FastQCFastQC Report
Thu 26 May 2016
SRR1511782_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511782_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810752
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT83020.4584835471671438No Hit
GTCCTAAAGTGTGTATTTCTCATTT75670.4178926766337963No Hit
CTGTAGGACGTGGAATATGGCAAGA73930.408283409323861No Hit
CTTTAGGACGTGAAATATGGCGAGG64060.3537756688933658No Hit
GTATCAACGCAGAGTACTTTTTTTT52180.2881675679496695No Hit
GTCCTACAGTGTGCATTTCTCATTT43350.23940329763545753No Hit
CTGAAGGACCTGGAATATGGCGAGA34950.19301373131163185No Hit
GGTATCAACGCAGAGTACTTTTTTT33560.1853373625985226No Hit
TATCAACGCAGAGTACTTTTTTTTT32940.18191337079843073No Hit
CTGTAGGACCTGGAATATGGCGAGA32320.1784893789983388No Hit
ATTTAGAAATGTCCACTGTAGGACG29950.16540089421411658No Hit
GTCCTTCAGTGTGCATTTCTCATTT28610.15800065387198248No Hit
TTTCTAAATTTTCCACCTTTTTCAG27540.1520915067331142No Hit
GAATATGGCAAGAAAACTGAAAATC25360.14005230975859753No Hit
GGAATATGGCGAGAAAACTGAAAAT21860.12072332379033683No Hit
ATTCCAGGTCCTTCAGTGTGCATTT18880.10426607288021772No Hit
GCCATATTCCACGTCCTACAGTGGA18140.10017937298978545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG1003.092282E-1014.9858281
TAGGACC13650.013.9086664
GGTATCA9800.013.7624951
ACCTTTT11450.012.37393515
CACCTTT11600.012.29535314
CGAGTAA550.0032040312.02146410
CGTTAGG1001.0519052E-611.9886631
CCACCTT11700.011.86734413
GGCGAGG18750.011.7375319
CTAGGAC3300.011.735243
TTCCACC11050.011.71063211
TAGAACG1052.9316652E-711.6943514
TATGTCG2350.011.65607116
AATGTCC10950.011.55888
TAGAAAT11000.011.4203924
TTTCCAC9450.011.39449510
TCCACCT11750.011.33452412
CCAACGA1002.0683874E-611.33452419
TCCAACG1355.175025E-911.194591518
GTCGTTC600.00613363911.0196768
Produced by FastQC (version 0.11.5)