##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511780_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1180836 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.811721526105234 31.0 29.0 33.0 23.0 34.0 2 29.02850014735323 31.0 29.0 33.0 24.0 34.0 3 29.048541033640575 31.0 28.0 34.0 24.0 34.0 4 32.57806333817736 35.0 32.0 37.0 26.0 37.0 5 32.05827142803912 35.0 32.0 37.0 23.0 37.0 6 32.04340399513565 35.0 32.0 37.0 23.0 37.0 7 31.783168026720052 35.0 32.0 37.0 19.0 37.0 8 31.635304987314072 35.0 32.0 37.0 18.0 37.0 9 32.787575073930675 37.0 32.0 39.0 17.0 39.0 10 32.43645857680491 37.0 32.0 39.0 17.0 39.0 11 32.483988462411375 37.0 32.0 39.0 17.0 39.0 12 32.20129975712123 37.0 32.0 39.0 15.0 39.0 13 32.19294042525804 37.0 32.0 39.0 12.0 39.0 14 32.74668031801198 38.0 32.0 40.0 10.0 41.0 15 32.68694975424191 38.0 32.0 40.0 10.0 41.0 16 32.507187280875584 37.0 31.0 40.0 10.0 41.0 17 32.43879929134952 37.0 31.0 40.0 10.0 41.0 18 32.29801936932817 37.0 31.0 40.0 10.0 41.0 19 32.330189797736516 37.0 31.0 40.0 8.0 41.0 20 32.142167074852054 37.0 31.0 40.0 2.0 41.0 21 31.933722379737745 37.0 31.0 40.0 2.0 41.0 22 32.00060550321975 37.0 31.0 40.0 2.0 41.0 23 31.80237391136449 37.0 31.0 40.0 2.0 41.0 24 31.568227933430215 37.0 31.0 40.0 2.0 41.0 25 31.00649624503318 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18660578303954267 1101 2 -0.07700947385730572 1101 3 0.23242513286071897 1101 4 0.30390787964788046 1101 5 0.2318992503195645 1101 6 0.20405300553713346 1101 7 -0.02107448784681054 1101 8 0.24760958363087227 1101 9 0.32983219956166465 1101 10 0.23152607951158188 1101 11 -0.27329891914163085 1101 12 0.26652143384673366 1101 13 0.14397052116333953 1101 14 0.25828161568993835 1101 15 -0.02793420345331299 1101 16 0.08262054420285381 1101 17 0.036616738130732074 1101 18 0.01053059810706003 1101 19 0.08569922235141547 1101 20 -0.13733625001084349 1101 21 -0.12658649242923659 1101 22 -0.19318174916101327 1101 23 -0.28552686099212465 1101 24 -0.24909660934855893 1101 25 -0.28251658141324754 1102 1 0.9740345145959708 1102 2 1.048341202071537 1102 3 0.9973662256445763 1102 4 1.1294806424067758 1102 5 0.9909524744459404 1102 6 1.089612036064402 1102 7 1.3006445643882891 1102 8 1.1907171889160715 1102 9 1.382104072388266 1102 10 1.6138184746067807 1102 11 1.5329403069843508 1102 12 1.4295140205972245 1102 13 1.6675476724036429 1102 14 2.063285828556012 1102 15 1.8293082807492311 1102 16 1.770322589012025 1102 17 1.8046637497853766 1102 18 1.8872518027394989 1102 19 2.018690243145663 1102 20 2.120664233089826 1102 21 1.935209183883174 1102 22 1.7394795966357215 1102 23 1.9621338730038964 1102 24 2.0592566576989704 1102 25 2.300222016111274 1103 1 0.48463796394843683 1103 2 0.3693951153756494 1103 3 0.5078897479304914 1103 4 0.6862294840887415 1103 5 0.6920414807277098 1103 6 0.706364249669484 1103 7 0.688454437878729 1103 8 0.3380621116740521 1103 9 0.8277505449820239 1103 10 0.482742456473332 1103 11 0.8843696277291713 1103 12 0.6576883115567185 1103 13 0.3563478922030754 1103 14 0.6469601007826071 1103 15 1.0096199963087855 1103 16 0.5420733664276298 1103 17 0.6770021160261805 1103 18 0.842380872463373 1103 19 0.9308791207948062 1103 20 0.9123318364018047 1103 21 0.6637125495619927 1103 22 0.6642213947449527 1103 23 0.8903662359726887 1103 24 1.0028987093268604 1103 25 0.9228420003756348 1104 1 0.6842174992309076 1104 2 0.6953435249221513 1104 3 0.7283298805628391 1104 4 0.833361246510421 1104 5 0.9491780340517124 1104 6 0.6799285549256098 1104 7 0.734082739503858 1104 8 1.0099716644811458 1104 9 0.8576022791625917 1104 10 0.9822299560005447 1104 11 1.0911474937604098 1104 12 1.0221196440579519 1104 13 1.0398243222243835 1104 14 1.206412046771483 1104 15 1.4064844768110731 1104 16 1.3006043244820233 1104 17 1.5936561750551448 1104 18 1.3174309878232577 1104 19 1.3520208684655444 1104 20 1.4143326685058923 1104 21 1.0604868854546723 1104 22 1.3183730898418382 1104 23 1.1053119600923615 1104 24 1.2773133997463155 1104 25 1.5200223022024808 1105 1 0.4184229328162772 1105 2 0.38945562576764914 1105 3 0.3975985964791171 1105 4 0.5507344389251898 1105 5 0.6058974835339868 1105 6 0.3788013276314288 1105 7 0.44842111331501755 1105 8 0.7038562009488025 1105 9 0.4369981114119099 1105 10 0.5601036772047152 1105 11 0.8691104853982026 1105 12 0.839429958491742 1105 13 0.8224269941937834 1105 14 0.6963286879964983 1105 15 0.8039635900998903 1105 16 0.6147384545623353 1105 17 0.7879159106416367 1105 18 0.710086739340511 1105 19 0.739464580761485 1105 20 0.8088327572121159 1105 21 0.7736002984000123 1105 22 0.7967873861507222 1105 23 1.0321841786193566 1105 24 1.097281766417094 1105 25 1.073631266358273 1106 1 -0.17262012144994543 1106 2 0.0166607100311289 1106 3 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-0.8920310896656183 2315 8 -0.585980838839486 2315 9 -0.6614651412924886 2315 10 -0.8627757508095826 2315 11 -0.9346779913184484 2315 12 -0.8479139293396365 2315 13 -0.7665279247903847 2315 14 -1.0870735126627729 2315 15 -1.2553659980889726 2315 16 -1.4727725719477185 2315 17 -1.1240716468122862 2315 18 -1.1608404160520287 2315 19 -1.4067492638924008 2315 20 -1.3260435137382522 2315 21 -1.1516469275621937 2315 22 -1.366544244844217 2315 23 -1.0792416071645654 2315 24 -1.0952805623818271 2315 25 -1.3170739660109199 2316 1 -1.9493675499796943 2316 2 -1.349609511410577 2316 3 -1.370175327340089 2316 4 -1.484731631799285 2316 5 -1.5665248979590451 2316 6 -1.5562379422534285 2316 7 -1.791971117267373 2316 8 -1.4232359816532743 2316 9 -2.24999379437023 2316 10 -1.9446763700426608 2316 11 -1.7129361905568743 2316 12 -1.6652286110162642 2316 13 -1.5248283214752831 2316 14 -1.6247939834822276 2316 15 -1.8061315493653751 2316 16 -1.948832746662486 2316 17 -1.985471099112555 2316 18 -1.7521632048408051 2316 19 -1.7182235010507192 2316 20 -2.0602841403414303 2316 21 -2.55273437887978 2316 22 -2.261859894749925 2316 23 -2.3213133283723977 2316 24 -1.8248353937726804 2316 25 -1.9821880942073413 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50749.0 3 4.0 4 806.0 5 6131.0 6 7570.0 7 6108.0 8 4964.0 9 4590.0 10 4179.0 11 4046.0 12 3634.0 13 3632.0 14 3542.0 15 3519.0 16 3589.0 17 3813.0 18 4000.0 19 4500.0 20 5554.0 21 6893.0 22 8937.0 23 10747.0 24 12814.0 25 15155.0 26 17363.0 27 19837.0 28 22952.0 29 27342.0 30 34056.0 31 42920.0 32 53894.0 33 68178.0 34 86426.0 35 112565.0 36 149394.0 37 196701.0 38 169732.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.93124431639512 23.476963871395427 15.336596891121253 21.25519492108819 2 16.188784894769466 24.274412365476664 40.77611116192257 18.7606915778313 3 20.757666602305484 27.88270344061326 31.007438797597636 20.35219115948362 4 15.006995044189031 20.648760708514985 38.18650515397566 26.157739093320327 5 12.246147799474656 40.40551975801294 36.235969570347606 11.112362872164796 6 29.836912153762253 40.51612586337137 17.912309584057397 11.73465239880898 7 26.55432253081715 33.195464907912694 23.572621430918435 16.67759113035172 8 23.77612132421437 40.953273782303384 20.616495432049835 14.65410946143241 9 26.51214901984695 15.296535674725364 21.498243617233893 36.69307168819379 10 15.007333787249033 31.155723572113313 35.272044551487255 18.564898089150397 11 33.60602149663458 22.25423344139237 26.97453329674908 17.165211765223958 12 23.836502274659647 25.89402762110911 33.19072250507268 17.07874759915856 13 29.94276936001274 23.51257922353316 26.316694274226055 20.22795714222805 14 19.794789454251056 25.168185929290775 28.526992740736222 26.510031875721946 15 21.86247709250057 35.699877036269214 23.331182314902325 19.106463556327892 16 20.078825510062362 29.93709541375771 30.69833575534621 19.285743320833713 17 20.31721593853846 31.39775548848443 29.152820544089103 19.13220802888801 18 19.989482027986952 28.339498457025364 33.57231656216443 18.098702952823253 19 23.507498077633134 27.31911967453567 29.181867761484238 19.99151448634696 20 23.356079929812438 29.00843131476344 29.956403768177797 17.679084987246323 21 21.798115911100272 27.77396691835276 28.67934243197193 21.748574738575044 22 22.679017238634323 30.477221222930197 29.04510025100861 17.798661287426874 23 20.608365598609797 30.353410634499628 30.076826926008355 18.961396840882223 24 22.962865098884127 28.063747908155314 30.816259815578906 18.157127177381653 25 21.612587702769652 29.591179123247535 30.194735081531288 18.601498092451525 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 222.0 1 222.0 2 388.5 3 555.0 4 555.0 5 555.0 6 1380.5 7 2206.0 8 2206.0 9 2206.0 10 3111.0 11 4016.0 12 4016.0 13 4016.0 14 5916.0 15 7816.0 16 7816.0 17 7816.0 18 13846.0 19 19876.0 20 19876.0 21 19876.0 22 32034.0 23 44192.0 24 44192.0 25 44192.0 26 64332.5 27 84473.0 28 84473.0 29 84473.0 30 107022.0 31 129571.0 32 129571.0 33 129571.0 34 138718.0 35 147865.0 36 147865.0 37 147865.0 38 149063.0 39 150261.0 40 150261.0 41 150261.0 42 150271.5 43 150282.0 44 150282.0 45 150282.0 46 145990.5 47 141699.0 48 141699.0 49 141699.0 50 129220.5 51 116742.0 52 116742.0 53 116742.0 54 96842.0 55 76942.0 56 76942.0 57 76942.0 58 63060.0 59 49178.0 60 49178.0 61 49178.0 62 39013.5 63 28849.0 64 28849.0 65 28849.0 66 22097.0 67 15345.0 68 15345.0 69 15345.0 70 10984.5 71 6624.0 72 6624.0 73 6624.0 74 4692.0 75 2760.0 76 2760.0 77 2760.0 78 1839.5 79 919.0 80 919.0 81 919.0 82 621.5 83 324.0 84 324.0 85 324.0 86 210.5 87 97.0 88 97.0 89 97.0 90 57.5 91 18.0 92 18.0 93 18.0 94 10.5 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3561883275916385 2 0.0 3 0.0 4 0.0 5 0.023457956905107907 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005081145900023373 25 8.468576500038955E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1180836.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.11697420930132 #Duplication Level Percentage of deduplicated Percentage of total 1 91.33426701132596 67.69419512503887 2 5.972633727857567 8.85347079938445 3 1.2327152129990375 2.740953649477891 4 0.4408516520000226 1.3069836208565422 5 0.24966485340068553 0.9252201750233804 6 0.14998763787899475 0.6669977933034887 7 0.10832647022096381 0.5620181139686276 8 0.07845977615342352 0.46521609645046774 9 0.05679434467842687 0.3788482481788621 >10 0.3182248297317787 4.316383397144961 >50 0.026271246769799167 1.3747532794478152 >100 0.02544260113896271 3.9768625820355714 >500 0.003407483488090702 1.8190505067746399 >1k 0.0028395695734089186 4.44753134595571 >5k 1.1358278293635674E-4 0.471515266958754 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5601 0.4743249697671819 No Hit CTGTAGGACGTGGAATATGGCAAGA 4446 0.37651291119173197 No Hit GGTATCAACGCAGAGTACTTTTTTT 4187 0.3545792980566311 No Hit TATCAACGCAGAGTACTTTTTTTTT 4183 0.3542405549966295 No Hit GTCCTACAGTGGACATTTCTAAATT 3726 0.31553916039145147 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3637 0.3080021273064168 No Hit CTTTAGGACGTGAAATATGGCGAGG 3548 0.3004650942213821 No Hit CTGAAGGACCTGGAATATGGCGAGA 2323 0.19672503209590494 No Hit GTCCTACAGTGTGCATTTCTCATTT 2177 0.18436091040584807 No Hit CTGTAGGACCTGGAATATGGCGAGA 2042 0.17292833213079548 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2005 0.16979495882578105 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1985 0.16810124352577327 No Hit ATTTAGAAATGTCCACTGTAGGACG 1862 0.15768489443072536 No Hit GAATATGGCAAGAAAACTGAAAATC 1832 0.15514432148071367 No Hit GGAATATGGCGAGAAAACTGAAAAT 1726 0.14616763039067238 No Hit ACAGTGGACATTTCTAAATTTTCCA 1474 0.12482681761057421 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1427 0.12084658665555591 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1301 0.11017618026550682 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1199 0.10153823223546707 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.693715300007791E-4 0.0 0.0 0.0 0.0 7 1.693715300007791E-4 0.0 0.0 8.468576500038955E-5 0.0 8 1.693715300007791E-4 0.0 0.0 8.468576500038955E-5 0.0 9 1.693715300007791E-4 0.0 0.0 1.693715300007791E-4 0.0 10 1.693715300007791E-4 8.468576500038955E-5 0.0 1.693715300007791E-4 0.0 11 1.693715300007791E-4 8.468576500038955E-5 0.0 1.693715300007791E-4 0.0 12 1.693715300007791E-4 8.468576500038955E-5 0.0 1.693715300007791E-4 0.0 13 1.693715300007791E-4 8.468576500038955E-5 0.0 1.693715300007791E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 935 0.0 14.578288 1 TCCAACG 105 8.913048E-11 14.473222 18 TATAGCG 40 0.00528678 14.246474 16 TAGGACC 830 0.0 12.7049055 4 CCAACGA 75 1.4809895E-5 12.664069 19 CGTTTCC 70 1.0929933E-4 12.211266 14 GTGGTAT 280 0.0 11.574521 1 TAACCTG 115 7.0653186E-8 11.565294 5 ACTGTTC 370 0.0 11.294503 8 CCCGTTT 85 5.3335378E-5 11.173706 12 ACCTTTT 595 0.0 11.173705 15 CCTATTC 95 1.3592158E-5 11.000073 3 GAACAGT 460 0.0 10.942944 6 TATACTG 210 0.0 10.857216 5 AAATGTC 590 0.0 10.785467 7 AGGACCT 1690 0.0 10.736758 5 ATTTAGA 675 0.0 10.732278 1 TGTCCAC 635 0.0 10.61942 10 TGGTATC 260 0.0 10.596225 2 GAATTAC 90 9.191294E-5 10.591064 1 >>END_MODULE