FastQCFastQC Report
Thu 26 May 2016
SRR1511781_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511781_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961650
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47510.49404669058389233No Hit
CTGTAGGACGTGGAATATGGCAAGA42710.44413248063224664No Hit
GTCCTAAAGTGTGTATTTCTCATTT36920.38392346487807416No Hit
GGTATCAACGCAGAGTACTTTTTTT36660.38121977850569333No Hit
TATCAACGCAGAGTACTTTTTTTTT36450.37903603182030887No Hit
CTTTAGGACGTGAAATATGGCGAGG33550.348879529974523No Hit
GTCCTACAGTGGACATTTCTAAATT32680.3398325794207872No Hit
CTGAAGGACCTGGAATATGGCGAGA25110.2611137108095461No Hit
TTTCTAAATTTTCCACCTTTTTCAG22760.2366765455207196No Hit
ATTTAGAAATGTCCACTGTAGGACG19520.2029844538033588No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17500.18197889044870796No Hit
GGAATATGGCGAGAAAACTGAAAAT17290.17979514376332345No Hit
GAATATGGCAAGAAAACTGAAAATC17240.17927520407632716No Hit
CTGTAGGACCTGGAATATGGCGAGA14370.14943066604273905No Hit
GTCCTACAGTGTGCATTTCTCATTT13820.14371132948577964No Hit
ACAGTGGACATTTCTAAATTTTCCA13690.14235948629958925No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13170.13695211355482764No Hit
GTCCTTCAGTGTGCATTTCTCATTT13050.1357042583060365No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12340.1283211147506889No Hit
ATTCCAGGTCCTTCAGTGTGCATTT12000.12478552487911403No Hit
GAAATATGGCGAGGAAAACTGAAAA11170.11615452607497531No Hit
ACCTGGAATATGGCGAGAAAACTGA10960.1139707793895908No Hit
GCCATATTCCACGTCCTACAGTGGA10850.11282691207819893No Hit
TTCCAGGTCCTTCAGTGTGCATTTC10700.11126709301721No Hit
CCACTGTAGGACGTGGAATATGGCA10100.10502781677325432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5200.013.5180474
GGTATCA9650.013.4279261
CAGGACG604.0925294E-412.6655574
AAATGTC5400.012.4883487
GTGGTAT2550.012.3302291
AATGTCC5400.012.3124568
TAGAAAT6000.012.1905994
ATGTCCA5400.012.1365649
AGAACGT550.00306899512.0898495
TTGCACC550.00306899512.0898494
TAAGACC550.00306899512.0898494
GGACGTA550.003071386412.08859356
AGGACGG1052.7185888E-711.7608755
AATGCCG658.027938E-411.69067618
GTATTAG907.2127295E-611.6452161
CTAAGAC752.0739333E-411.3990023
AGGACCT12650.011.1887445
CCACCTT5800.011.135797513
GCAGTAC600.0058767311.0823623
TGTCCAC6100.011.05525910
Produced by FastQC (version 0.11.5)