FastQCFastQC Report
Thu 26 May 2016
SRR1511780_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511780_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180836
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56010.4743249697671819No Hit
CTGTAGGACGTGGAATATGGCAAGA44460.37651291119173197No Hit
GGTATCAACGCAGAGTACTTTTTTT41870.3545792980566311No Hit
TATCAACGCAGAGTACTTTTTTTTT41830.3542405549966295No Hit
GTCCTACAGTGGACATTTCTAAATT37260.31553916039145147No Hit
GTCCTAAAGTGTGTATTTCTCATTT36370.3080021273064168No Hit
CTTTAGGACGTGAAATATGGCGAGG35480.3004650942213821No Hit
CTGAAGGACCTGGAATATGGCGAGA23230.19672503209590494No Hit
GTCCTACAGTGTGCATTTCTCATTT21770.18436091040584807No Hit
CTGTAGGACCTGGAATATGGCGAGA20420.17292833213079548No Hit
TTTCTAAATTTTCCACCTTTTTCAG20050.16979495882578105No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19850.16810124352577327No Hit
ATTTAGAAATGTCCACTGTAGGACG18620.15768489443072536No Hit
GAATATGGCAAGAAAACTGAAAATC18320.15514432148071367No Hit
GGAATATGGCGAGAAAACTGAAAAT17260.14616763039067238No Hit
ACAGTGGACATTTCTAAATTTTCCA14740.12482681761057421No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14270.12084658665555591No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13010.11017618026550682No Hit
ATTCCAGGTCCTTCAGTGTGCATTT11990.10153823223546707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9350.014.5782881
TCCAACG1058.913048E-1114.47322218
TATAGCG400.0052867814.24647416
TAGGACC8300.012.70490554
CCAACGA751.4809895E-512.66406919
CGTTTCC701.0929933E-412.21126614
GTGGTAT2800.011.5745211
TAACCTG1157.0653186E-811.5652945
ACTGTTC3700.011.2945038
CCCGTTT855.3335378E-511.17370612
ACCTTTT5950.011.17370515
CCTATTC951.3592158E-511.0000733
GAACAGT4600.010.9429446
TATACTG2100.010.8572165
AAATGTC5900.010.7854677
AGGACCT16900.010.7367585
ATTTAGA6750.010.7322781
TGTCCAC6350.010.6194210
TGGTATC2600.010.5962252
GAATTAC909.191294E-510.5910641
Produced by FastQC (version 0.11.5)