##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511779_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1173396 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.69269027676931 31.0 31.0 34.0 27.0 34.0 2 27.95690968777804 31.0 28.0 34.0 10.0 34.0 3 29.71033649339183 31.0 30.0 34.0 16.0 34.0 4 34.15891480795912 37.0 35.0 37.0 31.0 37.0 5 34.52462169634122 37.0 35.0 37.0 32.0 37.0 6 34.74482016301402 37.0 35.0 37.0 32.0 37.0 7 34.76551309191441 37.0 35.0 37.0 32.0 37.0 8 34.792838052967625 37.0 35.0 37.0 32.0 37.0 9 36.483438668616564 39.0 37.0 39.0 33.0 39.0 10 36.38934000115903 39.0 37.0 39.0 32.0 39.0 11 36.39878267865239 39.0 37.0 39.0 32.0 39.0 12 36.3107706179329 39.0 37.0 39.0 32.0 39.0 13 36.310633409352 39.0 37.0 39.0 32.0 39.0 14 37.575734023296484 40.0 38.0 41.0 32.0 41.0 15 37.5305745034072 40.0 38.0 41.0 32.0 41.0 16 37.497031692625505 40.0 38.0 41.0 32.0 41.0 17 37.472999737514016 40.0 38.0 41.0 32.0 41.0 18 37.450947506212735 40.0 38.0 41.0 32.0 41.0 19 37.481902955183074 40.0 38.0 41.0 32.0 41.0 20 37.439248131065725 40.0 38.0 41.0 32.0 41.0 21 37.35279564614163 40.0 38.0 41.0 32.0 41.0 22 37.28752271185516 40.0 38.0 41.0 32.0 41.0 23 37.20353486802409 40.0 38.0 41.0 32.0 41.0 24 37.12907748108908 40.0 38.0 41.0 32.0 41.0 25 37.052295218323565 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6130684859692117 1101 2 3.298404577811276 1101 3 1.7412847881205096 1101 4 0.8133952697823759 1101 5 0.1949969223728374 1101 6 0.2782032027592507 1101 7 0.10162703300543541 1101 8 0.1749673939619143 1101 9 0.08285174573057219 1101 10 0.05739102870033008 1101 11 0.11160065935805363 1101 12 0.08464667506554235 1101 13 0.10826788802395271 1101 14 0.03431667792192883 1101 15 0.270755473958026 1101 16 0.10091388889699004 1101 17 0.10682310893172087 1101 18 0.22393101915459823 1101 19 0.2835223358130321 1101 20 0.3397578341487133 1101 21 0.3311865939045262 1101 22 0.3198367499886956 1101 23 0.2832679987246465 1101 24 0.31473586647428675 1101 25 0.34374286920969155 1102 1 1.1077817863578119 1102 2 3.985316084504955 1102 3 2.2587598659159944 1102 4 1.3115817175324622 1102 5 0.9498054122606803 1102 6 0.8175546473170883 1102 7 0.6571188412288862 1102 8 0.5904688040836277 1102 9 0.6627454461815105 1102 10 0.7587971286706647 1102 11 0.6941260842797519 1102 12 0.8476708205720058 1102 13 0.8963122678971658 1102 14 0.9600006246540147 1102 15 0.8351931345133323 1102 16 0.8855636709690913 1102 17 0.6893265399765198 1102 18 0.7920881584927244 1102 19 0.873223420174007 1102 20 0.9444549721797699 1102 21 1.0123319344702821 1102 22 0.9103987375313665 1102 23 0.9093551246402214 1102 24 0.6872235373352922 1102 25 0.7994076121452593 1103 1 1.0752281844918379 1103 2 3.8392802801530124 1103 3 2.1685549777049147 1103 4 1.2478849580655407 1103 5 0.8843244158789929 1103 6 0.7943022525521783 1103 7 0.7966992465197933 1103 8 0.6602023148438718 1103 9 0.721860753738099 1103 10 0.8211108553335436 1103 11 0.8051437472284277 1103 12 0.7596401596069171 1103 13 0.8071851484928416 1103 14 0.8585271340287406 1103 15 0.8584442833027381 1103 16 0.8743723190358352 1103 17 0.8269918809252559 1103 18 0.9581991200867748 1103 19 0.9496690009481057 1103 20 0.8800554825313327 1103 21 0.9481517365096934 1103 22 0.9640105713082647 1103 23 0.9058302599531629 1103 24 0.8977996265670711 1103 25 1.0633712595641 1104 1 0.9717211639401775 1104 2 3.6884041278445387 1104 3 1.9786142720086595 1104 4 1.1537519353099341 1104 5 0.8724571155180172 1104 6 0.7156462728458237 1104 7 0.6167811830973164 1104 8 0.560458155658317 1104 9 0.48894915988117305 1104 10 0.7191820422564845 1104 11 0.6540288744616092 1104 12 0.577495244232118 1104 13 0.5246920388333436 1104 14 0.6793348772523302 1104 15 0.6635314403832098 1104 16 0.6946158139033471 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16 0.6786747394265404 1107 17 0.6275314829749519 1107 18 0.6403849303399554 1107 19 0.7900458288376413 1107 20 0.7129823768782941 1107 21 0.792012149848837 1107 22 0.7438490878245858 1107 23 0.7629877435808012 1107 24 0.9266648613274739 1107 25 1.0260216885278695 1108 1 0.9989906288649166 1108 2 3.8267339562450324 1108 3 2.156224024923443 1108 4 1.3199221395329204 1108 5 0.8199998146341727 1108 6 0.49930115658076346 1108 7 0.4618736079252912 1108 8 0.41970540650276433 1108 9 0.4684390290359275 1108 10 0.39013284102984613 1108 11 0.392576778277558 1108 12 0.5027673993391986 1108 13 0.4974865661191217 1108 14 0.5348128614224166 1108 15 0.5693499634931811 1108 16 0.5806507059698163 1108 17 0.5876750534311554 1108 18 0.6644558424695646 1108 19 0.45593260501186705 1108 20 0.33986774007951936 1108 21 0.4245552171109992 1108 22 0.3083686124250349 1108 23 0.4770977126607221 1108 24 0.4386576957203232 1108 25 0.5964288159046731 1109 1 0.537451401544768 1109 2 2.9153886554796813 1109 3 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-0.768663091085223 2315 10 -0.8133673589815871 2315 11 -0.7223869339345725 2315 12 -0.8019157254107867 2315 13 -0.7397755631454075 2315 14 -0.9464889272512238 2315 15 -0.8484167660342194 2315 16 -0.9234038114311787 2315 17 -0.9594219235389545 2315 18 -0.9674659816346676 2315 19 -0.7717089745069643 2315 20 -0.8557405803959384 2315 21 -0.9321423084618843 2315 22 -1.077633444835378 2315 23 -0.96590188731642 2315 24 -1.0546219202577305 2315 25 -1.0151889908271414 2316 1 -1.3057229703347453 2316 2 -1.135397361156965 2316 3 -0.6446114472106146 2316 4 -0.7308180542394496 2316 5 -1.0175818418992435 2316 6 -0.9816774117427514 2316 7 -0.9006412048825041 2316 8 -1.0398846583460752 2316 9 -1.0663137533899842 2316 10 -1.3163725868593232 2316 11 -1.3071475099633076 2316 12 -1.0483107348722882 2316 13 -1.124606699487174 2316 14 -1.3361325976704137 2316 15 -1.8668105567266693 2316 16 -1.5146767836239974 2316 17 -1.3655879327117972 2316 18 -1.2873928554920724 2316 19 -1.5306733415488836 2316 20 -1.5709591570019867 2316 21 -1.5762665587585616 2316 22 -1.5676459532545053 2316 23 -1.709068762456738 2316 24 -1.4291480436099917 2316 25 -1.4836228726065457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24821.0 3 0.0 4 208.0 5 1163.0 6 1387.0 7 1295.0 8 1160.0 9 1079.0 10 997.0 11 992.0 12 950.0 13 994.0 14 991.0 15 1027.0 16 1001.0 17 1102.0 18 1229.0 19 1491.0 20 1916.0 21 2481.0 22 2995.0 23 3479.0 24 4079.0 25 4714.0 26 5354.0 27 6477.0 28 7564.0 29 9413.0 30 12061.0 31 16339.0 32 22645.0 33 32800.0 34 49537.0 35 79427.0 36 137808.0 37 199352.0 38 533068.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.01480148219356 19.379646768865754 12.938428288489137 25.667123460451545 2 15.432889965898038 21.21062859423341 40.929273935639515 22.427207504229038 3 17.680390933666043 25.287541460853795 30.55226027700793 26.479807328472226 4 11.594125086501062 17.09806408066842 39.31076976570569 31.997041067124826 5 12.277119379300588 37.91224712072584 36.66517241555635 13.145461084417235 6 29.979333296403144 37.94713585055203 18.925842754083273 13.147688098961552 7 26.300754391526816 31.877984925805098 23.663196397465136 18.15806428520295 8 23.672997010386947 37.72187735427767 21.52564010785788 17.07948552747751 9 25.815837108699874 14.96596204520895 21.16523322049845 38.05296762559273 10 14.687454192787431 28.695086739685493 35.51281920170173 21.104639865825348 11 32.854892977306896 22.133192886289027 26.382653426464724 18.629260709939356 12 22.80406614646718 24.424064851081816 33.22859460915156 19.54327439329945 13 28.39325205919575 22.39459005705666 26.540593747203626 22.67156413654396 14 19.952599122546864 22.688078023105586 28.646935902287034 28.712386952060516 15 21.813948573201202 32.18793996229747 24.07337335392314 21.924738110578186 16 20.769032790294155 27.944871126201214 29.599811146450133 21.6862849370545 17 20.469273590662254 28.948906925927314 29.183271006691736 21.398548476718698 18 20.39345625858619 27.12204575437448 31.863156172340794 20.621341814698532 19 22.62053496097645 26.488545194538236 28.818623582530677 22.072296261954637 20 22.659872659233486 27.41439951184051 29.54038181776345 20.385346011162557 21 21.761362619493823 26.724345846681448 28.257429134633334 23.256862399191398 22 22.12504548944943 28.679446609661746 28.51521584797021 20.68029205291861 23 20.88587314086634 28.191676126388703 29.425104568278737 21.497346164466215 24 22.24939938007672 26.644769642338183 30.035853962642676 21.069977014942417 25 21.541808847542327 27.473776789945013 29.75929530555963 21.22511905695303 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 333.0 1 333.0 2 408.0 3 483.0 4 483.0 5 483.0 6 1166.5 7 1850.0 8 1850.0 9 1850.0 10 2472.0 11 3094.0 12 3094.0 13 3094.0 14 4805.5 15 6517.0 16 6517.0 17 6517.0 18 11512.0 19 16507.0 20 16507.0 21 16507.0 22 26050.0 23 35593.0 24 35593.0 25 35593.0 26 51001.5 27 66410.0 28 66410.0 29 66410.0 30 85466.0 31 104522.0 32 104522.0 33 104522.0 34 114943.0 35 125364.0 36 125364.0 37 125364.0 38 130916.0 39 136468.0 40 136468.0 41 136468.0 42 140556.5 43 144645.0 44 144645.0 45 144645.0 46 146353.0 47 148061.0 48 148061.0 49 148061.0 50 139917.5 51 131774.0 52 131774.0 53 131774.0 54 115217.5 55 98661.0 56 98661.0 57 98661.0 58 83634.5 59 68608.0 60 68608.0 61 68608.0 62 56022.0 63 43436.0 64 43436.0 65 43436.0 66 33527.5 67 23619.0 68 23619.0 69 23619.0 70 17224.5 71 10830.0 72 10830.0 73 10830.0 74 7620.0 75 4410.0 76 4410.0 77 4410.0 78 2987.0 79 1564.0 80 1564.0 81 1564.0 82 994.5 83 425.0 84 425.0 85 425.0 86 287.5 87 150.0 88 150.0 89 150.0 90 99.5 91 49.0 92 49.0 93 49.0 94 31.0 95 13.0 96 13.0 97 13.0 98 11.5 99 10.0 100 10.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.185802576453303 3 0.0 4 0.0 5 8.522272105921615E-4 6 9.374499316513777E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 8.522272105921615E-5 14 0.0 15 0.0 16 0.0 17 6.817817684737292E-4 18 0.0 19 1.704454421184323E-4 20 7.670044895329455E-4 21 7.670044895329455E-4 22 0.004005467889783159 23 0.0 24 0.0021305680264804036 25 6.817817684737292E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1173396.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.61101015321871 #Duplication Level Percentage of deduplicated Percentage of total 1 86.90314768385893 64.83931634187064 2 9.316020345806884 13.901553772171793 3 2.010970827423701 4.501216944682092 4 0.713246991599162 2.128643141278311 5 0.31820336380447856 1.187073720380215 6 0.16425601226281236 0.7353184199200746 7 0.10874113476248225 0.5679300136885261 8 0.08337888228452414 0.4976786106155727 9 0.06113216612946047 0.4105019400996086 >10 0.27579301429279257 3.6470840011453856 >50 0.021079481081518272 1.079551040996975 >100 0.020120905441617237 2.9110309232581724 >500 0.0021845480523978874 1.023242456186192 >1k 0.0017246431992614902 2.5698586737063844 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3961 0.3375671981155552 No Hit GTCCTACAGTGGACATTTCTAAATT 3025 0.25779873120412883 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2861 0.2438222049504174 No Hit GGTATCAACGCAGAGTACTTTTTTT 2695 0.22967523325458755 No Hit CTGTAGGACGTGGAATATGGCAAGA 2648 0.22566976536480438 No Hit TATCAACGCAGAGTACTTTTTTTTT 2553 0.21757360686417887 No Hit CTTTAGGACGTGAAATATGGCGAGG 2457 0.2093922256424941 No Hit GTCCTACAGTGTGCATTTCTCATTT 1581 0.13473712199462076 No Hit CTGAAGGACCTGGAATATGGCGAGA 1273 0.10848852390838216 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1238 0.10550572867130961 No Hit ATTTAGAAATGTCCACTGTAGGACG 1232 0.1049943923449543 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 8.522272105921615E-5 13 0.0 0.0 0.0 0.0 8.522272105921615E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 675 0.0 15.353222 1 CGTTAGG 40 0.0038428241 14.947247 1 TAGGACC 375 0.0 12.597406 4 GCCTTAA 65 5.368628E-4 12.264407 1 GCCGGTT 70 1.1477122E-4 12.147499 11 GTCTCGA 55 0.003196236 12.024797 11 GTCTTAA 100 1.0840286E-6 11.957797 1 AAATGTC 395 0.0 11.720372 7 AATGTCC 410 0.0 11.522018 8 ATTTAGA 480 0.0 11.210436 1 GTATTGG 135 2.8130671E-8 11.072036 1 AATCCCG 95 1.4437555E-5 10.940319 19 AGGACCT 880 0.0 10.843791 5 TCCCGCC 70 0.00156069 10.797777 13 TCCAACG 80 3.963458E-4 10.629515 18 GGCCTAA 75 0.0017792924 10.629154 1 ATTAGGG 80 3.9650183E-4 10.629062 3 GGCCTAC 85 4.243901E-4 10.550998 1 CACCTTT 515 0.0 10.457071 14 GTATCAA 1370 0.0 10.401247 1 >>END_MODULE