FastQCFastQC Report
Thu 26 May 2016
SRR1511779_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511779_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173396
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39610.3375671981155552No Hit
GTCCTACAGTGGACATTTCTAAATT30250.25779873120412883No Hit
GTCCTAAAGTGTGTATTTCTCATTT28610.2438222049504174No Hit
GGTATCAACGCAGAGTACTTTTTTT26950.22967523325458755No Hit
CTGTAGGACGTGGAATATGGCAAGA26480.22566976536480438No Hit
TATCAACGCAGAGTACTTTTTTTTT25530.21757360686417887No Hit
CTTTAGGACGTGAAATATGGCGAGG24570.2093922256424941No Hit
GTCCTACAGTGTGCATTTCTCATTT15810.13473712199462076No Hit
CTGAAGGACCTGGAATATGGCGAGA12730.10848852390838216No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12380.10550572867130961No Hit
ATTTAGAAATGTCCACTGTAGGACG12320.1049943923449543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6750.015.3532221
CGTTAGG400.003842824114.9472471
TAGGACC3750.012.5974064
GCCTTAA655.368628E-412.2644071
GCCGGTT701.1477122E-412.14749911
GTCTCGA550.00319623612.02479711
GTCTTAA1001.0840286E-611.9577971
AAATGTC3950.011.7203727
AATGTCC4100.011.5220188
ATTTAGA4800.011.2104361
GTATTGG1352.8130671E-811.0720361
AATCCCG951.4437555E-510.94031919
AGGACCT8800.010.8437915
TCCCGCC700.0015606910.79777713
TCCAACG803.963458E-410.62951518
GGCCTAA750.001779292410.6291541
ATTAGGG803.9650183E-410.6290623
GGCCTAC854.243901E-410.5509981
CACCTTT5150.010.45707114
GTATCAA13700.010.4012471
Produced by FastQC (version 0.11.5)