##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511783_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1658816 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.25252047243335 33.0 31.0 34.0 28.0 34.0 2 31.460935390061344 33.0 31.0 34.0 28.0 34.0 3 31.57622243817277 34.0 31.0 34.0 28.0 34.0 4 35.123244531039006 37.0 35.0 37.0 32.0 37.0 5 34.80424893417956 37.0 35.0 37.0 32.0 37.0 6 34.764982975809254 37.0 35.0 37.0 32.0 37.0 7 34.62636603456924 37.0 35.0 37.0 31.0 37.0 8 34.54928394710444 37.0 35.0 37.0 31.0 37.0 9 36.11444789536633 39.0 37.0 39.0 32.0 39.0 10 35.93984685462402 39.0 37.0 39.0 30.0 39.0 11 35.915886994096994 39.0 37.0 39.0 30.0 39.0 12 35.779209990740384 39.0 35.0 39.0 30.0 39.0 13 35.77485809155446 39.0 35.0 39.0 30.0 39.0 14 36.87197615648752 40.0 37.0 41.0 31.0 41.0 15 36.83770593001273 40.0 37.0 41.0 31.0 41.0 16 36.755090980554805 40.0 37.0 41.0 30.0 41.0 17 36.71154064103553 40.0 37.0 41.0 30.0 41.0 18 36.65482910702573 40.0 37.0 41.0 30.0 41.0 19 36.675287675064624 40.0 37.0 41.0 30.0 41.0 20 36.56354954377098 40.0 37.0 41.0 30.0 41.0 21 36.45609941066399 40.0 36.0 41.0 30.0 41.0 22 36.544402754735906 40.0 37.0 41.0 30.0 41.0 23 36.448182317990664 40.0 37.0 41.0 30.0 41.0 24 36.35853102453798 40.0 37.0 41.0 30.0 41.0 25 35.965271012577645 39.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4239957179611977 1101 2 0.33439326827882 1101 3 0.43027616738230634 1101 4 0.41728398972255576 1101 5 0.45430651462633875 1101 6 0.5727417003656612 1101 7 0.5421268077416315 1101 8 0.4944116895362214 1101 9 0.42693523368111386 1101 10 0.5621661684938459 1101 11 0.3287458647733388 1101 12 0.6101396553767913 1101 13 0.6163312849712526 1101 14 0.5526952365141398 1101 15 0.3740758013369856 1101 16 0.5269869640555171 1101 17 0.6927549860322983 1101 18 0.6908650034732133 1101 19 0.5912610389917461 1101 20 0.48705953565868754 1101 21 0.66213496360308 1101 22 0.5080468181971938 1101 23 0.48422477403878617 1101 24 0.4422699952682194 1101 25 0.59937672905847 1102 1 0.5146073768829531 1102 2 0.5147854251415644 1102 3 0.45404737143232765 1102 4 0.3756503805948981 1102 5 0.43015373212605823 1102 6 0.47358419535980545 1102 7 0.5529923485629737 1102 8 0.4144048891281926 1102 9 0.5001869621181498 1102 10 0.5865758552972764 1102 11 0.5938484375943744 1102 12 0.6627861474996521 1102 13 0.7529892622276648 1102 14 0.9122705888130582 1102 15 0.8039371485733753 1102 16 0.7509017322749401 1102 17 0.910077136472438 1102 18 0.7228722572417752 1102 19 0.885488248179854 1102 20 0.9854009917016029 1102 21 0.9607711039937286 1102 22 0.8868257671563526 1102 23 0.9492933448197007 1102 24 0.9899673015510473 1102 25 0.8435567131908499 1103 1 0.2720763004051463 1103 2 0.21858289349599502 1103 3 0.23418118930668896 1103 4 0.21003653377815112 1103 5 0.3074172948033933 1103 6 0.2047761175080467 1103 7 0.4583218933708224 1103 8 0.1077607422374669 1103 9 0.34485944978105465 1103 10 0.3533591857851519 1103 11 0.33862010983001056 1103 12 0.23138975879368218 1103 13 0.0981133648918302 1103 14 0.29732583136811996 1103 15 0.44089966168281336 1103 16 0.15895519438561934 1103 17 0.3493778039358375 1103 18 0.4397108709341211 1103 19 0.461762404094749 1103 20 0.29664421341163916 1103 21 0.28273560892449723 1103 22 0.40542002908492947 1103 23 0.3678063079181584 1103 24 0.3067152670478208 1103 25 0.3029963203549073 1104 1 0.38980366165149505 1104 2 0.43909311744925716 1104 3 0.37029024480814954 1104 4 0.34518745880249924 1104 5 0.39476101437495714 1104 6 0.490875435911164 1104 7 0.38589151362398155 1104 8 0.407934062588005 1104 9 0.44318661628543765 1104 10 0.3238655148990759 1104 11 0.6232335470257269 1104 12 0.5425660002737231 1104 13 0.5803373181537594 1104 14 0.5024066492843744 1104 15 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0.900490388330141 1107 15 0.8858062319058391 1107 16 0.9965682142771257 1107 17 0.969088473013592 1107 18 0.9762388998672122 1107 19 0.9838540255534625 1107 20 1.0127081073236397 1107 21 0.8657374172427623 1107 22 0.9024363039493011 1107 23 1.0106333121375712 1107 24 0.9056604147271443 1107 25 0.8407600334350747 1108 1 0.17485907738388562 1108 2 0.2084201663670271 1108 3 0.14501334918740838 1108 4 0.08718169300374967 1108 5 0.04232757274223076 1108 6 0.21729579009594602 1108 7 0.3747124535407025 1108 8 0.37675335513365127 1108 9 0.24216854096396645 1108 10 0.08610227873013088 1108 11 0.3558344713839361 1108 12 0.20045315535540453 1108 13 0.40647487363813184 1108 14 0.4736347644173122 1108 15 0.36425827131089505 1108 16 0.4604905975136546 1108 17 0.2947546516530579 1108 18 0.29416835931882446 1108 19 0.3883367758897194 1108 20 0.35937021285440807 1108 21 0.5714139875267463 1108 22 0.4970212459615624 1108 23 0.490303088767412 1108 24 0.4230237482863828 1108 25 0.3492703886837347 1109 1 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3 -0.7004181873764992 2315 4 -0.7632045151409699 2315 5 -0.7249359577590226 2315 6 -0.7070146280238561 2315 7 -0.783066144504609 2315 8 -0.8178094735413097 2315 9 -0.9724514873777608 2315 10 -0.9488161369566512 2315 11 -1.0186843427165257 2315 12 -0.9133596903451178 2315 13 -1.0366785650743608 2315 14 -1.261327155854552 2315 15 -1.1769561814160383 2315 16 -1.3646256349830566 2315 17 -1.2622713388381257 2315 18 -1.2945966981964787 2315 19 -1.1656109309148732 2315 20 -1.3025581131084394 2315 21 -1.346267812039322 2315 22 -1.3412953162868462 2315 23 -1.2242067010081072 2315 24 -1.1209353182215196 2315 25 -1.3924125079815255 2316 1 -1.4812033313554984 2316 2 -1.2525305921744518 2316 3 -1.0505892090713047 2316 4 -0.838445574830537 2316 5 -0.9556395850256436 2316 6 -0.8923949603592334 2316 7 -1.0135327288002571 2316 8 -0.8662474225934815 2316 9 -1.1531746890758683 2316 10 -1.318147472113914 2316 11 -1.1475613247691498 2316 12 -1.1214090247013004 2316 13 -1.1245714397550017 2316 14 -1.4029303470526173 2316 15 -1.3872772247219203 2316 16 -1.4527303774265334 2316 17 -1.4230289032810006 2316 18 -1.5129865371430284 2316 19 -1.4092291570866848 2316 20 -1.4829217902049976 2316 21 -1.3597201420755525 2316 22 -1.4402194580102545 2316 23 -1.48920914498337 2316 24 -1.3828827931198617 2316 25 -1.35366842839845 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19185.0 3 0.0 4 322.0 5 3270.0 6 4177.0 7 3310.0 8 2815.0 9 2438.0 10 2460.0 11 2196.0 12 2121.0 13 2107.0 14 1973.0 15 1949.0 16 2156.0 17 2277.0 18 2437.0 19 2829.0 20 3460.0 21 4227.0 22 5710.0 23 7234.0 24 8532.0 25 10357.0 26 12012.0 27 13543.0 28 16006.0 29 19536.0 30 25557.0 31 32864.0 32 42501.0 33 55666.0 34 73844.0 35 100028.0 36 149100.0 37 273605.0 38 747012.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.096314179789374 20.418514096972835 13.469673891723433 25.015497831514356 2 13.892559512326866 21.08690777036151 42.84097814344689 22.17955457386473 3 18.28382412515915 25.84928045063467 30.64589442108106 25.22100100312512 4 12.074515799220649 17.940326112118523 39.52976098614916 30.45539710251167 5 12.134873197582143 38.79769261569484 36.75117347111066 12.316260715612351 6 30.16573266715537 38.70025367491029 18.650531463405223 12.48348219452911 7 26.647862089586788 32.03869506925422 23.554872873181836 17.75856996797716 8 23.51984789150816 39.31442667541186 21.141404471623133 16.024320961456848 9 26.096926964774873 15.040546992553724 21.256546838226782 37.605979204444616 10 14.690658879586405 29.376314190362287 35.76653468498013 20.166492245071183 11 33.10463607778078 22.044397932018985 26.79308615301516 18.057879837185077 12 23.467099425132144 24.7402364095837 33.2405161271654 18.552148038118755 13 29.05180562521702 22.80566379875767 26.278321405146805 21.864209170878507 14 19.52362407886107 23.744948204020215 28.526370616150317 28.2050571009684 15 21.485927312010496 33.88091265095104 23.635894517535398 20.997265519503067 16 20.187362665517252 28.64647353683202 30.39681941627005 20.769344381380684 17 20.327209286623713 29.894876827809714 29.424059087927773 20.3538547976388 18 20.12571640082396 27.502135563200913 32.82527928102806 19.54686875494706 19 23.01316119449053 26.7802456692002 28.703906882981595 21.502686253327674 20 23.008097341718432 28.131148964080406 29.507371522821096 19.35338217138007 21 21.558991473436475 27.19656670782052 28.28921351132374 22.95522830741927 22 22.375296597090937 29.733255526833595 28.340937150353025 19.550510725722443 23 20.55122448782746 29.289565569659327 29.617992592306802 20.541217350206413 24 22.61802704478728 27.20925045064598 30.386138792931945 19.786583711634805 25 21.483012250325988 28.451489106969863 29.866778232386654 20.1987204103175 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 259.0 1 259.0 2 415.5 3 572.0 4 572.0 5 572.0 6 1441.5 7 2311.0 8 2311.0 9 2311.0 10 3277.5 11 4244.0 12 4244.0 13 4244.0 14 6652.0 15 9060.0 16 9060.0 17 9060.0 18 16100.5 19 23141.0 20 23141.0 21 23141.0 22 37696.5 23 52252.0 24 52252.0 25 52252.0 26 76646.5 27 101041.0 28 101041.0 29 101041.0 30 130724.0 31 160407.0 32 160407.0 33 160407.0 34 175360.0 35 190313.0 36 190313.0 37 190313.0 38 196158.5 39 202004.0 40 202004.0 41 202004.0 42 206724.5 43 211445.0 44 211445.0 45 211445.0 46 211154.0 47 210863.0 48 210863.0 49 210863.0 50 195768.0 51 180673.0 52 180673.0 53 180673.0 54 153931.0 55 127189.0 56 127189.0 57 127189.0 58 106272.0 59 85355.0 60 85355.0 61 85355.0 62 68912.0 63 52469.0 64 52469.0 65 52469.0 66 39691.5 67 26914.0 68 26914.0 69 26914.0 70 19342.0 71 11770.0 72 11770.0 73 11770.0 74 8111.0 75 4452.0 76 4452.0 77 4452.0 78 2994.0 79 1536.0 80 1536.0 81 1536.0 82 972.0 83 408.0 84 408.0 85 408.0 86 259.0 87 110.0 88 110.0 89 110.0 90 67.0 91 24.0 92 24.0 93 24.0 94 12.0 95 0.0 96 0.0 97 0.0 98 2.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4202997800841082 2 0.0 3 0.0 4 0.0 5 0.02941857324742467 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 6.0283961572591536E-5 17 0.0 18 1.808518847177746E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005184420695242872 25 1.808518847177746E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1658816.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.50998828953203 #Duplication Level Percentage of deduplicated Percentage of total 1 86.545085493529 61.88838050158808 2 9.76604679575542 13.967397839989836 3 1.9562717019400309 4.1967889949062345 4 0.6581834051495481 1.88266750378434 5 0.29432572105869736 1.052361443310776 6 0.18194770104297092 0.7806646782534076 7 0.10656802077578724 0.5334474542400606 8 0.07566968697031595 0.4328910743295878 9 0.05716475633530438 0.36790659505004114 >10 0.30492234925009726 4.018811981890515 >50 0.024834331390546877 1.2653047071865515 >100 0.022764473430831406 3.293665430829723 >500 0.0035277521837725396 1.7111080976822006 >1k 0.002351834789181693 3.219199298068493 >5k 3.3597639845452755E-4 1.3894043988902227 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6037 0.36393427601373507 No Hit GTATCAACGCAGAGTACTTTTTTTT 5966 0.3596541147420811 No Hit CTGTAGGACGTGGAATATGGCAAGA 5653 0.34078523476985995 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5476 0.3301149735715112 No Hit CTTTAGGACGTGAAATATGGCGAGG 4996 0.3011786720166673 No Hit GGTATCAACGCAGAGTACTTTTTTT 4058 0.24463231606157645 No Hit TATCAACGCAGAGTACTTTTTTTTT 3930 0.23691596898028472 No Hit GTCCTACAGTGTGCATTTCTCATTT 3192 0.19242640533971217 No Hit CTGAAGGACCTGGAATATGGCGAGA 2779 0.16752912921023186 No Hit CTGTAGGACCTGGAATATGGCGAGA 2364 0.1425112851576064 No Hit ATTTAGAAATGTCCACTGTAGGACG 2322 0.13997935877155754 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2296 0.13841197577067016 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2002 0.12068849106832825 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1998 0.1204473552220379 No Hit GAATATGGCAAGAAAACTGAAAATC 1934 0.11658918168139204 No Hit GGAATATGGCGAGAAAACTGAAAAT 1789 0.10784800725336625 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 6.0283961572591536E-5 9 0.0 0.0 0.0 6.0283961572591536E-5 6.0283961572591536E-5 10 0.0 0.0 0.0 6.0283961572591536E-5 6.0283961572591536E-5 11 0.0 0.0 0.0 1.2056792314518307E-4 6.0283961572591536E-5 12 0.0 0.0 0.0 1.2056792314518307E-4 1.2056792314518307E-4 13 0.0 0.0 0.0 1.2056792314518307E-4 1.2056792314518307E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCGTT 40 0.0052894247 14.245886 11 GGTATCA 1050 0.0 13.716255 1 TCCAACG 175 0.0 13.025595 18 TAGGACC 990 0.0 12.857974 4 CGGTTTC 160 0.0 12.465526 13 AATGTCC 825 0.0 12.087418 8 CCAACGT 95 1.043154E-6 11.996897 19 TAGGACT 270 0.0 11.962394 4 GCCGGTT 120 1.0057192E-8 11.8715725 11 GGCGAGG 1305 0.0 11.862832 19 TGCCGGT 115 7.102062E-8 11.561879 10 CCGGTTT 135 4.7457434E-9 11.256348 12 TGTCCAC 930 0.0 11.2333145 10 GTAATAC 110 4.971407E-7 11.226739 3 CACCTTT 745 0.0 11.2185755 14 AAATGTC 815 0.0 11.186954 7 TAAACGT 60 0.0058780215 11.082806 4 CGACTAA 60 0.005887262 11.080467 17 CCACCTT 755 0.0 11.069984 13 GAAATGT 855 0.0 10.996824 6 >>END_MODULE