##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511784_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2101999 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.322099106612324 33.0 31.0 34.0 28.0 34.0 2 31.512263326481126 33.0 31.0 34.0 28.0 34.0 3 31.625072133716525 34.0 31.0 34.0 28.0 34.0 4 35.182961552312825 37.0 35.0 37.0 32.0 37.0 5 34.87593143479136 37.0 35.0 37.0 32.0 37.0 6 34.82730724419945 37.0 35.0 37.0 32.0 37.0 7 34.695195858799174 37.0 35.0 37.0 32.0 37.0 8 34.622969849176904 37.0 35.0 37.0 32.0 37.0 9 36.19551008349671 39.0 37.0 39.0 32.0 39.0 10 36.03676119731741 39.0 37.0 39.0 31.0 39.0 11 36.02177165640897 39.0 37.0 39.0 31.0 39.0 12 35.87523210049101 39.0 37.0 39.0 30.0 39.0 13 35.860409067749316 39.0 37.0 39.0 30.0 39.0 14 36.976487619642064 40.0 37.0 41.0 31.0 41.0 15 36.90985009983354 40.0 37.0 41.0 31.0 41.0 16 36.82582294282728 40.0 37.0 41.0 31.0 41.0 17 36.788238719428506 40.0 37.0 41.0 30.0 41.0 18 36.72355981139858 40.0 37.0 41.0 30.0 41.0 19 36.75667495560179 40.0 37.0 41.0 30.0 41.0 20 36.63713541252874 40.0 37.0 41.0 30.0 41.0 21 36.54661919439543 40.0 37.0 41.0 30.0 41.0 22 36.62759497031159 40.0 37.0 41.0 30.0 41.0 23 36.53790130252203 40.0 37.0 41.0 30.0 41.0 24 36.427722848583656 40.0 37.0 41.0 30.0 41.0 25 36.0170927769233 39.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34255137298625726 1101 2 0.3401754866544131 1101 3 0.34263013362356176 1101 4 0.36998008476066957 1101 5 0.49574629946143034 1101 6 0.5069865139344003 1101 7 0.4367653539042635 1101 8 0.300168461139819 1101 9 0.4121731681351335 1101 10 0.37611595826182764 1101 11 0.18878631227562437 1101 12 0.3532632029031717 1101 13 0.3688978598873831 1101 14 0.4708135611123936 1101 15 0.28961262199153737 1101 16 0.35399316348249243 1101 17 0.5357309457827952 1101 18 0.5055715337082844 1101 19 0.41248615859545623 1101 20 0.4043101907686122 1101 21 0.4632538925068772 1101 22 0.22918786692741122 1101 23 0.36163364537799936 1101 24 0.24872655224273643 1101 25 0.375494658762733 1102 1 0.37656815561412316 1102 2 0.347823625980034 1102 3 0.32318460981576536 1102 4 0.24971903077040736 1102 5 0.42500093749469414 1102 6 0.30530743125432025 1102 7 0.4823039783401839 1102 8 0.44503304553157363 1102 9 0.3585114519639987 1102 10 0.5879356555514121 1102 11 0.6321089996863734 1102 12 0.6205466244579867 1102 13 0.7203368749145582 1102 14 0.9122203338400539 1102 15 0.9438976326750392 1102 16 0.8961419688249208 1102 17 1.0392657102787268 1102 18 0.8941537597709868 1102 19 0.8617949447172393 1102 20 0.8947702943269746 1102 21 0.9355703455084097 1102 22 0.6500000941116184 1102 23 0.7795542849883859 1102 24 0.8302753246137371 1102 25 0.95096392316335 1103 1 0.2635235315927069 1103 2 0.2890640153801627 1103 3 0.3539200265846425 1103 4 0.38352885828773964 1103 5 0.4253320257264832 1103 6 0.5176706751340276 1103 7 0.3701373969453243 1103 8 0.29786166321718355 1103 9 0.546838979805834 1103 10 0.539292879007931 1103 11 0.5286880816944901 1103 12 0.5099721261015162 1103 13 0.5669109807870996 1103 14 0.7707612748202806 1103 15 0.7429108159299957 1103 16 0.5593615986284632 1103 17 0.6066968328528617 1103 18 0.6218060750757601 1103 19 0.8291444867025106 1103 20 0.6553985489649463 1103 21 0.5237523739494776 1103 22 0.6262130446796661 1103 23 0.716094398948897 1103 24 0.7543893972653208 1103 25 0.6398194820674803 1104 1 0.3765706708853145 1104 2 0.3708920164831504 1104 3 0.3896076119149008 1104 4 0.35111687876027275 1104 5 0.3859575007175735 1104 6 0.416018210560793 1104 7 0.42214651677422665 1104 8 0.5559011690246791 1104 9 0.682691340805782 1104 10 0.6202468292524017 1104 11 0.7314051988600738 1104 12 0.70387448474753 1104 13 0.8699362620827102 1104 14 0.8930587184434629 1104 15 0.8354609379984979 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2314 22 -0.7364822340266457 2314 23 -0.8341716377341655 2314 24 -0.8533131248873431 2314 25 -0.7826009040107706 2315 1 -0.4357876368266673 2315 2 -0.4276817675725155 2315 3 -0.481077271004807 2315 4 -0.4359548526109549 2315 5 -0.4797584921583251 2315 6 -0.6326770350234341 2315 7 -0.6072105345524292 2315 8 -0.7293531037835521 2315 9 -0.8330075232749152 2315 10 -0.8287476417079773 2315 11 -0.7246374275168748 2315 12 -0.7530466466437744 2315 13 -0.8801224930715819 2315 14 -1.0151083608947715 2315 15 -1.053023729112752 2315 16 -0.9655002695865562 2315 17 -1.0851987151187856 2315 18 -1.0188379737014657 2315 19 -1.1357880045082211 2315 20 -1.1093898136831086 2315 21 -1.1666460900518416 2315 22 -1.1501130153086976 2315 23 -1.1735735391375854 2315 24 -0.9908804577310519 2315 25 -1.0436772827610312 2316 1 -1.8171091030993338 2316 2 -1.3584658804665644 2316 3 -1.1608234477627946 2316 4 -1.0619179721095833 2316 5 -1.172775213568869 2316 6 -1.1437514670801932 2316 7 -1.2009671420800032 2316 8 -1.2786022688903174 2316 9 -1.5588675908512357 2316 10 -1.6269614607117546 2316 11 -1.406886047146969 2316 12 -1.3892177928903848 2316 13 -1.5553932496359408 2316 14 -1.7196797692841272 2316 15 -1.6771174726131477 2316 16 -1.6913777610217835 2316 17 -1.787990064408298 2316 18 -1.906181580832687 2316 19 -1.8765363670971524 2316 20 -1.8160068772919686 2316 21 -2.0715568712607606 2316 22 -2.040445049701198 2316 23 -1.884404508084657 2316 24 -1.755632462063474 2316 25 -1.8662882715650255 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22851.0 3 0.0 4 389.0 5 3836.0 6 4905.0 7 4095.0 8 3154.0 9 3009.0 10 2768.0 11 2704.0 12 2496.0 13 2496.0 14 2483.0 15 2406.0 16 2567.0 17 2742.0 18 2980.0 19 3340.0 20 4104.0 21 5209.0 22 6907.0 23 8703.0 24 10639.0 25 12694.0 26 15004.0 27 17178.0 28 19957.0 29 24967.0 30 31837.0 31 41515.0 32 53917.0 33 70994.0 34 94567.0 35 127972.0 36 189070.0 37 346346.0 38 951198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.09337755905907 19.672167173625464 13.611640735887285 24.62281453142818 2 14.86984532342784 21.02436775659741 40.739124994826355 23.366661925148392 3 18.308667130669427 25.664855216391636 30.390119119942494 25.636358532996447 4 12.60657117344014 17.721559334709482 38.35011339206156 31.32175609978882 5 13.052737274907678 38.12193055925686 35.72491148589485 13.10042067994061 6 30.863953788750614 37.61747745836225 18.447725236786503 13.070843516100625 7 27.28383790858131 32.10729405675264 22.726176368304646 17.882691666361403 8 24.938689314314612 36.69535523090163 20.955433375562976 17.41052207922078 9 26.426320849819625 15.192395429303248 20.65119916803005 37.730084552847075 10 15.267133809292963 29.114523841352923 34.32856057495746 21.28978177439666 11 33.54378379818449 22.77964927671231 25.01780448040175 18.65876244470145 12 22.98550094457704 25.049441032084218 31.97332634316191 19.991731680176823 13 28.630984125111382 22.301104805473265 26.13245772238712 22.935453347028233 14 21.034833984221688 22.388688101183682 28.109861136946307 28.466616777648323 15 22.83202798859562 30.65244084321639 24.006433875563214 22.509097292624784 16 21.845331770374443 28.44425013177951 27.874410798117598 21.83600729972845 17 21.46494836581749 28.6433533032128 28.00410466417919 21.887593666790515 18 21.239326221683474 27.524064021023815 30.026731722262213 21.209878035030496 19 23.07170460119153 27.062762636899446 28.261621437498306 21.603911324410717 20 23.206576216258902 27.154151833564143 28.729033648446073 20.910238301730878 21 22.9717521273797 26.71014591348521 27.93602661085947 22.382075348275617 22 23.02622408478786 27.589594476495943 28.110812612184876 21.273368826531318 23 22.349249452544935 27.35672091185581 28.36809151669435 21.92593811890491 24 22.73004726664621 26.91724887661431 28.829514178804466 21.52318967793502 25 22.508912965498475 27.165824450497954 28.580528774106874 21.7447338098967 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 352.0 1 352.0 2 538.0 3 724.0 4 724.0 5 724.0 6 1869.5 7 3015.0 8 3015.0 9 3015.0 10 4186.0 11 5357.0 12 5357.0 13 5357.0 14 8247.5 15 11138.0 16 11138.0 17 11138.0 18 19678.0 19 28218.0 20 28218.0 21 28218.0 22 43633.0 23 59048.0 24 59048.0 25 59048.0 26 84108.0 27 109168.0 28 109168.0 29 109168.0 30 137994.0 31 166820.0 32 166820.0 33 166820.0 34 186577.0 35 206334.0 36 206334.0 37 206334.0 38 220290.0 39 234246.0 40 234246.0 41 234246.0 42 245276.5 43 256307.0 44 256307.0 45 256307.0 46 261189.0 47 266071.0 48 266071.0 49 266071.0 50 257184.0 51 248297.0 52 248297.0 53 248297.0 54 221684.5 55 195072.0 56 195072.0 57 195072.0 58 167004.5 59 138937.0 60 138937.0 61 138937.0 62 114427.0 63 89917.0 64 89917.0 65 89917.0 66 69031.5 67 48146.0 68 48146.0 69 48146.0 70 35071.5 71 21997.0 72 21997.0 73 21997.0 74 15288.5 75 8580.0 76 8580.0 77 8580.0 78 5818.5 79 3057.0 80 3057.0 81 3057.0 82 1991.5 83 926.0 84 926.0 85 926.0 86 565.0 87 204.0 88 204.0 89 204.0 90 133.0 91 62.0 92 62.0 93 62.0 94 33.5 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41855395744717294 2 0.0 3 0.0 4 0.0 5 0.030399633872328195 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.427212857855784E-4 17 0.0 18 9.514752385705226E-5 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004471933621281456 25 2.3786880964263063E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2101999.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.6344351860454 #Duplication Level Percentage of deduplicated Percentage of total 1 81.69593877393181 56.88850553516488 2 12.411870521006124 17.28587186665177 3 3.134885936573776 6.548880346979755 4 1.1235942709756825 3.1296340975067234 5 0.5445088877507914 1.8958284426154077 6 0.2762849438596305 1.1543367609644175 7 0.18113323120477193 0.8829177173857364 8 0.12213145477948026 0.6803643897615321 9 0.0887134744545208 0.5559761418329 >10 0.380514915476581 4.522989744408584 >50 0.020699641022904405 0.9879953654501401 >100 0.016041228739175767 2.091182327266697 >500 0.002455146816456263 1.1643988772535059 >1k 0.001022977840189976 1.2988420935959955 >5k 2.045955680379952E-4 0.9122762931619405 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8243 0.3921505195768409 No Hit GGTATCAACGCAGAGTACTTTTTTT 5554 0.26422467375103414 No Hit TATCAACGCAGAGTACTTTTTTTTT 5413 0.25751677331911194 No Hit CTGTAGGACGTGGAATATGGCAAGA 3034 0.14433879369114827 No Hit GTCCTACAGTGGACATTTCTAAATT 3009 0.14314944964293513 No Hit CTTTAGGACGTGAAATATGGCGAGG 2638 0.12549958396745195 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2610 0.1241675186334532 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2550 0.12131309291774164 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.757376192852613E-5 0.0 0.0 0.0 6 0.0 4.757376192852613E-5 0.0 0.0 0.0 7 0.0 9.514752385705226E-5 0.0 0.0 0.0 8 0.0 9.514752385705226E-5 0.0 0.0 4.757376192852613E-5 9 0.0 9.514752385705226E-5 0.0 0.0 4.757376192852613E-5 10 0.0 9.514752385705226E-5 0.0 1.9029504771410452E-4 4.757376192852613E-5 11 0.0 9.514752385705226E-5 0.0 2.3786880964263066E-4 4.757376192852613E-5 12 0.0 9.514752385705226E-5 0.0 2.3786880964263066E-4 4.757376192852613E-5 13 0.0 9.514752385705226E-5 0.0 2.3786880964263066E-4 4.757376192852613E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1695 0.0 13.222938 1 AAGTCGT 120 1.0059011E-8 11.871833 7 TAGGACC 510 0.0 11.734676 4 GTGGTAT 540 0.0 11.480199 1 GCGTTGA 75 2.0001356E-4 11.444875 1 GGCGAGG 850 0.0 11.397231 19 TTAGGAC 1150 0.0 10.490752 3 TATACTG 290 0.0 10.482207 5 TCCAACG 145 1.44991645E-8 10.479963 18 TGGCGAG 1795 0.0 10.476315 18 TGGTATC 575 0.0 10.07773 2 ACCGTCT 85 6.6111924E-4 10.056142 8 GGTTAGT 95 1.5805203E-4 10.039364 1 GTATAAG 310 0.0 9.845054 1 GTATCAA 3480 0.0 9.838866 1 AGGACCT 1130 0.0 9.835767 5 ATGGCGA 1900 0.0 9.697413 17 TATGGCG 1865 0.0 9.624778 16 AGGACGT 1700 0.0 9.611259 5 GTCCTAC 1495 0.0 9.505498 1 >>END_MODULE