FastQCFastQC Report
Thu 26 May 2016
SRR1511779_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511779_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173396
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51720.44077191331826593No Hit
TATCAACGCAGAGTACTTTTTTTTT40920.34873137457431247No Hit
GGTATCAACGCAGAGTACTTTTTTT40530.34540768845300307No Hit
CTGTAGGACGTGGAATATGGCAAGA26320.22430620182785693No Hit
CTTTAGGACGTGAAATATGGCGAGG23860.20334141244728976No Hit
GTCCTACAGTGGACATTTCTAAATT23250.19814282646267756No Hit
GTCCTAAAGTGTGTATTTCTCATTT22530.192006790546414No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18950.1614970564072146No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15300.13039076322060072No Hit
CTGAAGGACCTGGAATATGGCGAGA14440.12306160920950812No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13450.11462455982464573No Hit
TTTCTAAATTTTCCACCTTTTTCAG13110.11172698730863238No Hit
ATTTAGAAATGTCCACTGTAGGACG12260.10448305601859902No Hit
GTCCTACAGTGTGCATTTCTCATTT12180.10380127425012528No Hit
CTGTAGGACCTGGAATATGGCGAGA11750.10013669724457898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGT508.728558E-515.196426
GGTATCA10400.014.7567181
CCAACGA500.001500864413.29800119
TAGGACC4800.013.2595544
CACCTTT4500.012.03049914
ACTGATC1201.2779674E-711.0807238
GCGCCAC600.005884512311.08072313
CCGTCTC600.005884512311.0807239
TAGAAAT4900.011.0503024
TCCAACG951.3615629E-510.99834618
CCACCTT4900.010.85458713
GGCGAGG8450.010.79127719
AAGTCGT1158.834395E-710.7366017
ATTTAGA5150.010.73539451
AATGTCC5100.010.6151478
CTAGAGT909.51587E-510.5548694
TTCCACC4900.010.46692311
TTTAGAA5650.010.4241012
TAAGATT1106.044662E-610.3629624
AAATGTC4800.010.2892437
Produced by FastQC (version 0.11.5)