872863 1106 3 2.2005301057975544 1106 4 1.1719060578880942 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0.8683464148849112 1107 18 0.6988164966917267 1107 19 0.8011997906992718 1107 20 0.8345015416090007 1107 21 0.851833292371424 1107 22 1.1289982942150019 1107 23 1.1575892111217598 1107 24 1.045371958716899 1107 25 1.1148843226456648 1108 1 1.1629772431652121 1108 2 3.75831276084617 1108 3 2.0940931718128084 1108 4 1.4548338888155072 1108 5 1.0296167537276872 1108 6 0.8834438746473054 1108 7 0.7956976402609897 1108 8 0.7381279660449351 1108 9 0.6918507267012046 1108 10 0.670515840346873 1108 11 0.8125983886478139 1108 12 0.7905155751924724 1108 13 0.669279323630974 1108 14 0.806487504761769 1108 15 0.7459210649612231 1108 16 0.7516742492816775 1108 17 0.8036161055466238 1108 18 0.7955579018849903 1108 19 0.8702721380325897 1108 20 0.7726573339879863 1108 21 0.6020398073322113 1108 22 0.638582724356759 1108 23 0.7781953412380247 1108 24 0.7601776999953245 1108 25 0.7189074987552146 1109 1 0.48164733753775124 1109 2 2.5464020651898593 1109 3 1.2670795074237553 1109 4 0.8868599835146895 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-0.00386590886667193 2314 1 -0.14046432872054382 2314 2 2.162792992972193 2314 3 0.8028793987616858 2314 4 0.1346947319406837 2314 5 -0.3093358610154908 2314 6 -0.4264296727332564 2314 7 -0.49120867252521805 2314 8 -0.4739710993412132 2314 9 -0.48498003315304317 2314 10 -0.5702771441686636 2314 11 -0.5042889898754908 2314 12 -0.6549044111075233 2314 13 -0.8494890437125093 2314 14 -1.6030308420091686 2314 15 -1.1274455777448438 2314 16 -1.047462492619779 2314 17 -0.8265795910888301 2314 18 -0.7315052432617861 2314 19 -0.8881159207940073 2314 20 -0.7458741202227728 2314 21 -0.58640345356946 2314 22 -0.5730002423968727 2314 23 -0.7228879586218611 2314 24 -0.6426180252726184 2314 25 -0.7721698378827497 2315 1 -4.52220544408161 2315 2 -13.62258507628766 2315 3 -7.053688891923414 2315 4 -3.182278745492237 2315 5 -2.0184315734449783 2315 6 -1.580175479345531 2315 7 -1.134857931037871 2315 8 -1.1059358177825587 2315 9 -1.1786304555734404 2315 10 -1.2920147693688122 2315 11 -1.2481682021864842 2315 12 -1.2251417123663657 2315 13 -1.2238650446536354 2315 14 -1.248166777840197 2315 15 -1.505987118549342 2315 16 -1.6192189668760975 2315 17 -1.4525499503426218 2315 18 -1.3158676975666879 2315 19 -1.3285004884085367 2315 20 -1.3328662248663576 2315 21 -1.4560573480281818 2315 22 -1.3119597963686758 2315 23 -1.3876181640273586 2315 24 -1.392410670031886 2315 25 -1.3916723071637875 2316 1 -1.7348517122756135 2316 2 -1.649317514097433 2316 3 -1.0786329511331374 2316 4 -1.2034294265532104 2316 5 -1.4201868310159185 2316 6 -1.5127325876128381 2316 7 -1.5457808069164223 2316 8 -1.7707564972345686 2316 9 -1.6113438291700106 2316 10 -1.9055339571972283 2316 11 -2.0308430784731613 2316 12 -2.008921512462905 2316 13 -1.8642728571352407 2316 14 -2.1757645221568396 2316 15 -2.7101534614618714 2316 16 -2.3329645863845343 2316 17 -2.352516750827668 2316 18 -2.191368260702667 2316 19 -2.288768910018831 2316 20 -2.3849624642168124 2316 21 -2.3840170690894666 2316 22 -2.426090467461947 2316 23 -2.3047655172882457 2316 24 -2.134802526339989 2316 25 -2.2294274548545374 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 80149.0 3 4.0 4 744.0 5 3600.0 6 4365.0 7 3909.0 8 3668.0 9 3444.0 10 3348.0 11 3219.0 12 3185.0 13 3207.0 14 3258.0 15 3337.0 16 3434.0 17 3782.0 18 4175.0 19 5258.0 20 6611.0 21 8625.0 22 10648.0 23 12728.0 24 14672.0 25 16823.0 26 19224.0 27 21423.0 28 24882.0 29 30233.0 30 37742.0 31 47486.0 32 61194.0 33 80287.0 34 109033.0 35 155359.0 36 238703.0 37 322475.0 38 747765.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.01933540406061 19.136973899606993 13.10875980435766 24.734930891974734 2 16.195992634066336 21.433296315724295 39.75751620233564 22.613194847873736 3 18.378981150799785 25.37670094039055 30.013953384373636 26.230364524436023 4 12.264515825174037 17.265184236529134 38.663624483170544 31.80667545512629 5 13.100159753984572 37.97301784696539 35.6847013913535 13.242121007696545 6 30.88968496370089 37.38736810055281 18.59189906659435 13.131047869151944 7 27.27684456557781 32.042736461815636 22.79358838895737 17.886830583649182 8 24.829269661879003 36.56010302573883 21.161427764713494 17.449199547668673 9 26.446587272401178 15.317752291984915 20.69929624133979 37.53636419427412 10 15.262519154385895 28.962715967038992 34.43008298291293 21.344681895662177 11 33.46899784443285 22.773987999042816 25.078984338241835 18.6780298182825 12 23.0766522724321 24.964997604661086 31.920329172373535 20.038020950533276 13 28.58481720002455 22.315540888022202 26.22682144646258 22.872820465490673 14 21.04829735884746 22.471276151891605 28.047587082581867 28.432839406679072 15 22.871989948615578 30.721565519298533 23.9323615282405 22.474083003845386 16 21.931599396574402 28.409766132143737 27.86775826249204 21.790876208789822 17 21.482979183961824 28.648967523555115 27.997821107191534 21.87023218529152 18 21.256480141046687 27.63469440280419 29.959909590822832 21.14891586532629 19 23.172887039002546 27.08175249085272 28.05835039255639 21.687010077588344 20 23.283149095047165 27.24925166080872 28.627994070375284 20.83960517376883 21 23.07239255864935 26.76300105043616 27.708346019760377 22.45626037115411 22 23.10238732645984 27.737594385246428 27.908440542349723 21.251577745944008 23 22.378047881225104 27.51455404466142 28.27288197439287 21.8345160997206 24 22.868526844120936 26.880515711601134 28.72204381645615 21.528913627821787 25 22.623173561220817 27.21956942358441 28.405027086456393 21.75222992873838 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 361.0 1 361.0 2 550.0 3 739.0 4 739.0 5 739.0 6 1795.0 7 2851.0 8 2851.0 9 2851.0 10 4074.5 11 5298.0 12 5298.0 13 5298.0 14 8413.0 15 11528.0 16 11528.0 17 11528.0 18 20079.5 19 28631.0 20 28631.0 21 28631.0 22 43502.0 23 58373.0 24 58373.0 25 58373.0 26 82717.0 27 107061.0 28 107061.0 29 107061.0 30 136672.0 31 166283.0 32 166283.0 33 166283.0 34 186280.5 35 206278.0 36 206278.0 37 206278.0 38 220327.0 39 234376.0 40 234376.0 41 234376.0 42 244916.0 43 255456.0 44 255456.0 45 255456.0 46 261049.0 47 266642.0 48 266642.0 49 266642.0 50 257749.5 51 248857.0 52 248857.0 53 248857.0 54 222366.0 55 195875.0 56 195875.0 57 195875.0 58 167540.0 59 139205.0 60 139205.0 61 139205.0 62 114411.5 63 89618.0 64 89618.0 65 89618.0 66 69029.0 67 48440.0 68 48440.0 69 48440.0 70 35529.5 71 22619.0 72 22619.0 73 22619.0 74 15788.0 75 8957.0 76 8957.0 77 8957.0 78 6049.5 79 3142.0 80 3142.0 81 3142.0 82 2083.0 83 1024.0 84 1024.0 85 1024.0 86 655.0 87 286.0 88 286.0 89 286.0 90 178.0 91 70.0 92 70.0 93 70.0 94 45.0 95 20.0 96 20.0 97 20.0 98 14.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.497481207174694 3 0.0 4 0.0 5 8.087539527849442E-4 6 9.039014766419965E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 9.514752385705226E-5 14 0.0 15 0.0 16 0.0 17 6.660326669993658E-4 18 0.0 19 3.8059009542820905E-4 20 5.233113812137875E-4 21 7.13606428927892E-4 22 0.005137966288280822 23 2.854425715711568E-4 24 0.0023311143344977806 25 0.0012369178101416794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2101999.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.55532080630496 #Duplication Level Percentage of deduplicated Percentage of total 1 83.63030688959984 60.678237455046535 2 11.248818725904043 16.32323302699877 3 2.750769509507079 5.987488926794649 4 0.9842218194926802 2.8564211943142634 5 0.44617845266496536 1.6186310384983658 6 0.24772165939936416 1.0784114681034649 7 0.15477097775091944 0.7860620559556414 8 0.09371476227764058 0.5439603713072656 9 0.07364258116445145 0.4808844991252015 >10 0.3350176905566305 4.112037511912966 >50 0.01831016022795862 0.9312255079559909 >100 0.013542055842039264 1.9098709932461815 >500 0.002056137421874963 1.0069419205178463 >1k 8.622511769150971E-4 1.3742707142144344 >5k 6.632701360885362E-5 0.312323316008393 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6490 0.3087537149161346 No Hit GGTATCAACGCAGAGTACTTTTTTT 4300 0.20456717629266236 No Hit TATCAACGCAGAGTACTTTTTTTTT 3990 0.18981931009481925 No Hit GTCCTACAGTGGACATTTCTAAATT 2865 0.13629882792522738 No Hit CTGTAGGACGTGGAATATGGCAAGA 2757 0.13116086163694654 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2495 0.1186965360116727 No Hit CTTTAGGACGTGAAATATGGCGAGG 2325 0.11060899648382325 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.757376192852613E-5 2 0.0 4.757376192852613E-5 0.0 0.0 4.757376192852613E-5 3 0.0 4.757376192852613E-5 0.0 0.0 4.757376192852613E-5 4 0.0 4.757376192852613E-5 0.0 0.0 4.757376192852613E-5 5 0.0 9.514752385705226E-5 0.0 0.0 4.757376192852613E-5 6 0.0 9.514752385705226E-5 0.0 0.0 4.757376192852613E-5 7 0.0 9.514752385705226E-5 0.0 0.0 4.757376192852613E-5 8 0.0 9.514752385705226E-5 0.0 0.0 4.757376192852613E-5 9 0.0 9.514752385705226E-5 0.0 0.0 4.757376192852613E-5 10 0.0 1.427212857855784E-4 0.0 4.757376192852613E-5 4.757376192852613E-5 11 0.0 1.427212857855784E-4 0.0 4.757376192852613E-5 4.757376192852613E-5 12 0.0 1.427212857855784E-4 0.0 4.757376192852613E-5 9.514752385705226E-5 13 0.0 1.427212857855784E-4 0.0 4.757376192852613E-5 1.9029504771410452E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1415 0.0 14.197188 1 TAGGACC 555 0.0 12.082916 4 AGCCGTT 55 0.0032057574 12.020747 7 TCCAACG 135 5.178663E-9 11.194455 18 GGCGAGG 875 0.0 11.010547 19 GTGGTAT 425 0.0 10.817617 1 AGATCGG 80 3.9801942E-4 10.625482 10 GCGCCAC 80 3.9801942E-4 10.625482 13 GTCCTAA 970 0.0 10.612757 1 TTAGGAC 1195 0.0 10.591155 3 TGGCGAG 1810 0.0 10.489004 18 GTTTAGG 230 0.0 10.429082 1 AGGACGT 1870 0.0 10.404759 5 GGACGTG 1845 0.0 10.340728 6 GTATCAA 2875 0.0 10.324792 1 GACGTGA 960 0.0 10.231946 7 CGAGTAA 75 0.002779183 10.074531 10 GTCTAGA 230 1.8189894E-12 9.994537 1 GTCTTAA 250 0.0 9.994537 1 TGGTATC 440 0.0 9.767389 2 >>END_MODULE