FastQCFastQC Report
Thu 26 May 2016
SRR1511781_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511781_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961650
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT48430.5036135808246243No Hit
GTCCTAAAGTGTGTATTTCTCATTT47210.4909270524619144No Hit
CTGTAGGACGTGGAATATGGCAAGA47020.48895128165132845No Hit
CTTTAGGACGTGAAATATGGCGAGG39790.41376800291166227No Hit
GTATCAACGCAGAGTACTTTTTTTT30660.31882701606613634No Hit
CTGAAGGACCTGGAATATGGCGAGA21980.2285654864035772No Hit
GTCCTACAGTGTGCATTTCTCATTT20340.21151146467009826No Hit
GGTATCAACGCAGAGTACTTTTTTT19180.1994488639317839No Hit
TATCAACGCAGAGTACTTTTTTTTT19120.19882493630738834No Hit
ATTTAGAAATGTCCACTGTAGGACG18900.1965372016846046No Hit
GTCCTTCAGTGTGCATTTCTCATTT18220.1894660219414548No Hit
TTTCTAAATTTTCCACCTTTTTCAG17900.18613840794467842No Hit
GAATATGGCAAGAAAACTGAAAATC15160.1576457130972807No Hit
CTGTAGGACCTGGAATATGGCGAGA14060.14620703998336193No Hit
GGAATATGGCGAGAAAACTGAAAAT13420.13955181198980918No Hit
ATTCCAGGTCCTTCAGTGTGCATTT12600.13102480112306975No Hit
GCCATATTCCACGTCCTACAGTGGA10440.1085634066448292No Hit
TTCCAGGTCCTTCAGTGTGCATTTC10420.10835543077003067No Hit
ACAGTGGACATTTCTAAATTTTCCA10310.1072115634586388No Hit
CCATATTCCAGGTCCTTCAGTGTGC10110.10513180471065356No Hit
GACCTGGAATATGGCGAGAAAACTG9980.10377996152446316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACT804.656613E-1016.53468719
GACTGAC402.877949E-416.5338297
GTCGAGG704.7127105E-714.84747519
TCCAACG1850.013.78954618
TAGGACC5200.013.6267824
CCGTAGG450.00837872513.2888331
CGTAGGA450.00837872513.2888332
GGTATCA4900.013.2210331
GGCCTAC1453.6379788E-1213.0597141
CTCGAAC957.919334E-812.9293818
CCAACGA1403.6379788E-1212.82281919
CCCTTAG550.002140310812.6847951
GAATACG604.2836322E-412.59720413
CCGTTTC1353.092282E-1112.59720213
AATGTCC5350.012.5383378
AAATGTC5050.012.5348417
GAAATGT5050.012.3477546
ACCTTTT6500.012.20959715
CGGTAGG904.365109E-612.181431
GGCGAGG11300.012.0404319
Produced by FastQC (version 0.11.5)