FastQCFastQC Report
Thu 26 May 2016
SRR1511783_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511783_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658816
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT56850.3427143215401829No Hit
CTGTAGGACGTGGAATATGGCAAGA52930.319083008603727No Hit
GTCCTAAAGTGTGTATTTCTCATTT51390.30979927852154787No Hit
GTATCAACGCAGAGTACTTTTTTTT48800.2941857324742467No Hit
CTTTAGGACGTGAAATATGGCGAGG46950.28303319958331724No Hit
GGTATCAACGCAGAGTACTTTTTTT33220.2002633203441491No Hit
TATCAACGCAGAGTACTTTTTTTTT29680.17892279794745167No Hit
GTCCTACAGTGTGCATTTCTCATTT29400.17723484702341913No Hit
CTGAAGGACCTGGAATATGGCGAGA23860.14383753231220342No Hit
CTGTAGGACCTGGAATATGGCGAGA21240.12804313438018441No Hit
ATTTAGAAATGTCCACTGTAGGACG20800.1253906400709904No Hit
TTTCTAAATTTTCCACCTTTTTCAG19910.12002536749102974No Hit
GTCCTTCAGTGTGCATTTCTCATTT19630.1183374165669972No Hit
GAATATGGCAAGAAAACTGAAAATC17780.10718488367606775No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAT401.8838735E-417.478471
GTCAACG400.003785607214.9815452
CGTTAAA457.064107E-414.6930059
TAGGACC9100.013.5981924
GGTATCA9050.012.4708261
CGGTAGG1051.418739E-712.3657211
CAAGTCG2550.011.853186
CTAGTAC1201.08939275E-811.80759053
GGCGAGG15100.011.76067319
GTGGTAT3000.011.6523131
ACACCGT658.4205595E-411.6252346
AGTCGTC2950.011.5267158
CCAACGT752.1865497E-411.33528719
ACCTTTT8350.011.31197915
AGGACCT18450.011.1612125
ATCCCGT1201.3807221E-711.019752510
TAGGACA8750.010.9034664
TCCAACG1658.913048E-1110.877295518
TGTAGGA28800.010.8200052
ACGTTAA700.001564799710.794868
Produced by FastQC (version 0.11.5)