FastQCFastQC Report
Thu 26 May 2016
SRR1511784_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511784_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101999
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82430.3921505195768409No Hit
GGTATCAACGCAGAGTACTTTTTTT55540.26422467375103414No Hit
TATCAACGCAGAGTACTTTTTTTTT54130.25751677331911194No Hit
CTGTAGGACGTGGAATATGGCAAGA30340.14433879369114827No Hit
GTCCTACAGTGGACATTTCTAAATT30090.14314944964293513No Hit
CTTTAGGACGTGAAATATGGCGAGG26380.12549958396745195No Hit
GTCCTAAAGTGTGTATTTCTCATTT26100.1241675186334532No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25500.12131309291774164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16950.013.2229381
AAGTCGT1201.0059011E-811.8718337
TAGGACC5100.011.7346764
GTGGTAT5400.011.4801991
GCGTTGA752.0001356E-411.4448751
GGCGAGG8500.011.39723119
TTAGGAC11500.010.4907523
TATACTG2900.010.4822075
TCCAACG1451.44991645E-810.47996318
TGGCGAG17950.010.47631518
TGGTATC5750.010.077732
ACCGTCT856.6111924E-410.0561428
GGTTAGT951.5805203E-410.0393641
GTATAAG3100.09.8450541
GTATCAA34800.09.8388661
AGGACCT11300.09.8357675
ATGGCGA19000.09.69741317
TATGGCG18650.09.62477816
AGGACGT17000.09.6112595
GTCCTAC14950.09.5054981
Produced by FastQC (version 0.11.5)