##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511781_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 961650 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.56490927052462 31.0 31.0 34.0 27.0 34.0 2 27.850097228721467 31.0 28.0 34.0 10.0 34.0 3 29.600258929964124 31.0 30.0 34.0 16.0 34.0 4 34.00852493110799 37.0 35.0 37.0 30.0 37.0 5 34.37110903135236 37.0 35.0 37.0 32.0 37.0 6 34.58553007851089 37.0 35.0 37.0 32.0 37.0 7 34.60821920657204 37.0 35.0 37.0 32.0 37.0 8 34.62649300681121 37.0 35.0 37.0 32.0 37.0 9 36.30791452191546 39.0 37.0 39.0 32.0 39.0 10 36.20249259085946 39.0 37.0 39.0 32.0 39.0 11 36.20032652212343 39.0 37.0 39.0 32.0 39.0 12 36.12432069879894 39.0 37.0 39.0 32.0 39.0 13 36.13291738158374 39.0 37.0 39.0 32.0 39.0 14 37.37696875162481 40.0 38.0 41.0 32.0 41.0 15 37.35870535017938 40.0 38.0 41.0 32.0 41.0 16 37.32963136276192 40.0 38.0 41.0 32.0 41.0 17 37.315005459366716 40.0 38.0 41.0 32.0 41.0 18 37.29589039671398 40.0 38.0 41.0 32.0 41.0 19 37.31752300733115 40.0 38.0 41.0 32.0 41.0 20 37.28767638953882 40.0 38.0 41.0 32.0 41.0 21 37.18955649144699 40.0 38.0 41.0 32.0 41.0 22 37.124877034264024 40.0 38.0 41.0 32.0 41.0 23 37.03991888940883 40.0 38.0 41.0 32.0 41.0 24 36.99343420163261 40.0 38.0 41.0 32.0 41.0 25 36.91400093589144 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.21413042883085254 1101 2 2.7668451381369437 1101 3 1.3183015190142022 1101 4 0.14333447923149123 1101 5 -0.33878676722788725 1101 6 -0.3754518961906541 1101 7 -0.5040168305306736 1101 8 -0.5408255583351007 1101 9 -0.5699959885975119 1101 10 -0.6570017624001494 1101 11 -0.5759545073387144 1101 12 -0.6422341201315618 1101 13 -0.6287567623727099 1101 14 -0.7013147303381757 1101 15 -0.6059133963469563 1101 16 -0.5959244503355237 1101 17 -0.4504693866905285 1101 18 -0.421840751753372 1101 19 -0.3719594445718073 1101 20 -0.4822755289213845 1101 21 -0.39969400990254655 1101 22 -0.17732492834189628 1101 23 -0.37830104258969754 1101 24 -0.28335338356811945 1101 25 -0.2558140693716169 1102 1 1.2801468182633364 1102 2 4.071297575147707 1102 3 2.382638068060192 1102 4 1.4282862435416703 1102 5 1.0418318115392395 1102 6 0.9330940767790139 1102 7 0.843465511078918 1102 8 0.8496503773302635 1102 9 0.9920033081246018 1102 10 0.8145995707701061 1102 11 0.848832980430366 1102 12 0.8625091834398688 1102 13 0.9425819882388353 1102 14 1.0268893884240526 1102 15 0.9003415724641997 1102 16 1.0101252683533204 1102 17 1.2781226815580027 1102 18 1.1238207116761814 1102 19 1.1816540277863155 1102 20 1.2696795071598288 1102 21 1.1780621514232834 1102 22 1.1629774811492979 1102 23 1.309783748261573 1102 24 0.8206725001158333 1102 25 1.149499500833862 1103 1 1.050905526296738 1103 2 3.778394465013161 1103 3 2.106067646572683 1103 4 1.388733404060929 1103 5 0.8697978453452109 1103 6 0.755801530344435 1103 7 0.7375748330901857 1103 8 0.557550264033317 1103 9 0.6528468172017057 1103 10 0.842046422297706 1103 11 0.7874638040647923 1103 12 0.7965108542836461 1103 13 0.7992079519304056 1103 14 0.8900842858655267 1103 15 0.7767449095740986 1103 16 0.8140338220816332 1103 17 0.8189057655999221 1103 18 1.0190305780468236 1103 19 0.8699577398823806 1103 20 0.6975509361127266 1103 21 0.9195391315448234 1103 22 0.9086506442701889 1103 23 0.8651466608703444 1103 24 1.1032797030546888 1103 25 1.1153063314568428 1104 1 1.0976021795583009 1104 2 3.86284284212579 1104 3 2.2890756839632793 1104 4 1.4287152154705822 1104 5 1.150085535148449 1104 6 0.8474686224199175 1104 7 0.9058334740886309 1104 8 0.8624407638035976 1104 9 1.0422528141425431 1104 10 1.1321175695591137 1104 11 0.9895500089076492 1104 12 1.0425701506007385 1104 13 1.0606916266306854 1104 14 1.200444597680736 1104 15 1.219621013509979 1104 16 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0.651283161050614 1107 17 0.8231421541510571 1107 18 0.6085177723802886 1107 19 0.7404670715173012 1107 20 0.8122653900142609 1107 21 0.8748283446766578 1107 22 0.9934903563115682 1107 23 1.0074005353805973 1107 24 0.9237764895754452 1107 25 0.9002386843412538 1108 1 0.9271238498834826 1108 2 3.8343623762442327 1108 3 2.0060901387004364 1108 4 1.0694201636950567 1108 5 0.7936619928935684 1108 6 0.7743575357425456 1108 7 0.613680696038152 1108 8 0.654716255936151 1108 9 0.5044362608964121 1108 10 0.4781987689763696 1108 11 0.6218092831471154 1108 12 0.7628851232894931 1108 13 0.6428501771515798 1108 14 0.6772712704209312 1108 15 0.627959484472882 1108 16 0.7663130243608336 1108 17 0.7399958840584588 1108 18 0.8220745126697082 1108 19 0.6020015245577497 1108 20 0.8101990092871176 1108 21 0.7758144351885434 1108 22 0.737860420848385 1108 23 0.7704922899001829 1108 24 1.016909932474391 1108 25 0.9042551993530807 1109 1 0.5861811316420962 1109 2 2.905505247323692 1109 3 1.5487506593254459 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-1.2112565191601945 2315 13 -1.4233491224187205 2315 14 -1.5896930374700773 2315 15 -1.4964764874768122 2315 16 -1.5838542177179349 2315 17 -1.3285009242672174 2315 18 -1.6065868055621664 2315 19 -1.6283620199808055 2315 20 -1.4951026474029803 2315 21 -1.6422949013902794 2315 22 -1.4859666077897487 2315 23 -1.6256209876305974 2315 24 -1.6634838315486888 2315 25 -1.8454860721834763 2316 1 -1.2239867749113529 2316 2 -1.2511776171904998 2316 3 -0.9287769145876688 2316 4 -0.9898861061375328 2316 5 -1.0012006835480562 2316 6 -1.2576207988673715 2316 7 -1.2109445364818185 2316 8 -1.4534465627529798 2316 9 -1.3909297528906492 2316 10 -1.6816655487826893 2316 11 -1.586227701205658 2316 12 -1.4101370892064082 2316 13 -1.4331701751845571 2316 14 -1.5766131808319415 2316 15 -1.7606224172669158 2316 16 -1.5108234238282492 2316 17 -1.5863800870543514 2316 18 -1.6082789176837267 2316 19 -1.871354422482412 2316 20 -1.9599891951116106 2316 21 -1.8888097031198043 2316 22 -1.8795484860342029 2316 23 -1.833704011664544 2316 24 -1.7274822329704094 2316 25 -1.9354975088206814 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24572.0 3 1.0 4 241.0 5 1124.0 6 1291.0 7 1156.0 8 1007.0 9 955.0 10 953.0 11 907.0 12 826.0 13 846.0 14 802.0 15 852.0 16 889.0 17 932.0 18 1093.0 19 1271.0 20 1755.0 21 2098.0 22 2427.0 23 2946.0 24 3428.0 25 3984.0 26 4461.0 27 5023.0 28 6120.0 29 7471.0 30 9622.0 31 12934.0 32 17902.0 33 25642.0 34 38512.0 35 63200.0 36 111536.0 37 160660.0 38 442211.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.908698590963446 19.867935319502937 13.153746165444808 27.069619924088805 2 14.213108315945824 20.65262940803489 43.652188412569906 21.482073863449376 3 17.31960692559663 25.113190869859096 30.76774294181875 26.799459262725524 4 11.057453335413092 17.122965735974628 39.90630686840326 31.91327406020902 5 11.369972557497709 38.40265710806564 37.60340332783235 12.623967006604294 6 29.051573085702103 38.66863551518492 19.108610765927136 13.171180633185841 7 25.75198876930276 31.550980086309988 24.124681536941715 18.57234960744554 8 22.05750532938179 40.27224042011127 21.605053813757603 16.06520043674934 9 25.170800187178287 14.739354234908749 21.837882805594553 38.25196277231841 10 14.417303592783238 28.604169916289713 36.50995684500598 20.46856964592107 11 32.071335725055896 21.180367077419017 28.248427182446832 18.49987001507825 12 23.517495970467426 23.790880257890084 34.23854832839391 18.453075443248583 13 28.52295533718089 22.795299745229553 26.70857380543857 21.97317111215099 14 18.389018873810638 23.98741745957469 28.78604481880102 28.837518847813655 15 20.197161129309 35.40300525139084 23.594655020017676 20.805178599282485 16 18.879633962460353 27.838194769406748 32.49456663027089 20.78760463786201 17 19.20444820188697 30.099402379459406 30.46783279103455 20.228316627619076 18 18.890760671762074 26.63682212863308 35.06837206884001 19.404045130764832 19 22.43378034374324 26.065358634344378 29.15963013493503 22.341230886977357 20 22.379937752626482 27.859287243655935 30.554351024856313 19.206423978861267 21 20.12518119973462 26.789171899014814 28.8083140781535 24.277332823097066 22 21.59804079595264 30.454911789021594 28.72303364652174 19.22401376850403 23 18.80137014791275 29.777631732193065 30.877618421709396 20.543379698184783 24 22.103257795081692 26.488407207746828 31.809673554956575 19.5986614422149 25 20.282703958437757 28.350987165701998 31.29116656718405 20.075142308676202 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 247.0 1 247.0 2 311.5 3 376.0 4 376.0 5 376.0 6 849.5 7 1323.0 8 1323.0 9 1323.0 10 1882.5 11 2442.0 12 2442.0 13 2442.0 14 3715.5 15 4989.0 16 4989.0 17 4989.0 18 9282.0 19 13575.0 20 13575.0 21 13575.0 22 22481.5 23 31388.0 24 31388.0 25 31388.0 26 46733.5 27 62079.0 28 62079.0 29 62079.0 30 81492.0 31 100905.0 32 100905.0 33 100905.0 34 109117.5 35 117330.0 36 117330.0 37 117330.0 38 119138.0 39 120946.0 40 120946.0 41 120946.0 42 121890.5 43 122835.0 44 122835.0 45 122835.0 46 122007.5 47 121180.0 48 121180.0 49 121180.0 50 110658.5 51 100137.0 52 100137.0 53 100137.0 54 83296.0 55 66455.0 56 66455.0 57 66455.0 58 55607.5 59 44760.0 60 44760.0 61 44760.0 62 35839.5 63 26919.0 64 26919.0 65 26919.0 66 20417.0 67 13915.0 68 13915.0 69 13915.0 70 10085.0 71 6255.0 72 6255.0 73 6255.0 74 4331.5 75 2408.0 76 2408.0 77 2408.0 78 1631.5 79 855.0 80 855.0 81 855.0 82 545.0 83 235.0 84 235.0 85 235.0 86 153.0 87 71.0 88 71.0 89 71.0 90 45.0 91 19.0 92 19.0 93 19.0 94 11.5 95 4.0 96 4.0 97 4.0 98 3.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.207299953205428 3 0.0 4 0.0 5 3.1196381219778506E-4 6 5.199396869963084E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 3.1196381219778506E-4 18 0.0 19 2.0797587479852336E-4 20 3.1196381219778506E-4 21 4.159517495970467E-4 22 0.003639577808974159 23 2.0797587479852336E-4 24 0.0018717828731867104 25 8.319034991940934E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 961650.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.27442124535877 #Duplication Level Percentage of deduplicated Percentage of total 1 89.4149799128641 63.730009439547686 2 7.3215033648933465 10.436718299574398 3 1.5027583470827186 3.2132469437985827 4 0.5717815447866185 1.6301359473377364 5 0.29644541455996026 1.056448767680081 6 0.18694191807424412 0.7994506210343413 7 0.11814619701570764 0.5894561270244286 8 0.0908051183840748 0.5177665807152976 9 0.0650955223679467 0.4175681114195737 >10 0.3604581143846595 4.669138082366891 >50 0.03391682243838423 1.7072106426227962 >100 0.03050418756221037 4.540106539099642 >500 0.003701964214480959 1.9932689078492802 >1k 0.002961571371584767 4.699474989929308 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4843 0.5036135808246243 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4721 0.4909270524619144 No Hit CTGTAGGACGTGGAATATGGCAAGA 4702 0.48895128165132845 No Hit CTTTAGGACGTGAAATATGGCGAGG 3979 0.41376800291166227 No Hit GTATCAACGCAGAGTACTTTTTTTT 3066 0.31882701606613634 No Hit CTGAAGGACCTGGAATATGGCGAGA 2198 0.2285654864035772 No Hit GTCCTACAGTGTGCATTTCTCATTT 2034 0.21151146467009826 No Hit GGTATCAACGCAGAGTACTTTTTTT 1918 0.1994488639317839 No Hit TATCAACGCAGAGTACTTTTTTTTT 1912 0.19882493630738834 No Hit ATTTAGAAATGTCCACTGTAGGACG 1890 0.1965372016846046 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1822 0.1894660219414548 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1790 0.18613840794467842 No Hit GAATATGGCAAGAAAACTGAAAATC 1516 0.1576457130972807 No Hit CTGTAGGACCTGGAATATGGCGAGA 1406 0.14620703998336193 No Hit GGAATATGGCGAGAAAACTGAAAAT 1342 0.13955181198980918 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1260 0.13102480112306975 No Hit GCCATATTCCACGTCCTACAGTGGA 1044 0.1085634066448292 No Hit TTCCAGGTCCTTCAGTGTGCATTTC 1042 0.10835543077003067 No Hit ACAGTGGACATTTCTAAATTTTCCA 1031 0.1072115634586388 No Hit CCATATTCCAGGTCCTTCAGTGTGC 1011 0.10513180471065356 No Hit GACCTGGAATATGGCGAGAAAACTG 998 0.10377996152446316 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGAACT 80 4.656613E-10 16.534687 19 GACTGAC 40 2.877949E-4 16.533829 7 GTCGAGG 70 4.7127105E-7 14.847475 19 TCCAACG 185 0.0 13.789546 18 TAGGACC 520 0.0 13.626782 4 CCGTAGG 45 0.008378725 13.288833 1 CGTAGGA 45 0.008378725 13.288833 2 GGTATCA 490 0.0 13.221033 1 GGCCTAC 145 3.6379788E-12 13.059714 1 CTCGAAC 95 7.919334E-8 12.92938 18 CCAACGA 140 3.6379788E-12 12.822819 19 CCCTTAG 55 0.0021403108 12.684795 1 GAATACG 60 4.2836322E-4 12.597204 13 CCGTTTC 135 3.092282E-11 12.597202 13 AATGTCC 535 0.0 12.538337 8 AAATGTC 505 0.0 12.534841 7 GAAATGT 505 0.0 12.347754 6 ACCTTTT 650 0.0 12.209597 15 CGGTAGG 90 4.365109E-6 12.18143 1 GGCGAGG 1130 0.0 12.04043 19 >>END_MODULE