##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511782_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1810752 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.40633780882197 31.0 31.0 34.0 27.0 34.0 2 30.624878503516772 31.0 31.0 34.0 27.0 34.0 3 30.72488667691655 31.0 31.0 34.0 27.0 34.0 4 34.313324519315735 37.0 35.0 37.0 30.0 37.0 5 33.9024217562648 37.0 35.0 37.0 30.0 37.0 6 33.8384842319655 37.0 35.0 37.0 29.0 37.0 7 33.6465490580709 37.0 35.0 37.0 28.0 37.0 8 33.54550761230693 37.0 35.0 37.0 28.0 37.0 9 34.97764022903191 38.0 35.0 39.0 28.0 39.0 10 34.73106366857527 38.0 35.0 39.0 27.0 39.0 11 34.709638868271306 38.0 35.0 39.0 27.0 39.0 12 34.535060019262716 38.0 35.0 39.0 27.0 39.0 13 34.52566226628495 38.0 35.0 39.0 27.0 39.0 14 35.42295355741703 39.0 35.0 41.0 27.0 41.0 15 35.38502180309617 39.0 35.0 41.0 27.0 41.0 16 35.268004122044324 39.0 35.0 40.0 27.0 41.0 17 35.21053849450394 39.0 35.0 40.0 27.0 41.0 18 35.1196591250486 39.0 35.0 40.0 26.0 41.0 19 35.14331835613049 39.0 35.0 40.0 26.0 41.0 20 35.012961465733575 39.0 34.0 40.0 25.0 41.0 21 34.85529713621744 39.0 34.0 40.0 25.0 41.0 22 34.96003980666596 39.0 35.0 40.0 25.0 41.0 23 34.8262964779274 39.0 34.0 40.0 25.0 41.0 24 34.69808400045948 39.0 34.0 41.0 24.0 41.0 25 34.23907746615771 38.0 34.0 40.0 21.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34322685159737176 1101 2 0.2372034968107144 1101 3 0.32113028469044025 1101 4 0.30635132391540054 1101 5 0.40693430105933714 1101 6 0.3886937054205575 1101 7 0.2918276785066283 1101 8 0.34191619311647514 1101 9 0.3942679620211962 1101 10 0.3897519636282283 1101 11 0.16141045019749356 1101 12 0.3153649544863555 1101 13 0.3981392607160146 1101 14 0.5808070857140919 1101 15 0.3728270759129586 1101 16 0.27742097136709276 1101 17 0.4356865240037493 1101 18 0.42643967669968674 1101 19 0.49067797246760847 1101 20 0.4496595969642243 1101 21 0.3528985130999658 1101 22 0.1618880296400036 1101 23 0.06947089978667975 1101 24 0.11237987551342599 1101 25 0.16241111906544603 1102 1 0.6264959141318514 1102 2 0.5045368854821781 1102 3 0.6201324561997055 1102 4 0.5549913551780321 1102 5 0.725523096727386 1102 6 0.5955804333895784 1102 7 0.8782537131161874 1102 8 0.7651634005050028 1102 9 0.7428343115867051 1102 10 0.8769177870715765 1102 11 1.0490228045377918 1102 12 0.9996971240109644 1102 13 1.1745609126822103 1102 14 1.2811254849254183 1102 15 1.1854193701806466 1102 16 1.262329033210527 1102 17 1.2237196189390431 1102 18 1.1705742617678823 1102 19 1.2726860429533602 1102 20 1.1855757863395056 1102 21 1.4368748106168923 1102 22 0.9561370997373615 1102 23 1.2046438502347954 1102 24 1.3453879094486823 1102 25 1.3091996116035105 1103 1 0.3235630451207392 1103 2 0.3812855697213209 1103 3 0.3994431361544706 1103 4 0.3469363297327419 1103 5 0.47919954732654446 1103 6 0.5770996342551555 1103 7 0.5378797678996818 1103 8 0.3833980381814328 1103 9 0.6727880560681214 1103 10 0.6597248160618108 1103 11 0.6983232628792848 1103 12 0.6688892903405304 1103 13 0.5453754203049215 1103 14 0.6593017289175052 1103 15 0.7945717918330715 1103 16 0.6627043240915427 1103 17 0.8157797225688057 1103 18 0.8227305192437981 1103 19 0.9460874886591952 1103 20 0.8566913014435755 1103 21 0.8278657905868769 1103 22 0.884998001522888 1103 23 1.0460046160946064 1103 24 1.0251289301963808 1103 25 0.8459026112120398 1104 1 0.4205849968477473 1104 2 0.5027093108782097 1104 3 0.45226642995388744 1104 4 0.5171493447140136 1104 5 0.5382910475346208 1104 6 0.4496482713754233 1104 7 0.517786395032438 1104 8 0.6388898713066951 1104 9 0.7216865683444453 1104 10 0.5863373836856169 1104 11 0.7351873390340913 1104 12 0.8861697730704563 1104 13 0.9890799042832796 1104 14 0.917633761793077 1104 15 0.9340796986162587 1104 16 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-1.3869691366008325 2315 12 -1.3806631864767525 2315 13 -1.5049643124716567 2315 14 -1.804280265533393 2315 15 -1.640517758892372 2315 16 -1.670580039789506 2315 17 -1.8299899504765662 2315 18 -1.8507426132341251 2315 19 -1.7587099621639553 2315 20 -1.6408371814943195 2315 21 -1.9849366677948481 2315 22 -1.8158306192208045 2315 23 -1.8859753744110748 2315 24 -1.5647504666843233 2315 25 -1.6910120021942987 2316 1 -1.9805282056881381 2316 2 -1.746063741306049 2316 3 -1.3895762435798353 2316 4 -1.3272725174752082 2316 5 -1.5374829400169148 2316 6 -1.466601714189899 2316 7 -1.5737993411939897 2316 8 -1.5017801288087824 2316 9 -2.022359992159963 2316 10 -2.0627162786814424 2316 11 -2.0283079321646795 2316 12 -2.0662131039345226 2316 13 -1.9951308651429116 2316 14 -2.1140087893345623 2316 15 -2.142709335645897 2316 16 -2.2780371303639413 2316 17 -2.2777519996724465 2316 18 -2.492570216358196 2316 19 -2.4202438510309605 2316 20 -2.2966217623910765 2316 21 -2.3556909354608564 2316 22 -2.3118915106768796 2316 23 -2.1924219045936013 2316 24 -2.1964420273233145 2316 25 -2.1577533925563728 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38334.0 3 1.0 4 717.0 5 5817.0 6 7277.0 7 5837.0 8 4596.0 9 4360.0 10 3935.0 11 3759.0 12 3547.0 13 3286.0 14 3344.0 15 3264.0 16 3379.0 17 3516.0 18 3952.0 19 4236.0 20 5294.0 21 6857.0 22 8627.0 23 10455.0 24 12965.0 25 15195.0 26 17547.0 27 20112.0 28 23283.0 29 28228.0 30 35483.0 31 45536.0 32 58318.0 33 75368.0 34 97986.0 35 132542.0 36 197265.0 37 347222.0 38 569312.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.12916653268416 21.34175076148267 13.962049499977269 24.567033205855903 2 13.92338652670272 21.841367564415226 43.01919865337716 21.216047255504893 3 18.51131463612908 26.55830284876118 31.004908457922454 23.92547405718729 4 12.232431608525077 18.69466387445658 39.72769324567914 29.3452112713392 5 11.691915230231103 39.50879096354794 37.012309779546456 11.786984026674503 6 29.720580178842827 39.50634874350546 18.631485703177464 12.141585374474252 7 26.481649612978476 32.2446696179267 23.828276870604036 17.445403898490795 8 23.049650090128303 40.50422145053547 21.051640423426292 15.394488035909943 9 26.0457533665571 14.87464876471212 21.574903686424204 37.50469418230657 10 14.598796522107943 29.835394355494294 36.00669776976637 19.55911135263139 11 32.77986162655074 21.78321492948786 27.70705209769201 17.729871346269395 12 23.798952037606476 24.846859205457182 33.631828102357474 17.72236065457887 13 29.1696488530732 23.280935213657088 26.433382373732016 21.116033559537694 14 19.064344537518114 24.72762697487011 28.645391527939772 27.562636959672005 15 20.91937493372919 35.67545417594458 23.330638320432616 20.074532569893613 16 19.47411904004524 29.07907874739335 31.531540487046268 19.915261725515148 17 19.734508093874812 31.002423302583676 29.712682907432935 19.550385696108577 18 19.504580701135275 27.572788939825454 34.1949657296511 18.727664629388173 19 23.08985438094228 26.876623634821335 28.9662526950129 21.067269289223482 20 23.11465070865585 28.515390290884675 29.99726080655993 18.372698193899552 21 21.032421888806418 27.42275032693599 28.528865355388263 23.015962428869333 22 22.30830063973421 30.570889884423707 28.630867175626477 18.4899423002156 23 19.754720690630194 30.35473659562436 30.184586293429472 19.705956420315978 24 22.589255388183965 27.531334999378686 31.05018846562746 18.82922114680989 25 20.980777004862077 29.19771276842498 30.465089565196262 19.356420661516676 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 245.0 1 245.0 2 442.5 3 640.0 4 640.0 5 640.0 6 1594.0 7 2548.0 8 2548.0 9 2548.0 10 3669.0 11 4790.0 12 4790.0 13 4790.0 14 7576.0 15 10362.0 16 10362.0 17 10362.0 18 18624.5 19 26887.0 20 26887.0 21 26887.0 22 44274.0 23 61661.0 24 61661.0 25 61661.0 26 90993.0 27 120325.0 28 120325.0 29 120325.0 30 155135.0 31 189945.0 32 189945.0 33 189945.0 34 207549.5 35 225154.0 36 225154.0 37 225154.0 38 229168.5 39 233183.0 40 233183.0 41 233183.0 42 234199.0 43 235215.0 44 235215.0 45 235215.0 46 231682.0 47 228149.0 48 228149.0 49 228149.0 50 208055.5 51 187962.0 52 187962.0 53 187962.0 54 155196.0 55 122430.0 56 122430.0 57 122430.0 58 100352.5 59 78275.0 60 78275.0 61 78275.0 62 61767.0 63 45259.0 64 45259.0 65 45259.0 66 33957.0 67 22655.0 68 22655.0 69 22655.0 70 16213.5 71 9772.0 72 9772.0 73 9772.0 74 6684.5 75 3597.0 76 3597.0 77 3597.0 78 2403.5 79 1210.0 80 1210.0 81 1210.0 82 795.5 83 381.0 84 381.0 85 381.0 86 232.5 87 84.0 88 84.0 89 84.0 90 50.5 91 17.0 92 17.0 93 17.0 94 10.5 95 4.0 96 4.0 97 4.0 98 3.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38845739228784504 2 0.0 3 0.0 4 0.0 5 0.0267292263103948 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.6567702258509174E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004252376913017355 25 2.209026967801223E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1810752.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.73279355851489 #Duplication Level Percentage of deduplicated Percentage of total 1 87.73239229345026 61.178248001938094 2 8.78119764318592 12.246748848976022 3 1.7251337721534952 3.6089519158320518 4 0.59684525778874 1.664787485910432 5 0.2956541716412469 1.0308395657886398 6 0.17318774826999944 0.7246119298185548 7 0.11980022326159988 0.5847802965875597 8 0.08571787857685029 0.4781877704858678 9 0.056985326560083975 0.3576371411591002 >10 0.3696504327476873 4.755664397893039 >50 0.02954146630320085 1.4131662018965847 >100 0.02646814096328829 3.761411789497305 >500 0.004028807446587888 1.9181591507769404 >1k 0.0030018565288301904 4.277394098350956 >5k 3.9498112221449875E-4 1.9994114050888783 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 7865 0.4343499275439155 No Hit CTGTAGGACGTGGAATATGGCAAGA 7725 0.4266183331566112 No Hit GTCCTAAAGTGTGTATTTCTCATTT 7308 0.4035892270172834 No Hit CTTTAGGACGTGAAATATGGCGAGG 6925 0.38243779380058673 No Hit GTATCAACGCAGAGTACTTTTTTTT 6473 0.3574757890644329 No Hit TATCAACGCAGAGTACTTTTTTTTT 4395 0.24271683808715938 No Hit GGTATCAACGCAGAGTACTTTTTTT 4336 0.23945852330965253 No Hit GTCCTACAGTGTGCATTTCTCATTT 4196 0.23172692892234828 No Hit CTGAAGGACCTGGAATATGGCGAGA 3957 0.21852799278973598 No Hit CTGTAGGACCTGGAATATGGCGAGA 3371 0.18616574771144806 No Hit ATTTAGAAATGTCCACTGTAGGACG 3166 0.17484448450146678 No Hit TTTCTAAATTTTCCACCTTTTTCAG 3164 0.17473403315307673 No Hit GAATATGGCAAGAAAACTGAAAATC 2848 0.1572827201074471 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2673 0.1476182271233167 No Hit GGAATATGGCGAGAAAACTGAAAAT 2620 0.14469126639098012 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2189 0.12088900081292193 No Hit ACAGTGGACATTTCTAAATTTTCCA 2108 0.11641572120312445 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1998 0.11034089704167109 No Hit GCCATATTCCACGTCCTACAGTGGA 1984 0.10956773760294065 No Hit TTCCAGGTCCTTCAGTGTGCATTTC 1900 0.10492878097055809 No Hit ACCTGGAATATGGCGAGAAAACTGA 1830 0.10106298377690595 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 5.5225674195030574E-5 0.0 0.0 0.0 11 0.0 5.5225674195030574E-5 0.0 0.0 0.0 12 0.0 5.5225674195030574E-5 0.0 0.0 0.0 13 0.0 5.5225674195030574E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1080 0.0 14.657852 1 TCCGTAG 40 0.005289893 14.245796 8 GGACCGA 40 0.005289893 14.245796 6 TAGGACC 1270 0.0 13.465428 4 CATTTCG 65 8.0444326E-4 11.688858 14 AAATGTC 1035 0.0 11.561806 7 GGCGAGT 100 1.936187E-6 11.396951 19 ACGTTTC 75 2.0798668E-4 11.396637 13 AGGACCT 2640 0.0 11.37194 5 TAGAAAT 1095 0.0 11.366024 4 CACCTTT 990 0.0 11.223961 14 GTCGAGG 145 1.2223609E-9 11.134953 19 ACGGCGA 60 0.0058892127 11.080064 17 GGACCTG 2525 0.0 11.058123 6 CCACCTT 980 0.0 11.047761 13 AGGACGG 155 3.1286618E-10 11.032964 5 GAAATGT 1095 0.0 11.015015 6 AATGTCC 1080 0.0 10.992126 8 ACCTTTT 965 0.0 10.924237 15 GGCGAGG 2045 0.0 10.913951 19 >>END_MODULE