FastQCFastQC Report
Thu 26 May 2016
SRR1511780_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511780_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180836
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT50040.4237675680619494No Hit
CTGTAGGACGTGGAATATGGCAAGA45280.3834571439217639No Hit
GTCCTAAAGTGTGTATTTCTCATTT44230.374565138596723No Hit
GTATCAACGCAGAGTACTTTTTTTT37250.31545447462645104No Hit
CTTTAGGACGTGAAATATGGCGAGG35860.30368315329139695No Hit
GTCCTACAGTGTGCATTTCTCATTT28610.2422859736661145No Hit
GGTATCAACGCAGAGTACTTTTTTT23150.19604754597590182No Hit
TATCAACGCAGAGTACTTTTTTTTT22830.19333760149588936No Hit
CTGAAGGACCTGGAATATGGCGAGA19520.1653066132807604No Hit
CTGTAGGACCTGGAATATGGCGAGA19040.16124169656074172No Hit
GTCCTTCAGTGTGCATTTCTCATTT17140.1451514012106677No Hit
TTTCTAAATTTTCCACCTTTTTCAG16700.14142522755065057No Hit
ATTTAGAAATGTCCACTGTAGGACG16240.13752968236063265No Hit
GAATATGGCAAGAAAACTGAAAATC16220.13736031083063185No Hit
GGAATATGGCGAGAAAACTGAAAAT13510.11441046851552628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6000.014.2872271
TCCAACG1850.013.78927818
TAGGACC7900.013.7531394
GGTTTAC550.002138889612.6863331
CGGTAGG1204.8985385E-912.459791
CCAACGA1302.1645974E-1012.3553519
CCAACGT854.1893436E-612.22709419
GGCGTGC550.00319688112.0244848
CCACCTT6500.011.91877413
GACCTAC1206.544542E-811.6291381
AGGCGTG658.400198E-411.6280727
CACCTTT7150.011.6275814
ACCTTTT7050.011.52449915
GGATAGG709.975977E-411.3918091
CTAGAGC1002.0601565E-611.337373
ACCTACA1602.3464963E-1011.2138122
TTCCACC6800.011.11506911
TAGGGTG855.6270896E-511.1150695
AAGGCGT600.00612198711.0219766
ATCCCGT1553.43789E-1010.97164810
Produced by FastQC (version 0.11.5)