FastQCFastQC Report
Thu 26 May 2016
SRR1511782_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511782_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810752
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT78650.4343499275439155No Hit
CTGTAGGACGTGGAATATGGCAAGA77250.4266183331566112No Hit
GTCCTAAAGTGTGTATTTCTCATTT73080.4035892270172834No Hit
CTTTAGGACGTGAAATATGGCGAGG69250.38243779380058673No Hit
GTATCAACGCAGAGTACTTTTTTTT64730.3574757890644329No Hit
TATCAACGCAGAGTACTTTTTTTTT43950.24271683808715938No Hit
GGTATCAACGCAGAGTACTTTTTTT43360.23945852330965253No Hit
GTCCTACAGTGTGCATTTCTCATTT41960.23172692892234828No Hit
CTGAAGGACCTGGAATATGGCGAGA39570.21852799278973598No Hit
CTGTAGGACCTGGAATATGGCGAGA33710.18616574771144806No Hit
ATTTAGAAATGTCCACTGTAGGACG31660.17484448450146678No Hit
TTTCTAAATTTTCCACCTTTTTCAG31640.17473403315307673No Hit
GAATATGGCAAGAAAACTGAAAATC28480.1572827201074471No Hit
GTCCTTCAGTGTGCATTTCTCATTT26730.1476182271233167No Hit
GGAATATGGCGAGAAAACTGAAAAT26200.14469126639098012No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21890.12088900081292193No Hit
ACAGTGGACATTTCTAAATTTTCCA21080.11641572120312445No Hit
ATTCCAGGTCCTTCAGTGTGCATTT19980.11034089704167109No Hit
GCCATATTCCACGTCCTACAGTGGA19840.10956773760294065No Hit
TTCCAGGTCCTTCAGTGTGCATTTC19000.10492878097055809No Hit
ACCTGGAATATGGCGAGAAAACTGA18300.10106298377690595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10800.014.6578521
TCCGTAG400.00528989314.2457968
GGACCGA400.00528989314.2457966
TAGGACC12700.013.4654284
CATTTCG658.0444326E-411.68885814
AAATGTC10350.011.5618067
GGCGAGT1001.936187E-611.39695119
ACGTTTC752.0798668E-411.39663713
AGGACCT26400.011.371945
TAGAAAT10950.011.3660244
CACCTTT9900.011.22396114
GTCGAGG1451.2223609E-911.13495319
ACGGCGA600.005889212711.08006417
GGACCTG25250.011.0581236
CCACCTT9800.011.04776113
AGGACGG1553.1286618E-1011.0329645
GAAATGT10950.011.0150156
AATGTCC10800.010.9921268
ACCTTTT9650.010.92423715
GGCGAGG20450.010.91395119
Produced by FastQC (version 0.11.5)