##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511783_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1658816 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.951839143099658 31.0 30.0 34.0 25.0 34.0 2 27.18292746151472 31.0 27.0 34.0 10.0 34.0 3 28.95224605984027 31.0 28.0 34.0 16.0 34.0 4 33.36736805046491 35.0 33.0 37.0 28.0 37.0 5 33.74812094891778 37.0 35.0 37.0 30.0 37.0 6 33.96162142154404 37.0 35.0 37.0 30.0 37.0 7 33.957289416065436 37.0 35.0 37.0 30.0 37.0 8 33.97525644797253 37.0 35.0 37.0 30.0 37.0 9 35.580123413326135 39.0 37.0 39.0 30.0 39.0 10 35.453829116671166 39.0 35.0 39.0 30.0 39.0 11 35.45968932057564 39.0 35.0 39.0 30.0 39.0 12 35.35547161348818 39.0 35.0 39.0 30.0 39.0 13 35.356287255488255 39.0 35.0 39.0 30.0 39.0 14 36.50542133666422 40.0 37.0 41.0 30.0 41.0 15 36.46747981692967 40.0 37.0 41.0 29.0 41.0 16 36.43180256279177 40.0 37.0 41.0 29.0 41.0 17 36.39876032061422 40.0 37.0 41.0 29.0 41.0 18 36.371890553262084 40.0 37.0 41.0 29.0 41.0 19 36.397204391566035 40.0 37.0 41.0 29.0 41.0 20 36.34969821848837 40.0 37.0 41.0 29.0 41.0 21 36.23800710868475 40.0 37.0 41.0 29.0 41.0 22 36.1539537839037 40.0 37.0 41.0 29.0 41.0 23 36.05109970002701 40.0 36.0 41.0 28.0 41.0 24 35.9662620809059 40.0 36.0 41.0 28.0 41.0 25 35.877880367683936 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0206968363171711 1101 2 3.720193766477827 1101 3 2.237858420632328 1101 4 1.3027944027542446 1101 5 0.698692775210688 1101 6 0.7821787195521424 1101 7 0.6374452835776978 1101 8 0.5646659633604187 1101 9 0.6410360318811428 1101 10 0.572391986152148 1101 11 0.5909674451931295 1101 12 0.6134582856775879 1101 13 0.49317501805833075 1101 14 0.5367413047212892 1101 15 0.6553088849534561 1101 16 0.5963584826973047 1101 17 0.7183769856079323 1101 18 0.8169632877566002 1101 19 0.8426032427467405 1101 20 0.7735089797172563 1101 21 0.9797549610334997 1101 22 0.7749593081375963 1101 23 0.7640708780210232 1101 24 0.7875827953113372 1101 25 0.8799349924619619 1102 1 1.103030887471352 1102 2 3.9168011184745026 1102 3 2.168467812751409 1102 4 1.2385418809362676 1102 5 0.8803165615267545 1102 6 0.7696395227558881 1102 7 0.49703196988998855 1102 8 0.6152685550714736 1102 9 0.7081673930961543 1102 10 0.6404791824950422 1102 11 0.6199069593417548 1102 12 0.7582843085722928 1102 13 0.7427688825790923 1102 14 0.861785696273671 1102 15 0.7222248999143517 1102 16 0.7816733793688826 1102 17 0.9733280604501999 1102 18 0.85885757919187 1102 19 0.9968667207776392 1102 20 0.9519630202930287 1102 21 0.9416348960518235 1102 22 1.1507989696283971 1102 23 1.0284858540306985 1102 24 0.7690819074802846 1102 25 0.9381313731336931 1103 1 0.9778863788008323 1103 2 3.631184913991685 1103 3 2.0701120815903806 1103 4 1.2646542521792412 1103 5 0.6767849403467849 1103 6 0.5195163550282729 1103 7 0.6331514141311843 1103 8 0.3765837262461602 1103 9 0.3321794090632437 1103 10 0.571866628329694 1103 11 0.6836289823818404 1103 12 0.45834121775337167 1103 13 0.5936176423654373 1103 14 0.6851404111993205 1103 15 0.5584362118335733 1103 16 0.4750215090892951 1103 17 0.6838936564112714 1103 18 0.7133643798390068 1103 19 0.6719612306676481 1103 20 0.6553572464501087 1103 21 0.8273722357915076 1103 22 0.8738134489500382 1103 23 0.9005853150194554 1103 24 0.8929439271058257 1103 25 0.8282844588307938 1104 1 1.2568094557239107 1104 2 3.819821720223427 1104 3 2.1930741069068382 1104 4 1.4301810291996944 1104 5 0.9750205781267312 1104 6 1.0183556929055868 1104 7 0.9239128552217366 1104 8 0.9360365013191938 1104 9 0.993782135450779 1104 10 0.9788676020395286 1104 11 0.9479950972796019 1104 12 1.0631435546569747 1104 13 1.0253157923167535 1104 14 1.1646146078887085 1104 15 1.0372093374421425 1104 16 1.1113408859150056 1104 17 1.0179345522243182 1104 18 1.0610839514066797 1104 19 1.040161814893544 1104 20 0.8906834392446612 1104 21 1.1346146895402853 1104 22 0.9916732347338737 1104 23 1.1600809333251973 1104 24 1.1757637907864478 1104 25 1.2108550527937894 1105 1 0.997337750371905 1105 2 3.719932980534189 1105 3 2.007622735335765 1105 4 1.2528025523149822 1105 5 0.813113422937775 1105 6 0.5843921402487666 1105 7 0.450686689865897 1105 8 0.44640442765719257 1105 9 0.5314660027057201 1105 10 0.3144689832020049 1105 11 0.5278001999076452 1105 12 0.30507564750178773 1105 13 0.44511705838235827 1105 14 0.6463276330181955 1105 15 0.5721124642405329 1105 16 0.5439804408737601 1105 17 0.5250628852379435 1105 18 0.5751829347176312 1105 19 0.526724638603504 1105 20 0.6554503680764228 1105 21 0.7120418907679849 1105 22 0.7121416790078072 1105 23 0.7103709774456703 1105 24 0.888565632336416 1105 25 0.833414039941303 1106 1 0.8242451111002254 1106 2 3.643683953741764 1106 3 1.9062716793950365 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0.9634482721996065 1107 17 1.0298249403172406 1107 18 0.8961853563308679 1107 19 1.0080371800347336 1107 20 0.9586724966008049 1107 21 1.0235446287993426 1107 22 1.0417760770392448 1107 23 1.1392531872316312 1107 24 1.064613607636268 1107 25 1.2252103177819649 1108 1 0.8196769796336696 1108 2 3.6571749440370915 1108 3 1.9503043270139173 1108 4 1.1168993745069713 1108 5 0.6634188758432913 1108 6 0.5497101064872396 1108 7 0.5333592470214938 1108 8 0.490856800591601 1108 9 0.41187466279256313 1108 10 0.5334111429721204 1108 11 0.4655324605706852 1108 12 0.4033234841861031 1108 13 0.47249210678158704 1108 14 0.5910787089274692 1108 15 0.5605333661796195 1108 16 0.4998733528531787 1108 17 0.4283111595551219 1108 18 0.672839225282118 1108 19 0.5201193493281764 1108 20 0.5735132566610304 1108 21 0.5493388921669293 1108 22 0.6896603303271576 1108 23 0.6470288123349448 1108 24 0.6570897414568364 1108 25 0.507171348039293 1109 1 0.6848968075377968 1109 2 2.8086032515542065 1109 3 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-0.250449452873589 2314 1 0.13811844631138115 2314 2 2.3831891334009683 2314 3 1.0208504782148538 2314 4 0.3254137458501276 2314 5 -0.04737117779667699 2314 6 -0.1599140247185673 2314 7 -0.18921145506713088 2314 8 -0.2571715480154424 2314 9 -0.37256742637979556 2314 10 -0.3392093210373943 2314 11 -0.32059042690085704 2314 12 -0.4457727062972623 2314 13 -0.6196734968750661 2314 14 -1.2184460866909745 2314 15 -0.7933570371115692 2314 16 -0.8397464234001504 2314 17 -0.6902624617321891 2314 18 -0.5006651560259741 2314 19 -0.6396421448597849 2314 20 -0.6037144835076944 2314 21 -0.4733661608329669 2314 22 -0.33947769029047237 2314 23 -0.5504695373012183 2314 24 -0.4940813893498728 2314 25 -0.3462936002409407 2315 1 -4.53771738492107 2315 2 -13.892203692941557 2315 3 -7.295795731044027 2315 4 -3.3715927453791004 2315 5 -2.0322147751243094 2315 6 -1.539373849898709 2315 7 -1.27202039500947 2315 8 -1.2935576118337337 2315 9 -1.2830988006544146 2315 10 -1.466300277650042 2315 11 -1.405653541588066 2315 12 -1.3468087554479737 2315 13 -1.1872656544643831 2315 14 -1.3550701401912306 2315 15 -1.4006611036692789 2315 16 -1.4124115769906211 2315 17 -1.4046365201951545 2315 18 -1.4124443770343547 2315 19 -1.5685970415140318 2315 20 -1.5233163194911157 2315 21 -1.6360932601159135 2315 22 -1.6057140345572094 2315 23 -1.54247019458505 2315 24 -1.769727865492456 2315 25 -1.6890122518827155 2316 1 -1.3157283398138873 2316 2 -1.361187270785564 2316 3 -0.8638349840022528 2316 4 -0.8115252645065993 2316 5 -1.0311922090860577 2316 6 -0.9925623890124982 2316 7 -1.1784574411485593 2316 8 -1.3948366255539355 2316 9 -1.375889526220881 2316 10 -1.6324993643274368 2316 11 -1.594564343279842 2316 12 -1.407202099688682 2316 13 -1.297621603620641 2316 14 -1.5771369738628707 2316 15 -2.3505862003533906 2316 16 -1.812327112752996 2316 17 -1.7500191607293942 2316 18 -1.7230609037381797 2316 19 -1.854489533757807 2316 20 -2.060821722260499 2316 21 -1.974153875228275 2316 22 -2.0227000586394226 2316 23 -1.9148867656424997 2316 24 -1.848806305783647 2316 25 -2.1140616971229633 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48922.0 3 1.0 4 439.0 5 2235.0 6 2780.0 7 2431.0 8 2202.0 9 2230.0 10 2050.0 11 2007.0 12 1999.0 13 1927.0 14 2048.0 15 2098.0 16 2250.0 17 2367.0 18 2679.0 19 3332.0 20 4222.0 21 5439.0 22 6899.0 23 8219.0 24 9455.0 25 11189.0 26 12715.0 27 14311.0 28 16628.0 29 20332.0 30 26050.0 31 33572.0 32 43597.0 33 58508.0 34 81101.0 35 118938.0 36 190274.0 37 257140.0 38 656230.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.01279707936263 19.7912245842818 12.962619121108068 25.233359215247503 2 15.24719128338558 21.403676285928817 41.86654564507102 21.482586785614576 3 18.332292430263514 25.577158648095992 30.364850592229637 25.725698329410857 4 11.755915062309501 17.39011439484548 39.89122361973841 30.9627469231066 5 12.167274936972586 38.6413809484194 36.8035485850632 12.387795529544816 6 30.025132519937518 38.496280760811835 18.924323323930995 12.554263395319648 7 26.624351344573476 31.96707772290598 23.568557332458813 17.84001360006173 8 23.365038678189745 39.295738647324356 21.2774050889309 16.061817585554998 9 26.087100679038542 15.181732030556736 21.354930263513253 37.37623702689147 10 14.678722655195031 29.19057930475713 35.92616661522435 20.20453142482349 11 33.03482725027972 22.07568530807516 26.86156873336163 18.0279187082835 12 23.518823064161428 24.625455746749488 33.311892337667345 18.543828851421736 13 29.042918615348075 22.894188257393537 26.25375720243499 21.809135924823398 14 19.601390389289712 23.815842142829585 28.47880657046954 28.103960897411167 15 21.50889550136965 33.93022493151742 23.582724063428373 20.978155503684555 16 20.192233496662677 28.52088477564721 30.607312685674604 20.679569042015512 17 20.253688037622172 29.940276427243884 29.365104722725764 20.44093081240818 18 20.05128959450596 27.433060689069794 32.91100399320961 19.60464572321463 19 22.99162111702766 26.886816054612545 28.63475268158617 21.486810146773628 20 23.046433230528837 28.065446837857454 29.569233885455375 19.318886046158333 21 21.58189590470742 27.25555173085235 28.109478679557053 23.05307368488317 22 22.40213898585062 29.800088019147182 28.254688828199885 19.543084166802313 23 20.55098501275914 29.367661535883233 29.55930482737334 20.522048623984286 24 22.658114144818146 27.16555037768949 30.338202402985342 19.838133074507013 25 21.518619528863102 28.425014287471157 29.842608735492487 20.213757448173254 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 253.0 1 253.0 2 373.5 3 494.0 4 494.0 5 494.0 6 1377.0 7 2260.0 8 2260.0 9 2260.0 10 3222.5 11 4185.0 12 4185.0 13 4185.0 14 6694.5 15 9204.0 16 9204.0 17 9204.0 18 16228.5 19 23253.0 20 23253.0 21 23253.0 22 37743.5 23 52234.0 24 52234.0 25 52234.0 26 76276.5 27 100319.0 28 100319.0 29 100319.0 30 130178.0 31 160037.0 32 160037.0 33 160037.0 34 174905.0 35 189773.0 36 189773.0 37 189773.0 38 196251.5 39 202730.0 40 202730.0 41 202730.0 42 206824.0 43 210918.0 44 210918.0 45 210918.0 46 210986.5 47 211055.0 48 211055.0 49 211055.0 50 196265.0 51 181475.0 52 181475.0 53 181475.0 54 154283.5 55 127092.0 56 127092.0 57 127092.0 58 106429.5 59 85767.0 60 85767.0 61 85767.0 62 68937.5 63 52108.0 64 52108.0 65 52108.0 66 39413.0 67 26718.0 68 26718.0 69 26718.0 70 19405.5 71 12093.0 72 12093.0 73 12093.0 74 8389.5 75 4686.0 76 4686.0 77 4686.0 78 3106.0 79 1526.0 80 1526.0 81 1526.0 82 987.0 83 448.0 84 448.0 85 448.0 86 292.5 87 137.0 88 137.0 89 137.0 90 83.5 91 30.0 92 30.0 93 30.0 94 22.5 95 15.0 96 15.0 97 15.0 98 10.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.422542343454609 3 0.0 4 0.0 5 8.439754620162815E-4 6 5.425556541533239E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.2056792314518307E-4 14 0.0 15 0.0 16 0.0 17 4.2198773100814076E-4 18 0.0 19 1.808518847177746E-4 20 2.4113584629036614E-4 21 5.425556541533239E-4 22 0.005184420695242872 23 3.0141980786295766E-4 24 0.0027730622323392106 25 0.0012056792314518307 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1658816.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.82513129178928 #Duplication Level Percentage of deduplicated Percentage of total 1 87.70244415798896 64.74644454574351 2 8.985750549173668 13.267484280960268 3 1.8041438524298528 3.9957347032472508 4 0.55495523346348 1.6387857188602788 5 0.26695652375934525 0.9854050207866663 6 0.1435160646952585 0.6357055391165028 7 0.09632278499929933 0.4977729574274844 8 0.06843502706905867 0.4041779886664327 9 0.05054673708298869 0.33584575513708737 >10 0.277226178547508 3.73031703596504 >50 0.02313813400518219 1.2128590048805838 >100 0.021765286879089504 3.320720697364729 >500 0.0023169854719821857 1.1902147768594795 >1k 0.002234235990839965 3.053945845608081 >5k 2.4824844342666275E-4 0.9845861293765967 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5685 0.3427143215401829 No Hit CTGTAGGACGTGGAATATGGCAAGA 5293 0.319083008603727 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5139 0.30979927852154787 No Hit GTATCAACGCAGAGTACTTTTTTTT 4880 0.2941857324742467 No Hit CTTTAGGACGTGAAATATGGCGAGG 4695 0.28303319958331724 No Hit GGTATCAACGCAGAGTACTTTTTTT 3322 0.2002633203441491 No Hit TATCAACGCAGAGTACTTTTTTTTT 2968 0.17892279794745167 No Hit GTCCTACAGTGTGCATTTCTCATTT 2940 0.17723484702341913 No Hit CTGAAGGACCTGGAATATGGCGAGA 2386 0.14383753231220342 No Hit CTGTAGGACCTGGAATATGGCGAGA 2124 0.12804313438018441 No Hit ATTTAGAAATGTCCACTGTAGGACG 2080 0.1253906400709904 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1991 0.12002536749102974 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1963 0.1183374165669972 No Hit GAATATGGCAAGAAAACTGAAAATC 1778 0.10718488367606775 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.0283961572591536E-5 7 0.0 0.0 0.0 0.0 6.0283961572591536E-5 8 0.0 0.0 0.0 0.0 6.0283961572591536E-5 9 0.0 0.0 0.0 0.0 6.0283961572591536E-5 10 0.0 0.0 0.0 6.0283961572591536E-5 6.0283961572591536E-5 11 0.0 0.0 0.0 6.0283961572591536E-5 6.0283961572591536E-5 12 0.0 0.0 0.0 6.0283961572591536E-5 6.0283961572591536E-5 13 0.0 0.0 0.0 6.0283961572591536E-5 6.0283961572591536E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAT 40 1.8838735E-4 17.47847 1 GTCAACG 40 0.0037856072 14.981545 2 CGTTAAA 45 7.064107E-4 14.693005 9 TAGGACC 910 0.0 13.598192 4 GGTATCA 905 0.0 12.470826 1 CGGTAGG 105 1.418739E-7 12.365721 1 CAAGTCG 255 0.0 11.85318 6 CTAGTAC 120 1.08939275E-8 11.8075905 3 GGCGAGG 1510 0.0 11.760673 19 GTGGTAT 300 0.0 11.652313 1 ACACCGT 65 8.4205595E-4 11.625234 6 AGTCGTC 295 0.0 11.526715 8 CCAACGT 75 2.1865497E-4 11.335287 19 ACCTTTT 835 0.0 11.311979 15 AGGACCT 1845 0.0 11.161212 5 ATCCCGT 120 1.3807221E-7 11.0197525 10 TAGGACA 875 0.0 10.903466 4 TCCAACG 165 8.913048E-11 10.8772955 18 TGTAGGA 2880 0.0 10.820005 2 ACGTTAA 70 0.0015647997 10.79486 8 >>END_MODULE