FastQCFastQC Report
Thu 26 May 2016
SRR1511783_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511783_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658816
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT60370.36393427601373507No Hit
GTATCAACGCAGAGTACTTTTTTTT59660.3596541147420811No Hit
CTGTAGGACGTGGAATATGGCAAGA56530.34078523476985995No Hit
GTCCTAAAGTGTGTATTTCTCATTT54760.3301149735715112No Hit
CTTTAGGACGTGAAATATGGCGAGG49960.3011786720166673No Hit
GGTATCAACGCAGAGTACTTTTTTT40580.24463231606157645No Hit
TATCAACGCAGAGTACTTTTTTTTT39300.23691596898028472No Hit
GTCCTACAGTGTGCATTTCTCATTT31920.19242640533971217No Hit
CTGAAGGACCTGGAATATGGCGAGA27790.16752912921023186No Hit
CTGTAGGACCTGGAATATGGCGAGA23640.1425112851576064No Hit
ATTTAGAAATGTCCACTGTAGGACG23220.13997935877155754No Hit
TTTCTAAATTTTCCACCTTTTTCAG22960.13841197577067016No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20020.12068849106832825No Hit
GTCCTTCAGTGTGCATTTCTCATTT19980.1204473552220379No Hit
GAATATGGCAAGAAAACTGAAAATC19340.11658918168139204No Hit
GGAATATGGCGAGAAAACTGAAAAT17890.10784800725336625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTT400.005289424714.24588611
GGTATCA10500.013.7162551
TCCAACG1750.013.02559518
TAGGACC9900.012.8579744
CGGTTTC1600.012.46552613
AATGTCC8250.012.0874188
CCAACGT951.043154E-611.99689719
TAGGACT2700.011.9623944
GCCGGTT1201.0057192E-811.871572511
GGCGAGG13050.011.86283219
TGCCGGT1157.102062E-811.56187910
CCGGTTT1354.7457434E-911.25634812
TGTCCAC9300.011.233314510
GTAATAC1104.971407E-711.2267393
CACCTTT7450.011.218575514
AAATGTC8150.011.1869547
TAAACGT600.005878021511.0828064
CGACTAA600.00588726211.08046717
CCACCTT7550.011.06998413
GAAATGT8550.010.9968246
Produced by FastQC (version 0.11.5)